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An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru

The assignment of accurate species names is crucial, especially for those with confirmed agronomic potential such as highland papayas. The use of additional methodologies and data sets is recommended to establish well-supported boundaries among species of Vasconcellea. Accordingly, six chloroplast (...

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Autores principales: Tineo, Daniel, Bustamante, Danilo E., Calderon, Martha S., Mendoza, Jani E., Huaman, Eyner, Oliva, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728213/
https://www.ncbi.nlm.nih.gov/pubmed/33301452
http://dx.doi.org/10.1371/journal.pone.0242469
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author Tineo, Daniel
Bustamante, Danilo E.
Calderon, Martha S.
Mendoza, Jani E.
Huaman, Eyner
Oliva, Manuel
author_facet Tineo, Daniel
Bustamante, Danilo E.
Calderon, Martha S.
Mendoza, Jani E.
Huaman, Eyner
Oliva, Manuel
author_sort Tineo, Daniel
collection PubMed
description The assignment of accurate species names is crucial, especially for those with confirmed agronomic potential such as highland papayas. The use of additional methodologies and data sets is recommended to establish well-supported boundaries among species of Vasconcellea. Accordingly, six chloroplast (trnL-trnF, rpl20-rps12, psbA-trnH intergenic spacers, matK and rbcL genes) and nuclear (ITS) markers were used to delimit species in the genus Vasconcellea using phylogeny and four DNA-based methods. Our results demonstrated congruence among different methodologies applied in this integrative study (i.e., morphology, multilocus phylogeny, genetic distance, coalescence methods). Genetic distance (ABGD, SPN), a coalescence method (BPP), and the multilocus phylogeny supported 22–25 different species of Vasconcellea, including the following five new species from northern Peru: V. badilloi sp. nov., V. carvalhoae sp. nov., V. chachapoyensis sp. nov., V. pentalobis sp. nov., and V. peruviensis sp. nov. Genetic markers that gave better resolution for distinguishing species were ITS and trnL-trnF. Phylogenetic diversity and DNA-species delimitation methods could be used to discover taxa within traditionally defined species.
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spelling pubmed-77282132020-12-16 An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru Tineo, Daniel Bustamante, Danilo E. Calderon, Martha S. Mendoza, Jani E. Huaman, Eyner Oliva, Manuel PLoS One Research Article The assignment of accurate species names is crucial, especially for those with confirmed agronomic potential such as highland papayas. The use of additional methodologies and data sets is recommended to establish well-supported boundaries among species of Vasconcellea. Accordingly, six chloroplast (trnL-trnF, rpl20-rps12, psbA-trnH intergenic spacers, matK and rbcL genes) and nuclear (ITS) markers were used to delimit species in the genus Vasconcellea using phylogeny and four DNA-based methods. Our results demonstrated congruence among different methodologies applied in this integrative study (i.e., morphology, multilocus phylogeny, genetic distance, coalescence methods). Genetic distance (ABGD, SPN), a coalescence method (BPP), and the multilocus phylogeny supported 22–25 different species of Vasconcellea, including the following five new species from northern Peru: V. badilloi sp. nov., V. carvalhoae sp. nov., V. chachapoyensis sp. nov., V. pentalobis sp. nov., and V. peruviensis sp. nov. Genetic markers that gave better resolution for distinguishing species were ITS and trnL-trnF. Phylogenetic diversity and DNA-species delimitation methods could be used to discover taxa within traditionally defined species. Public Library of Science 2020-12-10 /pmc/articles/PMC7728213/ /pubmed/33301452 http://dx.doi.org/10.1371/journal.pone.0242469 Text en © 2020 Tineo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tineo, Daniel
Bustamante, Danilo E.
Calderon, Martha S.
Mendoza, Jani E.
Huaman, Eyner
Oliva, Manuel
An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru
title An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru
title_full An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru
title_fullStr An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru
title_full_unstemmed An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru
title_short An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru
title_sort integrative approach reveals five new species of highland papayas (caricaceae, vasconcellea) from northern peru
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728213/
https://www.ncbi.nlm.nih.gov/pubmed/33301452
http://dx.doi.org/10.1371/journal.pone.0242469
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