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Expression and role of ABIN1 in sepsis: In vitro and in vivo studies

In this research, we attempted to explain the effect and the related molecular mechanisms of ABIN1 in lipopolysaccharide (LPS)-induced septic mice or RAW264.7 macrophages. LPS was adopted to treat RAW264.7 macrophages for 4 h, and the levels of inflammatory factors were assessed by ELISA. Besides, A...

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Autores principales: Li, Haolan, Sun, Aichen, Meng, Taocheng, Zhu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729633/
https://www.ncbi.nlm.nih.gov/pubmed/33364432
http://dx.doi.org/10.1515/med-2021-0008
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author Li, Haolan
Sun, Aichen
Meng, Taocheng
Zhu, Yan
author_facet Li, Haolan
Sun, Aichen
Meng, Taocheng
Zhu, Yan
author_sort Li, Haolan
collection PubMed
description In this research, we attempted to explain the effect and the related molecular mechanisms of ABIN1 in lipopolysaccharide (LPS)-induced septic mice or RAW264.7 macrophages. LPS was adopted to treat RAW264.7 macrophages for 4 h, and the levels of inflammatory factors were assessed by ELISA. Besides, ABIN1 expression was measured by quantitative reverse transcription polymerase chain reaction. Apparently, LPS enhanced immunoreaction, suggested by increased expression of IL-1β, tumor necrosis factor (TNF)-α, and IL-6. ABIN1 levels were obviously reduced compared to the control. Furthermore, we evaluated the roles of ABIN1-plasmid in immunoreaction and nuclear factor-κB (NF-κB) pathway. We found that ABIN1-plasmid significantly reduced the expression of IL-1β, TNF-α, and IL-6 in LPS-treated cells and inhibited NF-κB pathway activation. Meanwhile, a septic mouse mode was conducted to validate the role of ABIN1 in inflammatory response and organ damage in vivo. These data suggested that ABIN1-plasmid significantly inhibited the secretion of inflammatory cytokines and Cr, BUN, AST, and ALT levels in the serum of LPS-stimulated mice compared to LPS + control-plasmid group, reflecting the relieved inflammation and organ injury. In summary, the present findings indicated that ABIN1 alleviated sepsis by repressing inflammatory response through NF-κB signaling pathway, emphasizing the potential value of ABIN1 as therapeutic strategy for sepsis.
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spelling pubmed-77296332020-12-23 Expression and role of ABIN1 in sepsis: In vitro and in vivo studies Li, Haolan Sun, Aichen Meng, Taocheng Zhu, Yan Open Med (Wars) Research Article In this research, we attempted to explain the effect and the related molecular mechanisms of ABIN1 in lipopolysaccharide (LPS)-induced septic mice or RAW264.7 macrophages. LPS was adopted to treat RAW264.7 macrophages for 4 h, and the levels of inflammatory factors were assessed by ELISA. Besides, ABIN1 expression was measured by quantitative reverse transcription polymerase chain reaction. Apparently, LPS enhanced immunoreaction, suggested by increased expression of IL-1β, tumor necrosis factor (TNF)-α, and IL-6. ABIN1 levels were obviously reduced compared to the control. Furthermore, we evaluated the roles of ABIN1-plasmid in immunoreaction and nuclear factor-κB (NF-κB) pathway. We found that ABIN1-plasmid significantly reduced the expression of IL-1β, TNF-α, and IL-6 in LPS-treated cells and inhibited NF-κB pathway activation. Meanwhile, a septic mouse mode was conducted to validate the role of ABIN1 in inflammatory response and organ damage in vivo. These data suggested that ABIN1-plasmid significantly inhibited the secretion of inflammatory cytokines and Cr, BUN, AST, and ALT levels in the serum of LPS-stimulated mice compared to LPS + control-plasmid group, reflecting the relieved inflammation and organ injury. In summary, the present findings indicated that ABIN1 alleviated sepsis by repressing inflammatory response through NF-κB signaling pathway, emphasizing the potential value of ABIN1 as therapeutic strategy for sepsis. De Gruyter 2020-12-04 /pmc/articles/PMC7729633/ /pubmed/33364432 http://dx.doi.org/10.1515/med-2021-0008 Text en © 2021 Haolan Li et al., published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Li, Haolan
Sun, Aichen
Meng, Taocheng
Zhu, Yan
Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
title Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
title_full Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
title_fullStr Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
title_full_unstemmed Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
title_short Expression and role of ABIN1 in sepsis: In vitro and in vivo studies
title_sort expression and role of abin1 in sepsis: in vitro and in vivo studies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729633/
https://www.ncbi.nlm.nih.gov/pubmed/33364432
http://dx.doi.org/10.1515/med-2021-0008
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