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The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology

There is increasing emphasis on the use of new analytical approaches in subject analysis and classification, particularly in respect to minimal sample preparation. Here, we demonstrate that rapid evaporative ionization mass spectrometry (REIMS), a method that captures metabolite mass spectra after r...

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Autores principales: Wagner, Iris, Koch, Natalie I., Sarsby, Joscelyn, White, Nicola, Price, Tom A. R., Jones, Sam, Hurst, Jane L., Beynon, Robert J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729031/
https://www.ncbi.nlm.nih.gov/pubmed/33234068
http://dx.doi.org/10.1098/rsob.200196
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author Wagner, Iris
Koch, Natalie I.
Sarsby, Joscelyn
White, Nicola
Price, Tom A. R.
Jones, Sam
Hurst, Jane L.
Beynon, Robert J.
author_facet Wagner, Iris
Koch, Natalie I.
Sarsby, Joscelyn
White, Nicola
Price, Tom A. R.
Jones, Sam
Hurst, Jane L.
Beynon, Robert J.
author_sort Wagner, Iris
collection PubMed
description There is increasing emphasis on the use of new analytical approaches in subject analysis and classification, particularly in respect to minimal sample preparation. Here, we demonstrate that rapid evaporative ionization mass spectrometry (REIMS), a method that captures metabolite mass spectra after rapid combustive degradation of an intact biological specimen, generates informative mass spectra from several arthropods, and more specifically, is capable of discerning differences between species and sex of several adult Drosophila species. A model including five Drosophila species, built using pattern recognition, achieves high correct classification rates (over 90%) using test datasets and is able to resolve closely related species. The ease of discrimination of male and female specimens also demonstrates that sex-specific differences reside in the REIMS metabolite patterns, whether analysed across all five species or specifically for D. melanogaster. Further, the same approach can correctly discriminate and assign Drosophila species at the larval stage, where these are morphologically highly similar or identical. REIMS offers a novel approach to insect typing and analysis, requiring a few seconds of data acquisition per sample and has considerable potential as a new tool for the field biologist.
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spelling pubmed-77290312020-12-11 The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology Wagner, Iris Koch, Natalie I. Sarsby, Joscelyn White, Nicola Price, Tom A. R. Jones, Sam Hurst, Jane L. Beynon, Robert J. Open Biol Research There is increasing emphasis on the use of new analytical approaches in subject analysis and classification, particularly in respect to minimal sample preparation. Here, we demonstrate that rapid evaporative ionization mass spectrometry (REIMS), a method that captures metabolite mass spectra after rapid combustive degradation of an intact biological specimen, generates informative mass spectra from several arthropods, and more specifically, is capable of discerning differences between species and sex of several adult Drosophila species. A model including five Drosophila species, built using pattern recognition, achieves high correct classification rates (over 90%) using test datasets and is able to resolve closely related species. The ease of discrimination of male and female specimens also demonstrates that sex-specific differences reside in the REIMS metabolite patterns, whether analysed across all five species or specifically for D. melanogaster. Further, the same approach can correctly discriminate and assign Drosophila species at the larval stage, where these are morphologically highly similar or identical. REIMS offers a novel approach to insect typing and analysis, requiring a few seconds of data acquisition per sample and has considerable potential as a new tool for the field biologist. The Royal Society 2020-11-25 /pmc/articles/PMC7729031/ /pubmed/33234068 http://dx.doi.org/10.1098/rsob.200196 Text en © 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research
Wagner, Iris
Koch, Natalie I.
Sarsby, Joscelyn
White, Nicola
Price, Tom A. R.
Jones, Sam
Hurst, Jane L.
Beynon, Robert J.
The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology
title The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology
title_full The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology
title_fullStr The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology
title_full_unstemmed The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology
title_short The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology
title_sort application of rapid evaporative ionization mass spectrometry in the analysis of drosophila species—a potential new tool in entomology
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729031/
https://www.ncbi.nlm.nih.gov/pubmed/33234068
http://dx.doi.org/10.1098/rsob.200196
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