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Comparison of Bisulfite Pyrosequencing and Methylation-Specific qPCR for Methylation Assessment

Different methodological approaches are available to assess DNA methylation biomarkers. In this study, we evaluated two sodium bisulfite conversion-dependent methods, namely pyrosequencing and methylation-specific qPCR (MS-qPCR), with the aim of measuring the closeness of agreement of methylation va...

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Detalles Bibliográficos
Autores principales: De Chiara, Loretta, Leiro-Fernandez, Virginia, Rodríguez-Girondo, Mar, Valverde, Diana, Botana-Rial, María Isabel, Fernández-Villar, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730915/
https://www.ncbi.nlm.nih.gov/pubmed/33287451
http://dx.doi.org/10.3390/ijms21239242