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Serotonin 2A (5-HT(2A)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells

5-HT(2A,) a G-protein coupled receptor, is widely expressed in the human body, including in the gastrointestinal tract, platelets and the nervous system. It mediates various functions, for e.g. learning, memory, mood regulation, platelet aggregation and vasoconstriction, but its involvement in cell-...

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Autores principales: John Jayakumar, Joe Anand Kumar, Panicker, Mitradas. M., Basu, Basudha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728786/
https://www.ncbi.nlm.nih.gov/pubmed/33303826
http://dx.doi.org/10.1038/s41598-020-78595-6
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author John Jayakumar, Joe Anand Kumar
Panicker, Mitradas. M.
Basu, Basudha
author_facet John Jayakumar, Joe Anand Kumar
Panicker, Mitradas. M.
Basu, Basudha
author_sort John Jayakumar, Joe Anand Kumar
collection PubMed
description 5-HT(2A,) a G-protein coupled receptor, is widely expressed in the human body, including in the gastrointestinal tract, platelets and the nervous system. It mediates various functions, for e.g. learning, memory, mood regulation, platelet aggregation and vasoconstriction, but its involvement in cell-adhesion remains largely unknown. Here we report a novel role for 5-HT(2A) in cell–matrix adhesion. In HEK293 cells, which are loosely adherent, expression and stimulation of human or rat 5-HT(2A) receptor by agonists such as serotonin or 2,5-dimethoxy-4-iodoamphetamine (DOI) led to a significant increase in adhesion, while inhibition of 5-HT(2A) by antipsychotics, such as risperidone, olanzapine or chlorpromazine prevented it. 5-HT(2A) activation gave rise to stress fibers in these cells and was also required for their maintenance. Mechanistically, the 5-HT(2A)-mediated adhesion was mediated by downstream PKC and Rho signaling. Since 5-HT(2A) is associated with many disorders such as dementia, depression and schizophrenia, its role in cell–matrix adhesion could have implications for neural circuits.
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spelling pubmed-77287862020-12-14 Serotonin 2A (5-HT(2A)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells John Jayakumar, Joe Anand Kumar Panicker, Mitradas. M. Basu, Basudha Sci Rep Article 5-HT(2A,) a G-protein coupled receptor, is widely expressed in the human body, including in the gastrointestinal tract, platelets and the nervous system. It mediates various functions, for e.g. learning, memory, mood regulation, platelet aggregation and vasoconstriction, but its involvement in cell-adhesion remains largely unknown. Here we report a novel role for 5-HT(2A) in cell–matrix adhesion. In HEK293 cells, which are loosely adherent, expression and stimulation of human or rat 5-HT(2A) receptor by agonists such as serotonin or 2,5-dimethoxy-4-iodoamphetamine (DOI) led to a significant increase in adhesion, while inhibition of 5-HT(2A) by antipsychotics, such as risperidone, olanzapine or chlorpromazine prevented it. 5-HT(2A) activation gave rise to stress fibers in these cells and was also required for their maintenance. Mechanistically, the 5-HT(2A)-mediated adhesion was mediated by downstream PKC and Rho signaling. Since 5-HT(2A) is associated with many disorders such as dementia, depression and schizophrenia, its role in cell–matrix adhesion could have implications for neural circuits. Nature Publishing Group UK 2020-12-10 /pmc/articles/PMC7728786/ /pubmed/33303826 http://dx.doi.org/10.1038/s41598-020-78595-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
John Jayakumar, Joe Anand Kumar
Panicker, Mitradas. M.
Basu, Basudha
Serotonin 2A (5-HT(2A)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title Serotonin 2A (5-HT(2A)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_full Serotonin 2A (5-HT(2A)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_fullStr Serotonin 2A (5-HT(2A)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_full_unstemmed Serotonin 2A (5-HT(2A)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_short Serotonin 2A (5-HT(2A)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells
title_sort serotonin 2a (5-ht(2a)) receptor affects cell–matrix adhesion and the formation and maintenance of stress fibers in hek293 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728786/
https://www.ncbi.nlm.nih.gov/pubmed/33303826
http://dx.doi.org/10.1038/s41598-020-78595-6
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