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Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique

Wide field Raman imaging using the integral field spectroscopy approach was used as a fast, one shot imaging method for the simultaneous collection of all spectra composing a Raman image. For the suppression of autofluorescence and background signals such as room light, shifted excitation Raman diff...

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Autores principales: Korinth, Florian, Schmälzlin, Elmar, Stiebing, Clara, Urrutia, Tanya, Micheva, Genoveva, Sandin, Christer, Müller, André, Maiwald, Martin, Sumpf, Bernd, Krafft, Christoph, Tränkle, Günther, Roth, Martin M., Popp, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727830/
https://www.ncbi.nlm.nih.gov/pubmed/33255459
http://dx.doi.org/10.3390/s20236723
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author Korinth, Florian
Schmälzlin, Elmar
Stiebing, Clara
Urrutia, Tanya
Micheva, Genoveva
Sandin, Christer
Müller, André
Maiwald, Martin
Sumpf, Bernd
Krafft, Christoph
Tränkle, Günther
Roth, Martin M.
Popp, Jürgen
author_facet Korinth, Florian
Schmälzlin, Elmar
Stiebing, Clara
Urrutia, Tanya
Micheva, Genoveva
Sandin, Christer
Müller, André
Maiwald, Martin
Sumpf, Bernd
Krafft, Christoph
Tränkle, Günther
Roth, Martin M.
Popp, Jürgen
author_sort Korinth, Florian
collection PubMed
description Wide field Raman imaging using the integral field spectroscopy approach was used as a fast, one shot imaging method for the simultaneous collection of all spectra composing a Raman image. For the suppression of autofluorescence and background signals such as room light, shifted excitation Raman difference spectroscopy (SERDS) was applied to remove background artifacts in Raman spectra. To reduce acquisition times in wide field SERDS imaging, we adapted the nod and shuffle technique from astrophysics and implemented it into a wide field SERDS imaging setup. In our adapted version, the nod corresponds to the change in excitation wavelength, whereas the shuffle corresponds to the shifting of charges up and down on a Charge-Coupled Device (CCD) chip synchronous to the change in excitation wavelength. We coupled this improved wide field SERDS imaging setup to diode lasers with 784.4/785.5 and 457.7/458.9 nm excitation and applied it to samples such as paracetamol and aspirin tablets, polystyrene and polymethyl methacrylate beads, as well as pork meat using multiple accumulations with acquisition times in the range of 50 to 200 ms. The results tackle two main challenges of SERDS imaging: gradual photobleaching changes the autofluorescence background, and multiple readouts of CCD detector prolong the acquisition time.
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spelling pubmed-77278302020-12-11 Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique Korinth, Florian Schmälzlin, Elmar Stiebing, Clara Urrutia, Tanya Micheva, Genoveva Sandin, Christer Müller, André Maiwald, Martin Sumpf, Bernd Krafft, Christoph Tränkle, Günther Roth, Martin M. Popp, Jürgen Sensors (Basel) Article Wide field Raman imaging using the integral field spectroscopy approach was used as a fast, one shot imaging method for the simultaneous collection of all spectra composing a Raman image. For the suppression of autofluorescence and background signals such as room light, shifted excitation Raman difference spectroscopy (SERDS) was applied to remove background artifacts in Raman spectra. To reduce acquisition times in wide field SERDS imaging, we adapted the nod and shuffle technique from astrophysics and implemented it into a wide field SERDS imaging setup. In our adapted version, the nod corresponds to the change in excitation wavelength, whereas the shuffle corresponds to the shifting of charges up and down on a Charge-Coupled Device (CCD) chip synchronous to the change in excitation wavelength. We coupled this improved wide field SERDS imaging setup to diode lasers with 784.4/785.5 and 457.7/458.9 nm excitation and applied it to samples such as paracetamol and aspirin tablets, polystyrene and polymethyl methacrylate beads, as well as pork meat using multiple accumulations with acquisition times in the range of 50 to 200 ms. The results tackle two main challenges of SERDS imaging: gradual photobleaching changes the autofluorescence background, and multiple readouts of CCD detector prolong the acquisition time. MDPI 2020-11-24 /pmc/articles/PMC7727830/ /pubmed/33255459 http://dx.doi.org/10.3390/s20236723 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Korinth, Florian
Schmälzlin, Elmar
Stiebing, Clara
Urrutia, Tanya
Micheva, Genoveva
Sandin, Christer
Müller, André
Maiwald, Martin
Sumpf, Bernd
Krafft, Christoph
Tränkle, Günther
Roth, Martin M.
Popp, Jürgen
Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique
title Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique
title_full Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique
title_fullStr Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique
title_full_unstemmed Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique
title_short Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique
title_sort wide field spectral imaging with shifted excitation raman difference spectroscopy using the nod and shuffle technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727830/
https://www.ncbi.nlm.nih.gov/pubmed/33255459
http://dx.doi.org/10.3390/s20236723
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