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Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes

As a common injury almost all cells face, DNA damage in oocytes—especially double-strand breaks (DSBs), which occur naturally during the first meiosis phase (meiosis I) due to synaptic complex separation—affects the fertilization ability of oocytes, instead of causing cancer (as in somatic cells). T...

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Detalles Bibliográficos
Autores principales: Wang, Lili, Xu, Xiaolei, Teng, Mingming, Zhao, Guimin, Lei, Anmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727702/
https://www.ncbi.nlm.nih.gov/pubmed/33255251
http://dx.doi.org/10.3390/ijms21238892
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author Wang, Lili
Xu, Xiaolei
Teng, Mingming
Zhao, Guimin
Lei, Anmin
author_facet Wang, Lili
Xu, Xiaolei
Teng, Mingming
Zhao, Guimin
Lei, Anmin
author_sort Wang, Lili
collection PubMed
description As a common injury almost all cells face, DNA damage in oocytes—especially double-strand breaks (DSBs), which occur naturally during the first meiosis phase (meiosis I) due to synaptic complex separation—affects the fertilization ability of oocytes, instead of causing cancer (as in somatic cells). The mechanism of oocytes to effectively repair DSB damage has not yet been clearly studied, especially considering medically induced DSBs superimposed on naturally occurring DSBs in meiosis I. It was found that maturation rates decreased or increased, respectively corresponding with overexpression or interference of p21 in bovine oocytes. At the same time, the maturation rate of bovine oocytes decreased with a gradual increase in Zeocin dose, and the p21 expression in those immature oocytes changed significantly with the gradual increase in Zeocin dose (same as increased DSB intensity). Same as p21, the variation trend of ATM expression was consistent with the gradual increase in Zeocin dose. Furthermore, the oocytes demonstrated tolerance to DSBs during meiosis I, while the maturation rates decreased when the damage exceeded a certain threshold; according to which, it may be that ATM regulates the p53–p21 pathway to affect the completion of meiosis. In addition, nonhomologous recombination and cumulus cells are potentially involved in the process by which oocytes respond to DSB damage.
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spelling pubmed-77277022020-12-11 Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes Wang, Lili Xu, Xiaolei Teng, Mingming Zhao, Guimin Lei, Anmin Int J Mol Sci Article As a common injury almost all cells face, DNA damage in oocytes—especially double-strand breaks (DSBs), which occur naturally during the first meiosis phase (meiosis I) due to synaptic complex separation—affects the fertilization ability of oocytes, instead of causing cancer (as in somatic cells). The mechanism of oocytes to effectively repair DSB damage has not yet been clearly studied, especially considering medically induced DSBs superimposed on naturally occurring DSBs in meiosis I. It was found that maturation rates decreased or increased, respectively corresponding with overexpression or interference of p21 in bovine oocytes. At the same time, the maturation rate of bovine oocytes decreased with a gradual increase in Zeocin dose, and the p21 expression in those immature oocytes changed significantly with the gradual increase in Zeocin dose (same as increased DSB intensity). Same as p21, the variation trend of ATM expression was consistent with the gradual increase in Zeocin dose. Furthermore, the oocytes demonstrated tolerance to DSBs during meiosis I, while the maturation rates decreased when the damage exceeded a certain threshold; according to which, it may be that ATM regulates the p53–p21 pathway to affect the completion of meiosis. In addition, nonhomologous recombination and cumulus cells are potentially involved in the process by which oocytes respond to DSB damage. MDPI 2020-11-24 /pmc/articles/PMC7727702/ /pubmed/33255251 http://dx.doi.org/10.3390/ijms21238892 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Lili
Xu, Xiaolei
Teng, Mingming
Zhao, Guimin
Lei, Anmin
Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes
title Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes
title_full Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes
title_fullStr Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes
title_full_unstemmed Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes
title_short Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes
title_sort coping with dna double-strand breaks via atm signaling pathway in bovine oocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727702/
https://www.ncbi.nlm.nih.gov/pubmed/33255251
http://dx.doi.org/10.3390/ijms21238892
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