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Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS(n)

Astragali Radix total flavonoids (ARTF) is one of the main bioactive components of Astragali Radix (AR), and has many pharmacological effects. However, its metabolism and effective forms remains unclear. The HPLC-DAD-ESI-IT-TOF-MS(n) technique was used to screen and tentatively identify the in vivo...

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Autores principales: Liu, Li-Jia, Li, Hong-Fu, Xu, Feng, Wang, Hong-Yan, Zhang, Yi-Fan, Liu, Guang-Xue, Shang, Ming-Ying, Wang, Xuan, Cai, Shao-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729672/
https://www.ncbi.nlm.nih.gov/pubmed/33256251
http://dx.doi.org/10.3390/molecules25235560
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author Liu, Li-Jia
Li, Hong-Fu
Xu, Feng
Wang, Hong-Yan
Zhang, Yi-Fan
Liu, Guang-Xue
Shang, Ming-Ying
Wang, Xuan
Cai, Shao-Qing
author_facet Liu, Li-Jia
Li, Hong-Fu
Xu, Feng
Wang, Hong-Yan
Zhang, Yi-Fan
Liu, Guang-Xue
Shang, Ming-Ying
Wang, Xuan
Cai, Shao-Qing
author_sort Liu, Li-Jia
collection PubMed
description Astragali Radix total flavonoids (ARTF) is one of the main bioactive components of Astragali Radix (AR), and has many pharmacological effects. However, its metabolism and effective forms remains unclear. The HPLC-DAD-ESI-IT-TOF-MS(n) technique was used to screen and tentatively identify the in vivo original constituents and metabolites of ARTF and to clarify their distribution in rats after oral administration. In addition, modern chromatographic methods were used to isolate the main metabolites from rat urine and NMR spectroscopy was used to elucidate their structures. As a result, 170 compounds (23 original constituents and 147 metabolites) were tentatively identified as forms existing in vivo, 13 of which have the same pharmacological effect with ARTF. Among 170 compounds, three were newly detected original constituents in vivo and 89 were new metabolites of ARTF, from which 12 metabolites were regarded as new compounds. Nineteen original constituents and 65 metabolites were detected in 10 organs. Four metabolites were isolated and identified from rat urine, including a new compound (calycoisn-3’-O-glucuronide methyl ester), a firstly-isolated metabolite (astraisoflavan-7-O-glucoside-2’-O-glucuronide), and two known metabolites (daidzein-7-O-sulfate and calycosin-3’-O-glucuronide). The original constituents and metabolites existing in vivo may be material basis for ARTF efficacy, and these findings are helpful for further clarifying the effective forms of ARTF.
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spelling pubmed-77296722020-12-12 Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS(n) Liu, Li-Jia Li, Hong-Fu Xu, Feng Wang, Hong-Yan Zhang, Yi-Fan Liu, Guang-Xue Shang, Ming-Ying Wang, Xuan Cai, Shao-Qing Molecules Article Astragali Radix total flavonoids (ARTF) is one of the main bioactive components of Astragali Radix (AR), and has many pharmacological effects. However, its metabolism and effective forms remains unclear. The HPLC-DAD-ESI-IT-TOF-MS(n) technique was used to screen and tentatively identify the in vivo original constituents and metabolites of ARTF and to clarify their distribution in rats after oral administration. In addition, modern chromatographic methods were used to isolate the main metabolites from rat urine and NMR spectroscopy was used to elucidate their structures. As a result, 170 compounds (23 original constituents and 147 metabolites) were tentatively identified as forms existing in vivo, 13 of which have the same pharmacological effect with ARTF. Among 170 compounds, three were newly detected original constituents in vivo and 89 were new metabolites of ARTF, from which 12 metabolites were regarded as new compounds. Nineteen original constituents and 65 metabolites were detected in 10 organs. Four metabolites were isolated and identified from rat urine, including a new compound (calycoisn-3’-O-glucuronide methyl ester), a firstly-isolated metabolite (astraisoflavan-7-O-glucoside-2’-O-glucuronide), and two known metabolites (daidzein-7-O-sulfate and calycosin-3’-O-glucuronide). The original constituents and metabolites existing in vivo may be material basis for ARTF efficacy, and these findings are helpful for further clarifying the effective forms of ARTF. MDPI 2020-11-26 /pmc/articles/PMC7729672/ /pubmed/33256251 http://dx.doi.org/10.3390/molecules25235560 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Li-Jia
Li, Hong-Fu
Xu, Feng
Wang, Hong-Yan
Zhang, Yi-Fan
Liu, Guang-Xue
Shang, Ming-Ying
Wang, Xuan
Cai, Shao-Qing
Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS(n)
title Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS(n)
title_full Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS(n)
title_fullStr Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS(n)
title_full_unstemmed Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS(n)
title_short Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS(n)
title_sort exploring the in vivo existence forms (23 original constituents and 147 metabolites) of astragali radix total flavonoids and their distributions in rats using hplc-dad-esi-it-tof-ms(n)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729672/
https://www.ncbi.nlm.nih.gov/pubmed/33256251
http://dx.doi.org/10.3390/molecules25235560
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