Strand Displacement Activity of PrimPol

Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn(2+) ions and accessory replicative proteins RPA and PolDIP2....

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Autores principales: Boldinova, Elizaveta O., Belousova, Ekaterina A., Gagarinskaya, Diana I., Maltseva, Ekaterina A., Khodyreva, Svetlana N., Lavrik, Olga I., Makarova, Alena V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729601/
https://www.ncbi.nlm.nih.gov/pubmed/33261049
http://dx.doi.org/10.3390/ijms21239027
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author Boldinova, Elizaveta O.
Belousova, Ekaterina A.
Gagarinskaya, Diana I.
Maltseva, Ekaterina A.
Khodyreva, Svetlana N.
Lavrik, Olga I.
Makarova, Alena V.
author_facet Boldinova, Elizaveta O.
Belousova, Ekaterina A.
Gagarinskaya, Diana I.
Maltseva, Ekaterina A.
Khodyreva, Svetlana N.
Lavrik, Olga I.
Makarova, Alena V.
author_sort Boldinova, Elizaveta O.
collection PubMed
description Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn(2+) ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5′-DNA flaps generated by PrimPol in vitro.
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spelling pubmed-77296012020-12-12 Strand Displacement Activity of PrimPol Boldinova, Elizaveta O. Belousova, Ekaterina A. Gagarinskaya, Diana I. Maltseva, Ekaterina A. Khodyreva, Svetlana N. Lavrik, Olga I. Makarova, Alena V. Int J Mol Sci Article Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn(2+) ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5′-DNA flaps generated by PrimPol in vitro. MDPI 2020-11-27 /pmc/articles/PMC7729601/ /pubmed/33261049 http://dx.doi.org/10.3390/ijms21239027 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boldinova, Elizaveta O.
Belousova, Ekaterina A.
Gagarinskaya, Diana I.
Maltseva, Ekaterina A.
Khodyreva, Svetlana N.
Lavrik, Olga I.
Makarova, Alena V.
Strand Displacement Activity of PrimPol
title Strand Displacement Activity of PrimPol
title_full Strand Displacement Activity of PrimPol
title_fullStr Strand Displacement Activity of PrimPol
title_full_unstemmed Strand Displacement Activity of PrimPol
title_short Strand Displacement Activity of PrimPol
title_sort strand displacement activity of primpol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729601/
https://www.ncbi.nlm.nih.gov/pubmed/33261049
http://dx.doi.org/10.3390/ijms21239027
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AT khodyrevasvetlanan stranddisplacementactivityofprimpol
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