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An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru
The assignment of accurate species names is crucial, especially for those with confirmed agronomic potential such as highland papayas. The use of additional methodologies and data sets is recommended to establish well-supported boundaries among species of Vasconcellea. Accordingly, six chloroplast (...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728213/ https://www.ncbi.nlm.nih.gov/pubmed/33301452 http://dx.doi.org/10.1371/journal.pone.0242469 |
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author | Tineo, Daniel Bustamante, Danilo E. Calderon, Martha S. Mendoza, Jani E. Huaman, Eyner Oliva, Manuel |
author_facet | Tineo, Daniel Bustamante, Danilo E. Calderon, Martha S. Mendoza, Jani E. Huaman, Eyner Oliva, Manuel |
author_sort | Tineo, Daniel |
collection | PubMed |
description | The assignment of accurate species names is crucial, especially for those with confirmed agronomic potential such as highland papayas. The use of additional methodologies and data sets is recommended to establish well-supported boundaries among species of Vasconcellea. Accordingly, six chloroplast (trnL-trnF, rpl20-rps12, psbA-trnH intergenic spacers, matK and rbcL genes) and nuclear (ITS) markers were used to delimit species in the genus Vasconcellea using phylogeny and four DNA-based methods. Our results demonstrated congruence among different methodologies applied in this integrative study (i.e., morphology, multilocus phylogeny, genetic distance, coalescence methods). Genetic distance (ABGD, SPN), a coalescence method (BPP), and the multilocus phylogeny supported 22–25 different species of Vasconcellea, including the following five new species from northern Peru: V. badilloi sp. nov., V. carvalhoae sp. nov., V. chachapoyensis sp. nov., V. pentalobis sp. nov., and V. peruviensis sp. nov. Genetic markers that gave better resolution for distinguishing species were ITS and trnL-trnF. Phylogenetic diversity and DNA-species delimitation methods could be used to discover taxa within traditionally defined species. |
format | Online Article Text |
id | pubmed-7728213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-77282132020-12-16 An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru Tineo, Daniel Bustamante, Danilo E. Calderon, Martha S. Mendoza, Jani E. Huaman, Eyner Oliva, Manuel PLoS One Research Article The assignment of accurate species names is crucial, especially for those with confirmed agronomic potential such as highland papayas. The use of additional methodologies and data sets is recommended to establish well-supported boundaries among species of Vasconcellea. Accordingly, six chloroplast (trnL-trnF, rpl20-rps12, psbA-trnH intergenic spacers, matK and rbcL genes) and nuclear (ITS) markers were used to delimit species in the genus Vasconcellea using phylogeny and four DNA-based methods. Our results demonstrated congruence among different methodologies applied in this integrative study (i.e., morphology, multilocus phylogeny, genetic distance, coalescence methods). Genetic distance (ABGD, SPN), a coalescence method (BPP), and the multilocus phylogeny supported 22–25 different species of Vasconcellea, including the following five new species from northern Peru: V. badilloi sp. nov., V. carvalhoae sp. nov., V. chachapoyensis sp. nov., V. pentalobis sp. nov., and V. peruviensis sp. nov. Genetic markers that gave better resolution for distinguishing species were ITS and trnL-trnF. Phylogenetic diversity and DNA-species delimitation methods could be used to discover taxa within traditionally defined species. Public Library of Science 2020-12-10 /pmc/articles/PMC7728213/ /pubmed/33301452 http://dx.doi.org/10.1371/journal.pone.0242469 Text en © 2020 Tineo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Tineo, Daniel Bustamante, Danilo E. Calderon, Martha S. Mendoza, Jani E. Huaman, Eyner Oliva, Manuel An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru |
title | An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru |
title_full | An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru |
title_fullStr | An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru |
title_full_unstemmed | An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru |
title_short | An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru |
title_sort | integrative approach reveals five new species of highland papayas (caricaceae, vasconcellea) from northern peru |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728213/ https://www.ncbi.nlm.nih.gov/pubmed/33301452 http://dx.doi.org/10.1371/journal.pone.0242469 |
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