Strand Displacement Activity of PrimPol

Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn(2+) ions and accessory replicative proteins RPA and PolDIP2....

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Detalles Bibliográficos
Autores principales: Boldinova, Elizaveta O., Belousova, Ekaterina A., Gagarinskaya, Diana I., Maltseva, Ekaterina A., Khodyreva, Svetlana N., Lavrik, Olga I., Makarova, Alena V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729601/
https://www.ncbi.nlm.nih.gov/pubmed/33261049
http://dx.doi.org/10.3390/ijms21239027
Descripción
Sumario:Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn(2+) ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5′-DNA flaps generated by PrimPol in vitro.

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