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Cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness

Cell–cell fusion is a physiological process that is hijacked during oncogenesis and promotes tumour evolution. The main known impact of cell fusion is to promote the formation of metastatic hybrid cells following fusion between mobile leucocytes and proliferating tumour cells. We show here that cell...

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Autores principales: Delespaul, Lucile, Gélabert, Caroline, Lesluyes, Tom, Le Guellec, Sophie, Pérot, Gaëlle, Leroy, Laura, Baud, Jessica, Merle, Candice, Lartigue, Lydia, Chibon, Frédéric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729932/
https://www.ncbi.nlm.nih.gov/pubmed/33303824
http://dx.doi.org/10.1038/s41598-020-78502-z
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author Delespaul, Lucile
Gélabert, Caroline
Lesluyes, Tom
Le Guellec, Sophie
Pérot, Gaëlle
Leroy, Laura
Baud, Jessica
Merle, Candice
Lartigue, Lydia
Chibon, Frédéric
author_facet Delespaul, Lucile
Gélabert, Caroline
Lesluyes, Tom
Le Guellec, Sophie
Pérot, Gaëlle
Leroy, Laura
Baud, Jessica
Merle, Candice
Lartigue, Lydia
Chibon, Frédéric
author_sort Delespaul, Lucile
collection PubMed
description Cell–cell fusion is a physiological process that is hijacked during oncogenesis and promotes tumour evolution. The main known impact of cell fusion is to promote the formation of metastatic hybrid cells following fusion between mobile leucocytes and proliferating tumour cells. We show here that cell fusion between immortalized myoblasts and transformed fibroblasts, through genomic instability and expression of a specific transcriptomic profile, leads to emergence of hybrid cells acquiring dissemination properties. This is associated with acquisition of clonogenic ability by fused cells. In addition, by inheriting parental properties, hybrid tumours were found to mimic the histological characteristics of a specific histotype of sarcomas: undifferentiated pleomorphic sarcomas with incomplete muscular differentiation. This finding suggests that cell fusion, as macroevolution event, favours specific sarcoma development according to the differentiation lineage of parent cells.
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spelling pubmed-77299322020-12-14 Cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness Delespaul, Lucile Gélabert, Caroline Lesluyes, Tom Le Guellec, Sophie Pérot, Gaëlle Leroy, Laura Baud, Jessica Merle, Candice Lartigue, Lydia Chibon, Frédéric Sci Rep Article Cell–cell fusion is a physiological process that is hijacked during oncogenesis and promotes tumour evolution. The main known impact of cell fusion is to promote the formation of metastatic hybrid cells following fusion between mobile leucocytes and proliferating tumour cells. We show here that cell fusion between immortalized myoblasts and transformed fibroblasts, through genomic instability and expression of a specific transcriptomic profile, leads to emergence of hybrid cells acquiring dissemination properties. This is associated with acquisition of clonogenic ability by fused cells. In addition, by inheriting parental properties, hybrid tumours were found to mimic the histological characteristics of a specific histotype of sarcomas: undifferentiated pleomorphic sarcomas with incomplete muscular differentiation. This finding suggests that cell fusion, as macroevolution event, favours specific sarcoma development according to the differentiation lineage of parent cells. Nature Publishing Group UK 2020-12-10 /pmc/articles/PMC7729932/ /pubmed/33303824 http://dx.doi.org/10.1038/s41598-020-78502-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Delespaul, Lucile
Gélabert, Caroline
Lesluyes, Tom
Le Guellec, Sophie
Pérot, Gaëlle
Leroy, Laura
Baud, Jessica
Merle, Candice
Lartigue, Lydia
Chibon, Frédéric
Cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness
title Cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness
title_full Cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness
title_fullStr Cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness
title_full_unstemmed Cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness
title_short Cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness
title_sort cell–cell fusion of mesenchymal cells with distinct differentiations triggers genomic and transcriptomic remodelling toward tumour aggressiveness
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729932/
https://www.ncbi.nlm.nih.gov/pubmed/33303824
http://dx.doi.org/10.1038/s41598-020-78502-z
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