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Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues

This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individu...

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Autores principales: Figueiredo, Luciene Cristina, Bueno-Silva, Bruno, Nogueira, Cristiana Fernandes Plutarco, Valadares, Leonardo Carneiro, Garcia, Katia Marina Morilla, Filho, Givelton Coimbra da Luz, Milanello, Luciano, Esteves, Felipe Machado, Shibli, Jamil Awad, Miranda, Tamires Szeremeske
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730812/
https://www.ncbi.nlm.nih.gov/pubmed/33291232
http://dx.doi.org/10.3390/ijerph17239100
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author Figueiredo, Luciene Cristina
Bueno-Silva, Bruno
Nogueira, Cristiana Fernandes Plutarco
Valadares, Leonardo Carneiro
Garcia, Katia Marina Morilla
Filho, Givelton Coimbra da Luz
Milanello, Luciano
Esteves, Felipe Machado
Shibli, Jamil Awad
Miranda, Tamires Szeremeske
author_facet Figueiredo, Luciene Cristina
Bueno-Silva, Bruno
Nogueira, Cristiana Fernandes Plutarco
Valadares, Leonardo Carneiro
Garcia, Katia Marina Morilla
Filho, Givelton Coimbra da Luz
Milanello, Luciano
Esteves, Felipe Machado
Shibli, Jamil Awad
Miranda, Tamires Szeremeske
author_sort Figueiredo, Luciene Cristina
collection PubMed
description This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individuals with periodontal and peri-implant disease. The study population included individuals with four periodontal statuses: periodontal health (PH group, n = 20); periodontitis (P group, n = 20); peri-implant health (PIH group, n = 20), and peri-implantitis (PI group, n = 20). Gingival biopsies were collected from one tooth per patient according to the inclusion criteria of each group. The mRNA levels of IL-6, IL-1ß, TNF-α, MMP-1, MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2 were evaluated by qPCR. The levels of IL-1ß were significantly higher in the PI group when compared to the other groups (p < 0.05), while the levels of IL-6 were significantly higher in the groups with periodontal and peri-implant disease when compared with the healthy groups (p < 0.05); however, the levels of IL-6 did not differ between the PI and P groups (p > 0.05). For all other studied biomarkers, no significant differences were observed between groups (p > 0.05). IL-6 and IL-1ß presented higher levels of mRNA in diseased periodontal and peri-implant tissues. However, the expression of metalloproteinases and their inhibitors did not differ between the different periodontal statuses.
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spelling pubmed-77308122020-12-12 Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues Figueiredo, Luciene Cristina Bueno-Silva, Bruno Nogueira, Cristiana Fernandes Plutarco Valadares, Leonardo Carneiro Garcia, Katia Marina Morilla Filho, Givelton Coimbra da Luz Milanello, Luciano Esteves, Felipe Machado Shibli, Jamil Awad Miranda, Tamires Szeremeske Int J Environ Res Public Health Article This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individuals with periodontal and peri-implant disease. The study population included individuals with four periodontal statuses: periodontal health (PH group, n = 20); periodontitis (P group, n = 20); peri-implant health (PIH group, n = 20), and peri-implantitis (PI group, n = 20). Gingival biopsies were collected from one tooth per patient according to the inclusion criteria of each group. The mRNA levels of IL-6, IL-1ß, TNF-α, MMP-1, MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2 were evaluated by qPCR. The levels of IL-1ß were significantly higher in the PI group when compared to the other groups (p < 0.05), while the levels of IL-6 were significantly higher in the groups with periodontal and peri-implant disease when compared with the healthy groups (p < 0.05); however, the levels of IL-6 did not differ between the PI and P groups (p > 0.05). For all other studied biomarkers, no significant differences were observed between groups (p > 0.05). IL-6 and IL-1ß presented higher levels of mRNA in diseased periodontal and peri-implant tissues. However, the expression of metalloproteinases and their inhibitors did not differ between the different periodontal statuses. MDPI 2020-12-06 2020-12 /pmc/articles/PMC7730812/ /pubmed/33291232 http://dx.doi.org/10.3390/ijerph17239100 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Figueiredo, Luciene Cristina
Bueno-Silva, Bruno
Nogueira, Cristiana Fernandes Plutarco
Valadares, Leonardo Carneiro
Garcia, Katia Marina Morilla
Filho, Givelton Coimbra da Luz
Milanello, Luciano
Esteves, Felipe Machado
Shibli, Jamil Awad
Miranda, Tamires Szeremeske
Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues
title Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues
title_full Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues
title_fullStr Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues
title_full_unstemmed Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues
title_short Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues
title_sort levels of gene expression of immunological biomarkers in peri-implant and periodontal tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730812/
https://www.ncbi.nlm.nih.gov/pubmed/33291232
http://dx.doi.org/10.3390/ijerph17239100
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