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Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells

Non-viral gene delivery using exogenous microRNAs is a potential strategy for fighting cancers with poor prognosis and which lack specific therapies, such as triple-negative breast cancer (TNBC). Herein we report the synthesis of six nontoxic electrostatic polymeric nanocapsules (P1 to P6) for micro...

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Autores principales: Djafari, Jamila, Fernández-Lodeiro, Javier, Santos, Hugo M., Lorenzo, Julia, Rodriguez-Calado, Sergi, Bértolo, Emilia, Capelo-Martínez, José Luis, Lodeiro, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727712/
https://www.ncbi.nlm.nih.gov/pubmed/33255217
http://dx.doi.org/10.3390/ma13235309
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author Djafari, Jamila
Fernández-Lodeiro, Javier
Santos, Hugo M.
Lorenzo, Julia
Rodriguez-Calado, Sergi
Bértolo, Emilia
Capelo-Martínez, José Luis
Lodeiro, Carlos
author_facet Djafari, Jamila
Fernández-Lodeiro, Javier
Santos, Hugo M.
Lorenzo, Julia
Rodriguez-Calado, Sergi
Bértolo, Emilia
Capelo-Martínez, José Luis
Lodeiro, Carlos
author_sort Djafari, Jamila
collection PubMed
description Non-viral gene delivery using exogenous microRNAs is a potential strategy for fighting cancers with poor prognosis and which lack specific therapies, such as triple-negative breast cancer (TNBC). Herein we report the synthesis of six nontoxic electrostatic polymeric nanocapsules (P1 to P6) for microRNA delivery in TNBC cells. 1H Nuclear Magnetic Resonance (NMR) spectroscopy and Scanning Electron Microscopy (SEM) were used to characterize the nanopolyplexes, synthesized with Poly(L-Lysine) and hyaluronic acid (Ha). Studies on the activity of the ternary HA/PLI/miRNA-34 nanopolyplexes towards TNBC cell line MDA-MB-231 were conducted. The nanopolyplexes mediated intracellular restoration of tumor suppressor miR34a was evaluated by using Western blotting to quantify the expression level of the Bcl-2 protein. The results suggest that the P5, with a ratio PLI/Ha of 0.05, was the most promising for the delivery of miR-34a into TNBC cells; the P5 nanocapsules were able to reduce Bcl-2 expression at a protein level, and had an effect in the overall cell viability after 24 h treatment.
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spelling pubmed-77277122020-12-11 Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells Djafari, Jamila Fernández-Lodeiro, Javier Santos, Hugo M. Lorenzo, Julia Rodriguez-Calado, Sergi Bértolo, Emilia Capelo-Martínez, José Luis Lodeiro, Carlos Materials (Basel) Communication Non-viral gene delivery using exogenous microRNAs is a potential strategy for fighting cancers with poor prognosis and which lack specific therapies, such as triple-negative breast cancer (TNBC). Herein we report the synthesis of six nontoxic electrostatic polymeric nanocapsules (P1 to P6) for microRNA delivery in TNBC cells. 1H Nuclear Magnetic Resonance (NMR) spectroscopy and Scanning Electron Microscopy (SEM) were used to characterize the nanopolyplexes, synthesized with Poly(L-Lysine) and hyaluronic acid (Ha). Studies on the activity of the ternary HA/PLI/miRNA-34 nanopolyplexes towards TNBC cell line MDA-MB-231 were conducted. The nanopolyplexes mediated intracellular restoration of tumor suppressor miR34a was evaluated by using Western blotting to quantify the expression level of the Bcl-2 protein. The results suggest that the P5, with a ratio PLI/Ha of 0.05, was the most promising for the delivery of miR-34a into TNBC cells; the P5 nanocapsules were able to reduce Bcl-2 expression at a protein level, and had an effect in the overall cell viability after 24 h treatment. MDPI 2020-11-24 /pmc/articles/PMC7727712/ /pubmed/33255217 http://dx.doi.org/10.3390/ma13235309 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Djafari, Jamila
Fernández-Lodeiro, Javier
Santos, Hugo M.
Lorenzo, Julia
Rodriguez-Calado, Sergi
Bértolo, Emilia
Capelo-Martínez, José Luis
Lodeiro, Carlos
Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells
title Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells
title_full Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells
title_fullStr Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells
title_full_unstemmed Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells
title_short Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells
title_sort study and preparation of multifunctional poly(l-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727712/
https://www.ncbi.nlm.nih.gov/pubmed/33255217
http://dx.doi.org/10.3390/ma13235309
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