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Comparative Analysis of ROS Network Genes in Extremophile Eukaryotes

The reactive oxygen species (ROS) gene network, consisting of both ROS-generating and detoxifying enzymes, adjusts ROS levels in response to various stimuli. We performed a cross-kingdom comparison of ROS gene networks to investigate how they have evolved across all Eukaryotes, including protists, f...

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Autores principales: Lyall, Rafe, Nikoloski, Zoran, Gechev, Tsanko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730656/
https://www.ncbi.nlm.nih.gov/pubmed/33266251
http://dx.doi.org/10.3390/ijms21239131
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author Lyall, Rafe
Nikoloski, Zoran
Gechev, Tsanko
author_facet Lyall, Rafe
Nikoloski, Zoran
Gechev, Tsanko
author_sort Lyall, Rafe
collection PubMed
description The reactive oxygen species (ROS) gene network, consisting of both ROS-generating and detoxifying enzymes, adjusts ROS levels in response to various stimuli. We performed a cross-kingdom comparison of ROS gene networks to investigate how they have evolved across all Eukaryotes, including protists, fungi, plants and animals. We included the genomes of 16 extremotolerant Eukaryotes to gain insight into ROS gene evolution in organisms that experience extreme stress conditions. Our analysis focused on ROS genes found in all Eukaryotes (such as catalases, superoxide dismutases, glutathione reductases, peroxidases and glutathione peroxidase/peroxiredoxins) as well as those specific to certain groups, such as ascorbate peroxidases, dehydroascorbate/monodehydroascorbate reductases in plants and other photosynthetic organisms. ROS-producing NADPH oxidases (NOX) were found in most multicellular organisms, although several NOX-like genes were identified in unicellular or filamentous species. However, despite the extreme conditions experienced by extremophile species, we found no evidence for expansion of ROS-related gene families in these species compared to other Eukaryotes. Tardigrades and rotifers do show ROS gene expansions that could be related to their extreme lifestyles, although a high rate of lineage-specific horizontal gene transfer events, coupled with recent tetraploidy in rotifers, could explain this observation. This suggests that the basal Eukaryotic ROS scavenging systems are sufficient to maintain ROS homeostasis even under the most extreme conditions.
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spelling pubmed-77306562020-12-12 Comparative Analysis of ROS Network Genes in Extremophile Eukaryotes Lyall, Rafe Nikoloski, Zoran Gechev, Tsanko Int J Mol Sci Article The reactive oxygen species (ROS) gene network, consisting of both ROS-generating and detoxifying enzymes, adjusts ROS levels in response to various stimuli. We performed a cross-kingdom comparison of ROS gene networks to investigate how they have evolved across all Eukaryotes, including protists, fungi, plants and animals. We included the genomes of 16 extremotolerant Eukaryotes to gain insight into ROS gene evolution in organisms that experience extreme stress conditions. Our analysis focused on ROS genes found in all Eukaryotes (such as catalases, superoxide dismutases, glutathione reductases, peroxidases and glutathione peroxidase/peroxiredoxins) as well as those specific to certain groups, such as ascorbate peroxidases, dehydroascorbate/monodehydroascorbate reductases in plants and other photosynthetic organisms. ROS-producing NADPH oxidases (NOX) were found in most multicellular organisms, although several NOX-like genes were identified in unicellular or filamentous species. However, despite the extreme conditions experienced by extremophile species, we found no evidence for expansion of ROS-related gene families in these species compared to other Eukaryotes. Tardigrades and rotifers do show ROS gene expansions that could be related to their extreme lifestyles, although a high rate of lineage-specific horizontal gene transfer events, coupled with recent tetraploidy in rotifers, could explain this observation. This suggests that the basal Eukaryotic ROS scavenging systems are sufficient to maintain ROS homeostasis even under the most extreme conditions. MDPI 2020-11-30 /pmc/articles/PMC7730656/ /pubmed/33266251 http://dx.doi.org/10.3390/ijms21239131 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lyall, Rafe
Nikoloski, Zoran
Gechev, Tsanko
Comparative Analysis of ROS Network Genes in Extremophile Eukaryotes
title Comparative Analysis of ROS Network Genes in Extremophile Eukaryotes
title_full Comparative Analysis of ROS Network Genes in Extremophile Eukaryotes
title_fullStr Comparative Analysis of ROS Network Genes in Extremophile Eukaryotes
title_full_unstemmed Comparative Analysis of ROS Network Genes in Extremophile Eukaryotes
title_short Comparative Analysis of ROS Network Genes in Extremophile Eukaryotes
title_sort comparative analysis of ros network genes in extremophile eukaryotes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730656/
https://www.ncbi.nlm.nih.gov/pubmed/33266251
http://dx.doi.org/10.3390/ijms21239131
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