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Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells

With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth’s magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been well ch...

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Autores principales: Xue, Xunwen, Ali, Yasser F., Liu, Caorui, Hong, Zhiqiang, Luo, Wanrong, Nie, Jing, Li, Bingyan, Jiao, Yang, Liu, Ning-Ang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730591/
https://www.ncbi.nlm.nih.gov/pubmed/33291307
http://dx.doi.org/10.3390/ijms21239304
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author Xue, Xunwen
Ali, Yasser F.
Liu, Caorui
Hong, Zhiqiang
Luo, Wanrong
Nie, Jing
Li, Bingyan
Jiao, Yang
Liu, Ning-Ang
author_facet Xue, Xunwen
Ali, Yasser F.
Liu, Caorui
Hong, Zhiqiang
Luo, Wanrong
Nie, Jing
Li, Bingyan
Jiao, Yang
Liu, Ning-Ang
author_sort Xue, Xunwen
collection PubMed
description With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth’s magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been well characterized so far. In this study, immortalized human bronchial epithelial cells were exposed to X-rays under either a geomagnetic field (GMF, ~50 uT) or HMF (<50 nT) culture condition. A significant increase of the cell survival rate in HMF after radiation was observed by colony formation analysis. The kinetics of DNA double-strand breaks (DSBs), determined by γH2AX foci formation and disappearance, presented a faster decrease of foci-positive cells and a significantly lower mean number of γH2AX foci per nucleus in HMF-cultured cells than in GMF-cultured cells after radiation. In addition, a γH2AX/53BP1 colocalization assay showed an upregulated DSB recovery rate in HMF cultured cells. These findings provided the first evidence that HMF exposure may enhance the cellular DSB repair efficiency upon radiation, and consequently modulate the genotoxic effects of IR.
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spelling pubmed-77305912020-12-12 Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells Xue, Xunwen Ali, Yasser F. Liu, Caorui Hong, Zhiqiang Luo, Wanrong Nie, Jing Li, Bingyan Jiao, Yang Liu, Ning-Ang Int J Mol Sci Brief Report With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth’s magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been well characterized so far. In this study, immortalized human bronchial epithelial cells were exposed to X-rays under either a geomagnetic field (GMF, ~50 uT) or HMF (<50 nT) culture condition. A significant increase of the cell survival rate in HMF after radiation was observed by colony formation analysis. The kinetics of DNA double-strand breaks (DSBs), determined by γH2AX foci formation and disappearance, presented a faster decrease of foci-positive cells and a significantly lower mean number of γH2AX foci per nucleus in HMF-cultured cells than in GMF-cultured cells after radiation. In addition, a γH2AX/53BP1 colocalization assay showed an upregulated DSB recovery rate in HMF cultured cells. These findings provided the first evidence that HMF exposure may enhance the cellular DSB repair efficiency upon radiation, and consequently modulate the genotoxic effects of IR. MDPI 2020-12-06 /pmc/articles/PMC7730591/ /pubmed/33291307 http://dx.doi.org/10.3390/ijms21239304 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Xue, Xunwen
Ali, Yasser F.
Liu, Caorui
Hong, Zhiqiang
Luo, Wanrong
Nie, Jing
Li, Bingyan
Jiao, Yang
Liu, Ning-Ang
Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells
title Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells
title_full Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells
title_fullStr Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells
title_full_unstemmed Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells
title_short Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells
title_sort geomagnetic shielding enhances radiation resistance by promoting dna repair process in human bronchial epithelial cells
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730591/
https://www.ncbi.nlm.nih.gov/pubmed/33291307
http://dx.doi.org/10.3390/ijms21239304
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