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Plasma Soluble CD146 as a Potential Diagnostic Marker of Acute Rejection in Kidney Transplantation
Previous studies have implicated the role of CD146 and its soluble form (sCD146) in the pathogenesis of inflammatory diseases. However, the association between CD146 and acute rejection in kidney transplant patients remains unexplored. In this study, fifty-six patients with biopsy-proved rejection o...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729194/ https://www.ncbi.nlm.nih.gov/pubmed/33330520 http://dx.doi.org/10.3389/fmed.2020.531999 |
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author | Liao, Jun Fu, Qian Chen, Wenfang Li, Jun Zhang, Wenhui Zhang, Huanxi Gao, Yifang Yang, Shicong Xu, Bowen Huang, Huiting Wang, Jiali Li, Xirui Liu, Longshan Wang, Changxi |
author_facet | Liao, Jun Fu, Qian Chen, Wenfang Li, Jun Zhang, Wenhui Zhang, Huanxi Gao, Yifang Yang, Shicong Xu, Bowen Huang, Huiting Wang, Jiali Li, Xirui Liu, Longshan Wang, Changxi |
author_sort | Liao, Jun |
collection | PubMed |
description | Previous studies have implicated the role of CD146 and its soluble form (sCD146) in the pathogenesis of inflammatory diseases. However, the association between CD146 and acute rejection in kidney transplant patients remains unexplored. In this study, fifty-six patients with biopsy-proved rejection or non-rejection and 11 stable allograft function patients were retrospectively analyzed. Soluble CD146 in plasma was detected in peripheral blood by enzyme linked immunosorbent assay (ELISA), and local CD146 expression in graft biopsy was detected by immunohistochemistry. We found that plasma soluble CD146 in acute rejection recipients was significantly higher than in stable patients without rejection, and the biopsy CD146 staining in the rejection group was higher than that of the non-rejection group. Multivariate analysis demonstrated soluble CD146 as an independent risk factor of acute rejection. The area under the receiver operating characteristic curve (AUC) of sCD146 for AR diagnosis was 0.895, and the optimal cut-off value was 75.64 ng/ml, with a sensitivity of 87.8% and a specificity of 80.8%, which was better than eGFR alone (P = 0.02496). Immunohistochemistry showed CD146 expression in glomeruli was positively correlated with the Banff-g score, and its expression in tubules also had a positive relationship with the Banff-t score. Therefore, soluble CD146 may be a potential biomarker of acute rejection. Increased CD146 expression in the endothelial or tubular epithelial cells may imply that endothelial/epithelial dysfunction is involved in the pathogenesis of immune injury. |
format | Online Article Text |
id | pubmed-7729194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77291942020-12-15 Plasma Soluble CD146 as a Potential Diagnostic Marker of Acute Rejection in Kidney Transplantation Liao, Jun Fu, Qian Chen, Wenfang Li, Jun Zhang, Wenhui Zhang, Huanxi Gao, Yifang Yang, Shicong Xu, Bowen Huang, Huiting Wang, Jiali Li, Xirui Liu, Longshan Wang, Changxi Front Med (Lausanne) Medicine Previous studies have implicated the role of CD146 and its soluble form (sCD146) in the pathogenesis of inflammatory diseases. However, the association between CD146 and acute rejection in kidney transplant patients remains unexplored. In this study, fifty-six patients with biopsy-proved rejection or non-rejection and 11 stable allograft function patients were retrospectively analyzed. Soluble CD146 in plasma was detected in peripheral blood by enzyme linked immunosorbent assay (ELISA), and local CD146 expression in graft biopsy was detected by immunohistochemistry. We found that plasma soluble CD146 in acute rejection recipients was significantly higher than in stable patients without rejection, and the biopsy CD146 staining in the rejection group was higher than that of the non-rejection group. Multivariate analysis demonstrated soluble CD146 as an independent risk factor of acute rejection. The area under the receiver operating characteristic curve (AUC) of sCD146 for AR diagnosis was 0.895, and the optimal cut-off value was 75.64 ng/ml, with a sensitivity of 87.8% and a specificity of 80.8%, which was better than eGFR alone (P = 0.02496). Immunohistochemistry showed CD146 expression in glomeruli was positively correlated with the Banff-g score, and its expression in tubules also had a positive relationship with the Banff-t score. Therefore, soluble CD146 may be a potential biomarker of acute rejection. Increased CD146 expression in the endothelial or tubular epithelial cells may imply that endothelial/epithelial dysfunction is involved in the pathogenesis of immune injury. Frontiers Media S.A. 2020-11-25 /pmc/articles/PMC7729194/ /pubmed/33330520 http://dx.doi.org/10.3389/fmed.2020.531999 Text en Copyright © 2020 Liao, Fu, Chen, Li, Zhang, Zhang, Gao, Yang, Xu, Huang, Wang, Li, Liu and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Liao, Jun Fu, Qian Chen, Wenfang Li, Jun Zhang, Wenhui Zhang, Huanxi Gao, Yifang Yang, Shicong Xu, Bowen Huang, Huiting Wang, Jiali Li, Xirui Liu, Longshan Wang, Changxi Plasma Soluble CD146 as a Potential Diagnostic Marker of Acute Rejection in Kidney Transplantation |
title | Plasma Soluble CD146 as a Potential Diagnostic Marker of Acute Rejection in Kidney Transplantation |
title_full | Plasma Soluble CD146 as a Potential Diagnostic Marker of Acute Rejection in Kidney Transplantation |
title_fullStr | Plasma Soluble CD146 as a Potential Diagnostic Marker of Acute Rejection in Kidney Transplantation |
title_full_unstemmed | Plasma Soluble CD146 as a Potential Diagnostic Marker of Acute Rejection in Kidney Transplantation |
title_short | Plasma Soluble CD146 as a Potential Diagnostic Marker of Acute Rejection in Kidney Transplantation |
title_sort | plasma soluble cd146 as a potential diagnostic marker of acute rejection in kidney transplantation |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729194/ https://www.ncbi.nlm.nih.gov/pubmed/33330520 http://dx.doi.org/10.3389/fmed.2020.531999 |
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