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Behaviour of Human Oral Epithelial Cells Grown on Invisalign(®) SmartTrack(®) Material

Invisalign aligners have been widely used to correct malocclusions, but their effect on oral cells is poorly known. Previous research evaluated the impact of aligners’ eluates on various cells, but the cell behavior in direct contact with aligners is not yet studied. In the present study, we seeded...

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Autores principales: Nemec, Michael, Bartholomaeus, Hans Magnus, H. Bertl, Michael, Behm, Christian, Ali Shokoohi-Tabrizi, Hassan, Jonke, Erwin, Andrukhov, Oleh, Rausch-Fan, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727678/
https://www.ncbi.nlm.nih.gov/pubmed/33255259
http://dx.doi.org/10.3390/ma13235311
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author Nemec, Michael
Bartholomaeus, Hans Magnus
H. Bertl, Michael
Behm, Christian
Ali Shokoohi-Tabrizi, Hassan
Jonke, Erwin
Andrukhov, Oleh
Rausch-Fan, Xiaohui
author_facet Nemec, Michael
Bartholomaeus, Hans Magnus
H. Bertl, Michael
Behm, Christian
Ali Shokoohi-Tabrizi, Hassan
Jonke, Erwin
Andrukhov, Oleh
Rausch-Fan, Xiaohui
author_sort Nemec, Michael
collection PubMed
description Invisalign aligners have been widely used to correct malocclusions, but their effect on oral cells is poorly known. Previous research evaluated the impact of aligners’ eluates on various cells, but the cell behavior in direct contact with aligners is not yet studied. In the present study, we seeded oral epithelial cells (cell line Ca9-22) directly on Invisalign SmartTrack material. This material is composed of polyurethane and co-polyester and exhibit better mechanical characteristics compared to the predecessor. Cell morphology and behavior were investigated by scanning electron microscopy and an optical cell moves analyzer. The effect of aligners on cell proliferation/viability was assessed by cell-counting kit (CCK)-8 and 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay and live/dead staining. The expression of inflammatory markers and proteins involved in epithelial barrier function was measured by qPCR. Cells formed cluster-like structures on aligners. The proliferation/viability of cells growing on aligners was significantly lower (p < 0.05) compared to those growing on tissue culture plastic (TCP). Live/dead staining revealed a rare occurrence of dead cells on aligners. The gene expression level of all inflammatory markers in cells grown on aligners’ surfaces was significantly increased (p < 0.05) compared to cells grown on TCP after two days. Gene expression levels of the proteins involved in barrier function significantly increased (p < 0.05) on aligners’ surfaces after two and seven days of culture. Aligners’ material exhibits no cytotoxic effect on oral epithelial cells, but alters their behavior and the expression of proteins involved in the inflammatory response, and barrier function. The clinical relevance of these effects has still to be established.
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spelling pubmed-77276782020-12-11 Behaviour of Human Oral Epithelial Cells Grown on Invisalign(®) SmartTrack(®) Material Nemec, Michael Bartholomaeus, Hans Magnus H. Bertl, Michael Behm, Christian Ali Shokoohi-Tabrizi, Hassan Jonke, Erwin Andrukhov, Oleh Rausch-Fan, Xiaohui Materials (Basel) Article Invisalign aligners have been widely used to correct malocclusions, but their effect on oral cells is poorly known. Previous research evaluated the impact of aligners’ eluates on various cells, but the cell behavior in direct contact with aligners is not yet studied. In the present study, we seeded oral epithelial cells (cell line Ca9-22) directly on Invisalign SmartTrack material. This material is composed of polyurethane and co-polyester and exhibit better mechanical characteristics compared to the predecessor. Cell morphology and behavior were investigated by scanning electron microscopy and an optical cell moves analyzer. The effect of aligners on cell proliferation/viability was assessed by cell-counting kit (CCK)-8 and 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay and live/dead staining. The expression of inflammatory markers and proteins involved in epithelial barrier function was measured by qPCR. Cells formed cluster-like structures on aligners. The proliferation/viability of cells growing on aligners was significantly lower (p < 0.05) compared to those growing on tissue culture plastic (TCP). Live/dead staining revealed a rare occurrence of dead cells on aligners. The gene expression level of all inflammatory markers in cells grown on aligners’ surfaces was significantly increased (p < 0.05) compared to cells grown on TCP after two days. Gene expression levels of the proteins involved in barrier function significantly increased (p < 0.05) on aligners’ surfaces after two and seven days of culture. Aligners’ material exhibits no cytotoxic effect on oral epithelial cells, but alters their behavior and the expression of proteins involved in the inflammatory response, and barrier function. The clinical relevance of these effects has still to be established. MDPI 2020-11-24 /pmc/articles/PMC7727678/ /pubmed/33255259 http://dx.doi.org/10.3390/ma13235311 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nemec, Michael
Bartholomaeus, Hans Magnus
H. Bertl, Michael
Behm, Christian
Ali Shokoohi-Tabrizi, Hassan
Jonke, Erwin
Andrukhov, Oleh
Rausch-Fan, Xiaohui
Behaviour of Human Oral Epithelial Cells Grown on Invisalign(®) SmartTrack(®) Material
title Behaviour of Human Oral Epithelial Cells Grown on Invisalign(®) SmartTrack(®) Material
title_full Behaviour of Human Oral Epithelial Cells Grown on Invisalign(®) SmartTrack(®) Material
title_fullStr Behaviour of Human Oral Epithelial Cells Grown on Invisalign(®) SmartTrack(®) Material
title_full_unstemmed Behaviour of Human Oral Epithelial Cells Grown on Invisalign(®) SmartTrack(®) Material
title_short Behaviour of Human Oral Epithelial Cells Grown on Invisalign(®) SmartTrack(®) Material
title_sort behaviour of human oral epithelial cells grown on invisalign(®) smarttrack(®) material
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727678/
https://www.ncbi.nlm.nih.gov/pubmed/33255259
http://dx.doi.org/10.3390/ma13235311
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