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Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues
This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730812/ https://www.ncbi.nlm.nih.gov/pubmed/33291232 http://dx.doi.org/10.3390/ijerph17239100 |
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author | Figueiredo, Luciene Cristina Bueno-Silva, Bruno Nogueira, Cristiana Fernandes Plutarco Valadares, Leonardo Carneiro Garcia, Katia Marina Morilla Filho, Givelton Coimbra da Luz Milanello, Luciano Esteves, Felipe Machado Shibli, Jamil Awad Miranda, Tamires Szeremeske |
author_facet | Figueiredo, Luciene Cristina Bueno-Silva, Bruno Nogueira, Cristiana Fernandes Plutarco Valadares, Leonardo Carneiro Garcia, Katia Marina Morilla Filho, Givelton Coimbra da Luz Milanello, Luciano Esteves, Felipe Machado Shibli, Jamil Awad Miranda, Tamires Szeremeske |
author_sort | Figueiredo, Luciene Cristina |
collection | PubMed |
description | This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individuals with periodontal and peri-implant disease. The study population included individuals with four periodontal statuses: periodontal health (PH group, n = 20); periodontitis (P group, n = 20); peri-implant health (PIH group, n = 20), and peri-implantitis (PI group, n = 20). Gingival biopsies were collected from one tooth per patient according to the inclusion criteria of each group. The mRNA levels of IL-6, IL-1ß, TNF-α, MMP-1, MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2 were evaluated by qPCR. The levels of IL-1ß were significantly higher in the PI group when compared to the other groups (p < 0.05), while the levels of IL-6 were significantly higher in the groups with periodontal and peri-implant disease when compared with the healthy groups (p < 0.05); however, the levels of IL-6 did not differ between the PI and P groups (p > 0.05). For all other studied biomarkers, no significant differences were observed between groups (p > 0.05). IL-6 and IL-1ß presented higher levels of mRNA in diseased periodontal and peri-implant tissues. However, the expression of metalloproteinases and their inhibitors did not differ between the different periodontal statuses. |
format | Online Article Text |
id | pubmed-7730812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77308122020-12-12 Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues Figueiredo, Luciene Cristina Bueno-Silva, Bruno Nogueira, Cristiana Fernandes Plutarco Valadares, Leonardo Carneiro Garcia, Katia Marina Morilla Filho, Givelton Coimbra da Luz Milanello, Luciano Esteves, Felipe Machado Shibli, Jamil Awad Miranda, Tamires Szeremeske Int J Environ Res Public Health Article This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individuals with periodontal and peri-implant disease. The study population included individuals with four periodontal statuses: periodontal health (PH group, n = 20); periodontitis (P group, n = 20); peri-implant health (PIH group, n = 20), and peri-implantitis (PI group, n = 20). Gingival biopsies were collected from one tooth per patient according to the inclusion criteria of each group. The mRNA levels of IL-6, IL-1ß, TNF-α, MMP-1, MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2 were evaluated by qPCR. The levels of IL-1ß were significantly higher in the PI group when compared to the other groups (p < 0.05), while the levels of IL-6 were significantly higher in the groups with periodontal and peri-implant disease when compared with the healthy groups (p < 0.05); however, the levels of IL-6 did not differ between the PI and P groups (p > 0.05). For all other studied biomarkers, no significant differences were observed between groups (p > 0.05). IL-6 and IL-1ß presented higher levels of mRNA in diseased periodontal and peri-implant tissues. However, the expression of metalloproteinases and their inhibitors did not differ between the different periodontal statuses. MDPI 2020-12-06 2020-12 /pmc/articles/PMC7730812/ /pubmed/33291232 http://dx.doi.org/10.3390/ijerph17239100 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Figueiredo, Luciene Cristina Bueno-Silva, Bruno Nogueira, Cristiana Fernandes Plutarco Valadares, Leonardo Carneiro Garcia, Katia Marina Morilla Filho, Givelton Coimbra da Luz Milanello, Luciano Esteves, Felipe Machado Shibli, Jamil Awad Miranda, Tamires Szeremeske Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues |
title | Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues |
title_full | Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues |
title_fullStr | Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues |
title_full_unstemmed | Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues |
title_short | Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues |
title_sort | levels of gene expression of immunological biomarkers in peri-implant and periodontal tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730812/ https://www.ncbi.nlm.nih.gov/pubmed/33291232 http://dx.doi.org/10.3390/ijerph17239100 |
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