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Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles

Our groups have previously developed a biochemical gas sensor to measure isopropanol (IPA) in exhaled air and have applied it for breath IPA investigation in healthy subjects and diabetes patients. In this study, the original bio-sniffer was modified with a series of components that improved the lim...

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Autores principales: Chien, Po-Jen, Suzuki, Takuma, Ye, Ming, Toma, Koji, Arakawa, Takahiro, Iwasaki, Yasuhiko, Mitsubayashi, Kohji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730524/
https://www.ncbi.nlm.nih.gov/pubmed/33260380
http://dx.doi.org/10.3390/s20236827
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author Chien, Po-Jen
Suzuki, Takuma
Ye, Ming
Toma, Koji
Arakawa, Takahiro
Iwasaki, Yasuhiko
Mitsubayashi, Kohji
author_facet Chien, Po-Jen
Suzuki, Takuma
Ye, Ming
Toma, Koji
Arakawa, Takahiro
Iwasaki, Yasuhiko
Mitsubayashi, Kohji
author_sort Chien, Po-Jen
collection PubMed
description Our groups have previously developed a biochemical gas sensor to measure isopropanol (IPA) in exhaled air and have applied it for breath IPA investigation in healthy subjects and diabetes patients. In this study, the original bio-sniffer was modified with a series of components that improved the limit of detection (LOD). First, the modified IPA bio-sniffer used a C8855-type photomultiplier tube (PMT) that performed well in the photon sensitivity at the peak wavelength of nicotinamide adenine dinucleotide (NADH) fluorescence. Second, the multi-core bifurcated optical fiber, which incorporated 36 fibers to replace the previous dual-core type, enhanced the fluorescence collection. Third, the optical fiber probe was reinforced for greater width, and the flow-cell was redesigned to increase the area of the enzyme-immobilized membrane in contact with the air sample. These modifications lowered the detection limit to 0.5 ppb, a significant increase over the previous 1.0 ppb. Moreover, the modified bio-sniffer successfully analyzed the IPA concentration in exhaled air from a volunteer, which confirmed its capability for real-world sample detection. The modified bio-sniffer is more applicable to breath measurement and the detection of other extremely-low-concentration samples.
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spelling pubmed-77305242020-12-12 Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles Chien, Po-Jen Suzuki, Takuma Ye, Ming Toma, Koji Arakawa, Takahiro Iwasaki, Yasuhiko Mitsubayashi, Kohji Sensors (Basel) Article Our groups have previously developed a biochemical gas sensor to measure isopropanol (IPA) in exhaled air and have applied it for breath IPA investigation in healthy subjects and diabetes patients. In this study, the original bio-sniffer was modified with a series of components that improved the limit of detection (LOD). First, the modified IPA bio-sniffer used a C8855-type photomultiplier tube (PMT) that performed well in the photon sensitivity at the peak wavelength of nicotinamide adenine dinucleotide (NADH) fluorescence. Second, the multi-core bifurcated optical fiber, which incorporated 36 fibers to replace the previous dual-core type, enhanced the fluorescence collection. Third, the optical fiber probe was reinforced for greater width, and the flow-cell was redesigned to increase the area of the enzyme-immobilized membrane in contact with the air sample. These modifications lowered the detection limit to 0.5 ppb, a significant increase over the previous 1.0 ppb. Moreover, the modified bio-sniffer successfully analyzed the IPA concentration in exhaled air from a volunteer, which confirmed its capability for real-world sample detection. The modified bio-sniffer is more applicable to breath measurement and the detection of other extremely-low-concentration samples. MDPI 2020-11-29 /pmc/articles/PMC7730524/ /pubmed/33260380 http://dx.doi.org/10.3390/s20236827 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chien, Po-Jen
Suzuki, Takuma
Ye, Ming
Toma, Koji
Arakawa, Takahiro
Iwasaki, Yasuhiko
Mitsubayashi, Kohji
Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles
title Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles
title_full Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles
title_fullStr Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles
title_full_unstemmed Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles
title_short Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles
title_sort ultra-sensitive isopropanol biochemical gas sensor (bio-sniffer) for monitoring of human volatiles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730524/
https://www.ncbi.nlm.nih.gov/pubmed/33260380
http://dx.doi.org/10.3390/s20236827
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