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Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves
Ginkgo (Ginkgo biloba L.) is a high-value medicinal tree species characterized by its flavonoids beneficial effects that are abundant in leaves. We performed a temporospatial comprehensive transcriptome and metabolome dynamics analyses of clonally propagated Ginkgo plants at four developmental stage...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728922/ https://www.ncbi.nlm.nih.gov/pubmed/33329734 http://dx.doi.org/10.3389/fgene.2020.589326 |
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author | Guo, Ying Wang, Tongli Fu, Fang-Fang El-Kassaby, Yousry A. Wang, Guibin |
author_facet | Guo, Ying Wang, Tongli Fu, Fang-Fang El-Kassaby, Yousry A. Wang, Guibin |
author_sort | Guo, Ying |
collection | PubMed |
description | Ginkgo (Ginkgo biloba L.) is a high-value medicinal tree species characterized by its flavonoids beneficial effects that are abundant in leaves. We performed a temporospatial comprehensive transcriptome and metabolome dynamics analyses of clonally propagated Ginkgo plants at four developmental stages (time: May to August) across three different environments (space) to unravel leaves flavonoids biosynthesis variation. Principal component analysis revealed clear gene expression separation across samples from different environments and leaf-developmental stages. We found that flavonoid-related metabolism was more active in the early stage of leaf development, and the content of total flavonoid glycosides and the expression of some genes in flavonoid biosynthesis pathway peaked in May. We also constructed a co-expression regulation network and identified eight GbMYBs and combining with other TF genes (3 GbERFs, 1 GbbHLH, and 1 GbTrihelix) positively regulated the expression of multiple structural genes in the flavonoid biosynthesis pathway. We found that part of these GbTFs (Gb_11316, Gb_32143, and Gb_00128) expressions was negatively correlated with mean minimum temperature and mean relative humidity, while positively correlated with sunshine duration. This study increased our understanding of the molecular mechanisms of flavonoids biosynthesis in Ginkgo leaves and provided insight into the proper production and management of Ginkgo commercial plantations. |
format | Online Article Text |
id | pubmed-7728922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77289222020-12-15 Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves Guo, Ying Wang, Tongli Fu, Fang-Fang El-Kassaby, Yousry A. Wang, Guibin Front Genet Genetics Ginkgo (Ginkgo biloba L.) is a high-value medicinal tree species characterized by its flavonoids beneficial effects that are abundant in leaves. We performed a temporospatial comprehensive transcriptome and metabolome dynamics analyses of clonally propagated Ginkgo plants at four developmental stages (time: May to August) across three different environments (space) to unravel leaves flavonoids biosynthesis variation. Principal component analysis revealed clear gene expression separation across samples from different environments and leaf-developmental stages. We found that flavonoid-related metabolism was more active in the early stage of leaf development, and the content of total flavonoid glycosides and the expression of some genes in flavonoid biosynthesis pathway peaked in May. We also constructed a co-expression regulation network and identified eight GbMYBs and combining with other TF genes (3 GbERFs, 1 GbbHLH, and 1 GbTrihelix) positively regulated the expression of multiple structural genes in the flavonoid biosynthesis pathway. We found that part of these GbTFs (Gb_11316, Gb_32143, and Gb_00128) expressions was negatively correlated with mean minimum temperature and mean relative humidity, while positively correlated with sunshine duration. This study increased our understanding of the molecular mechanisms of flavonoids biosynthesis in Ginkgo leaves and provided insight into the proper production and management of Ginkgo commercial plantations. Frontiers Media S.A. 2020-11-27 /pmc/articles/PMC7728922/ /pubmed/33329734 http://dx.doi.org/10.3389/fgene.2020.589326 Text en Copyright © 2020 Guo, Wang, Fu, El-Kassaby and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Guo, Ying Wang, Tongli Fu, Fang-Fang El-Kassaby, Yousry A. Wang, Guibin Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves |
title | Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves |
title_full | Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves |
title_fullStr | Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves |
title_full_unstemmed | Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves |
title_short | Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves |
title_sort | temporospatial flavonoids metabolism variation in ginkgo biloba leaves |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728922/ https://www.ncbi.nlm.nih.gov/pubmed/33329734 http://dx.doi.org/10.3389/fgene.2020.589326 |
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