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Trajectory of chemical cocktail-induced neutrophil reprogramming
Hematopoietic reprogramming holds great promise for generating functional target cells and provides new angle for understanding hematopoiesis. We reported before for the first time that diverse differentiated hematopoietic cell lineages could be reprogrammed back into hematopoietic stem/progenitor c...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727137/ https://www.ncbi.nlm.nih.gov/pubmed/33302977 http://dx.doi.org/10.1186/s13045-020-01008-8 |
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author | Zhou, Yi Wei, Chuijin Xiong, Shumin Dong, Liaoliao Chen, Zhu Chen, Sai-Juan Cheng, Lin |
author_facet | Zhou, Yi Wei, Chuijin Xiong, Shumin Dong, Liaoliao Chen, Zhu Chen, Sai-Juan Cheng, Lin |
author_sort | Zhou, Yi |
collection | PubMed |
description | Hematopoietic reprogramming holds great promise for generating functional target cells and provides new angle for understanding hematopoiesis. We reported before for the first time that diverse differentiated hematopoietic cell lineages could be reprogrammed back into hematopoietic stem/progenitor cell-like cells by chemical cocktail. However, the exact cell types of induced cells and reprogramming trajectory remain elusive. Here, based on genetic tracing method CellTagging and single-cell RNA sequencing, it is found that neutrophils could be reprogrammed into multipotent progenitors, which acquire multi-differentiation potential both in vitro and in vivo, including into lymphoid cells. Construction of trajectory map of the reprogramming procession shows that mature neutrophils follow their canonical developmental route reversely into immature ones, premature ones, granulocyte/monocyte progenitors, common myeloid progenitors, and then the terminal cells, which is stage by stage or skips intermediate stages. Collectively, this study provides a precise dissection of hematopoietic reprogramming procession and sheds light on chemical cocktail-induction of hematopoietic stem cells. |
format | Online Article Text |
id | pubmed-7727137 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77271372020-12-10 Trajectory of chemical cocktail-induced neutrophil reprogramming Zhou, Yi Wei, Chuijin Xiong, Shumin Dong, Liaoliao Chen, Zhu Chen, Sai-Juan Cheng, Lin J Hematol Oncol Letter to the Editor Hematopoietic reprogramming holds great promise for generating functional target cells and provides new angle for understanding hematopoiesis. We reported before for the first time that diverse differentiated hematopoietic cell lineages could be reprogrammed back into hematopoietic stem/progenitor cell-like cells by chemical cocktail. However, the exact cell types of induced cells and reprogramming trajectory remain elusive. Here, based on genetic tracing method CellTagging and single-cell RNA sequencing, it is found that neutrophils could be reprogrammed into multipotent progenitors, which acquire multi-differentiation potential both in vitro and in vivo, including into lymphoid cells. Construction of trajectory map of the reprogramming procession shows that mature neutrophils follow their canonical developmental route reversely into immature ones, premature ones, granulocyte/monocyte progenitors, common myeloid progenitors, and then the terminal cells, which is stage by stage or skips intermediate stages. Collectively, this study provides a precise dissection of hematopoietic reprogramming procession and sheds light on chemical cocktail-induction of hematopoietic stem cells. BioMed Central 2020-12-10 /pmc/articles/PMC7727137/ /pubmed/33302977 http://dx.doi.org/10.1186/s13045-020-01008-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Letter to the Editor Zhou, Yi Wei, Chuijin Xiong, Shumin Dong, Liaoliao Chen, Zhu Chen, Sai-Juan Cheng, Lin Trajectory of chemical cocktail-induced neutrophil reprogramming |
title | Trajectory of chemical cocktail-induced neutrophil reprogramming |
title_full | Trajectory of chemical cocktail-induced neutrophil reprogramming |
title_fullStr | Trajectory of chemical cocktail-induced neutrophil reprogramming |
title_full_unstemmed | Trajectory of chemical cocktail-induced neutrophil reprogramming |
title_short | Trajectory of chemical cocktail-induced neutrophil reprogramming |
title_sort | trajectory of chemical cocktail-induced neutrophil reprogramming |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727137/ https://www.ncbi.nlm.nih.gov/pubmed/33302977 http://dx.doi.org/10.1186/s13045-020-01008-8 |
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