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Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study

(1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozo...

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Autores principales: Nardi, Gianna Maria, Fais, Sara, Casu, Cinzia, Mazur, Marta, Di Giorgio, Roberto, Grassi, Roberta, Grassi, Felice Roberto, Orrù, Germano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730335/
https://www.ncbi.nlm.nih.gov/pubmed/33291253
http://dx.doi.org/10.3390/ijerph17239106
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author Nardi, Gianna Maria
Fais, Sara
Casu, Cinzia
Mazur, Marta
Di Giorgio, Roberto
Grassi, Roberta
Grassi, Felice Roberto
Orrù, Germano
author_facet Nardi, Gianna Maria
Fais, Sara
Casu, Cinzia
Mazur, Marta
Di Giorgio, Roberto
Grassi, Roberta
Grassi, Felice Roberto
Orrù, Germano
author_sort Nardi, Gianna Maria
collection PubMed
description (1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozonated olive oil against Streptococcus mutans. (2) Methods: Two different commercial mouthwashes were tested: Ialozon Blu (IB) (Gemavip, Cagliari, Italy), with ozonated olive oil, and Ialozon Rose (IR) (Gemavip, Cagliari, Italy), with ozonated olive oil, hyaluronic acid and vitamin E. All formulates were analyzed in a dilution range from 2- to 256-folds in saline solution, as to reproduce the salivary dilution. Streptococcus mutans CIP103220 strain was used for the antimicrobial susceptibility test, and the Kirby–Bauer inhibition method was performed to evaluate the Minimum Inhibitory (MIC), Minimum Bactericidal (MBC), and Minimum Biofilm Inhibitory Concentration (MBIC). (3) Results: Both formulates showed the same antimicrobial activity. MIC, MBC, and MBIC were observed for dilution factors of 1/32, 1/8 and 1/8, respectively. The mean value of inhibition zone diameter was 16.5 mm for IB, and 18 mm for IR. (4) Conclusions: The results suggested that ozonized olive oil formulates were able to inactivate Streptococcus mutans avoiding the salivary dilution effect in the oral cavity.
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spelling pubmed-77303352020-12-12 Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study Nardi, Gianna Maria Fais, Sara Casu, Cinzia Mazur, Marta Di Giorgio, Roberto Grassi, Roberta Grassi, Felice Roberto Orrù, Germano Int J Environ Res Public Health Brief Report (1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozonated olive oil against Streptococcus mutans. (2) Methods: Two different commercial mouthwashes were tested: Ialozon Blu (IB) (Gemavip, Cagliari, Italy), with ozonated olive oil, and Ialozon Rose (IR) (Gemavip, Cagliari, Italy), with ozonated olive oil, hyaluronic acid and vitamin E. All formulates were analyzed in a dilution range from 2- to 256-folds in saline solution, as to reproduce the salivary dilution. Streptococcus mutans CIP103220 strain was used for the antimicrobial susceptibility test, and the Kirby–Bauer inhibition method was performed to evaluate the Minimum Inhibitory (MIC), Minimum Bactericidal (MBC), and Minimum Biofilm Inhibitory Concentration (MBIC). (3) Results: Both formulates showed the same antimicrobial activity. MIC, MBC, and MBIC were observed for dilution factors of 1/32, 1/8 and 1/8, respectively. The mean value of inhibition zone diameter was 16.5 mm for IB, and 18 mm for IR. (4) Conclusions: The results suggested that ozonized olive oil formulates were able to inactivate Streptococcus mutans avoiding the salivary dilution effect in the oral cavity. MDPI 2020-12-06 2020-12 /pmc/articles/PMC7730335/ /pubmed/33291253 http://dx.doi.org/10.3390/ijerph17239106 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Nardi, Gianna Maria
Fais, Sara
Casu, Cinzia
Mazur, Marta
Di Giorgio, Roberto
Grassi, Roberta
Grassi, Felice Roberto
Orrù, Germano
Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study
title Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study
title_full Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study
title_fullStr Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study
title_full_unstemmed Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study
title_short Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study
title_sort mouthwash based on ozonated olive oil in caries prevention: a preliminary in-vitro study
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730335/
https://www.ncbi.nlm.nih.gov/pubmed/33291253
http://dx.doi.org/10.3390/ijerph17239106
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