Cargando…
Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells
With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth’s magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been well ch...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730591/ https://www.ncbi.nlm.nih.gov/pubmed/33291307 http://dx.doi.org/10.3390/ijms21239304 |
_version_ | 1783621718747643904 |
---|---|
author | Xue, Xunwen Ali, Yasser F. Liu, Caorui Hong, Zhiqiang Luo, Wanrong Nie, Jing Li, Bingyan Jiao, Yang Liu, Ning-Ang |
author_facet | Xue, Xunwen Ali, Yasser F. Liu, Caorui Hong, Zhiqiang Luo, Wanrong Nie, Jing Li, Bingyan Jiao, Yang Liu, Ning-Ang |
author_sort | Xue, Xunwen |
collection | PubMed |
description | With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth’s magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been well characterized so far. In this study, immortalized human bronchial epithelial cells were exposed to X-rays under either a geomagnetic field (GMF, ~50 uT) or HMF (<50 nT) culture condition. A significant increase of the cell survival rate in HMF after radiation was observed by colony formation analysis. The kinetics of DNA double-strand breaks (DSBs), determined by γH2AX foci formation and disappearance, presented a faster decrease of foci-positive cells and a significantly lower mean number of γH2AX foci per nucleus in HMF-cultured cells than in GMF-cultured cells after radiation. In addition, a γH2AX/53BP1 colocalization assay showed an upregulated DSB recovery rate in HMF cultured cells. These findings provided the first evidence that HMF exposure may enhance the cellular DSB repair efficiency upon radiation, and consequently modulate the genotoxic effects of IR. |
format | Online Article Text |
id | pubmed-7730591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77305912020-12-12 Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells Xue, Xunwen Ali, Yasser F. Liu, Caorui Hong, Zhiqiang Luo, Wanrong Nie, Jing Li, Bingyan Jiao, Yang Liu, Ning-Ang Int J Mol Sci Brief Report With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth’s magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been well characterized so far. In this study, immortalized human bronchial epithelial cells were exposed to X-rays under either a geomagnetic field (GMF, ~50 uT) or HMF (<50 nT) culture condition. A significant increase of the cell survival rate in HMF after radiation was observed by colony formation analysis. The kinetics of DNA double-strand breaks (DSBs), determined by γH2AX foci formation and disappearance, presented a faster decrease of foci-positive cells and a significantly lower mean number of γH2AX foci per nucleus in HMF-cultured cells than in GMF-cultured cells after radiation. In addition, a γH2AX/53BP1 colocalization assay showed an upregulated DSB recovery rate in HMF cultured cells. These findings provided the first evidence that HMF exposure may enhance the cellular DSB repair efficiency upon radiation, and consequently modulate the genotoxic effects of IR. MDPI 2020-12-06 /pmc/articles/PMC7730591/ /pubmed/33291307 http://dx.doi.org/10.3390/ijms21239304 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Xue, Xunwen Ali, Yasser F. Liu, Caorui Hong, Zhiqiang Luo, Wanrong Nie, Jing Li, Bingyan Jiao, Yang Liu, Ning-Ang Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells |
title | Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells |
title_full | Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells |
title_fullStr | Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells |
title_full_unstemmed | Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells |
title_short | Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells |
title_sort | geomagnetic shielding enhances radiation resistance by promoting dna repair process in human bronchial epithelial cells |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730591/ https://www.ncbi.nlm.nih.gov/pubmed/33291307 http://dx.doi.org/10.3390/ijms21239304 |
work_keys_str_mv | AT xuexunwen geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells AT aliyasserf geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells AT liucaorui geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells AT hongzhiqiang geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells AT luowanrong geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells AT niejing geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells AT libingyan geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells AT jiaoyang geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells AT liuningang geomagneticshieldingenhancesradiationresistancebypromotingdnarepairprocessinhumanbronchialepithelialcells |