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Temperature and Inoculum Origin Influence the Performance of Ex-Situ Biological Hydrogen Methanation
The conversion of H(2) into methane can be carried out by microorganisms in a process so-called biomethanation. In ex-situ biomethanation H(2) and CO(2) gas are exogenous to the system. One of the main limitations of the biomethanation process is the low gas-liquid transfer rate and solubility of H(...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730501/ https://www.ncbi.nlm.nih.gov/pubmed/33271799 http://dx.doi.org/10.3390/molecules25235665 |
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author | Figeac, Noémie Trably, Eric Bernet, Nicolas Delgenès, Jean-Philippe Escudié, Renaud |
author_facet | Figeac, Noémie Trably, Eric Bernet, Nicolas Delgenès, Jean-Philippe Escudié, Renaud |
author_sort | Figeac, Noémie |
collection | PubMed |
description | The conversion of H(2) into methane can be carried out by microorganisms in a process so-called biomethanation. In ex-situ biomethanation H(2) and CO(2) gas are exogenous to the system. One of the main limitations of the biomethanation process is the low gas-liquid transfer rate and solubility of H(2) which are strongly influenced by the temperature. Hydrogenotrophic methanogens that are responsible for the biomethanation reaction are also very sensitive to temperature variations. The aim of this work was to evaluate the impact of temperature on batch biomethanation process in mixed culture. The performances of mesophilic and thermophilic inocula were assessed at 4 temperatures (24, 35, 55 and 65 °C). A negative impact of the low temperature (24 °C) was observed on microbial kinetics. Although methane production rate was higher at 55 and 65 °C (respectively 290 ± 55 and 309 ± 109 mL CH(4)/L.day for the mesophilic inoculum) than at 24 and 35 °C (respectively 156 ± 41 and 253 ± 51 mL CH(4)/L.day), the instability of the system substantially increased, likely because of a strong dominance of only Methanothermobacter species. Considering the maximal methane production rates and their stability all along the experiments, an optimal temperature range of 35 °C or 55 °C is recommended to operate ex-situ biomethanation process. |
format | Online Article Text |
id | pubmed-7730501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77305012020-12-12 Temperature and Inoculum Origin Influence the Performance of Ex-Situ Biological Hydrogen Methanation Figeac, Noémie Trably, Eric Bernet, Nicolas Delgenès, Jean-Philippe Escudié, Renaud Molecules Article The conversion of H(2) into methane can be carried out by microorganisms in a process so-called biomethanation. In ex-situ biomethanation H(2) and CO(2) gas are exogenous to the system. One of the main limitations of the biomethanation process is the low gas-liquid transfer rate and solubility of H(2) which are strongly influenced by the temperature. Hydrogenotrophic methanogens that are responsible for the biomethanation reaction are also very sensitive to temperature variations. The aim of this work was to evaluate the impact of temperature on batch biomethanation process in mixed culture. The performances of mesophilic and thermophilic inocula were assessed at 4 temperatures (24, 35, 55 and 65 °C). A negative impact of the low temperature (24 °C) was observed on microbial kinetics. Although methane production rate was higher at 55 and 65 °C (respectively 290 ± 55 and 309 ± 109 mL CH(4)/L.day for the mesophilic inoculum) than at 24 and 35 °C (respectively 156 ± 41 and 253 ± 51 mL CH(4)/L.day), the instability of the system substantially increased, likely because of a strong dominance of only Methanothermobacter species. Considering the maximal methane production rates and their stability all along the experiments, an optimal temperature range of 35 °C or 55 °C is recommended to operate ex-situ biomethanation process. MDPI 2020-12-01 /pmc/articles/PMC7730501/ /pubmed/33271799 http://dx.doi.org/10.3390/molecules25235665 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Figeac, Noémie Trably, Eric Bernet, Nicolas Delgenès, Jean-Philippe Escudié, Renaud Temperature and Inoculum Origin Influence the Performance of Ex-Situ Biological Hydrogen Methanation |
title | Temperature and Inoculum Origin Influence the Performance of Ex-Situ Biological Hydrogen Methanation |
title_full | Temperature and Inoculum Origin Influence the Performance of Ex-Situ Biological Hydrogen Methanation |
title_fullStr | Temperature and Inoculum Origin Influence the Performance of Ex-Situ Biological Hydrogen Methanation |
title_full_unstemmed | Temperature and Inoculum Origin Influence the Performance of Ex-Situ Biological Hydrogen Methanation |
title_short | Temperature and Inoculum Origin Influence the Performance of Ex-Situ Biological Hydrogen Methanation |
title_sort | temperature and inoculum origin influence the performance of ex-situ biological hydrogen methanation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730501/ https://www.ncbi.nlm.nih.gov/pubmed/33271799 http://dx.doi.org/10.3390/molecules25235665 |
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