Cargando…
Comparison of Bisulfite Pyrosequencing and Methylation-Specific qPCR for Methylation Assessment
Different methodological approaches are available to assess DNA methylation biomarkers. In this study, we evaluated two sodium bisulfite conversion-dependent methods, namely pyrosequencing and methylation-specific qPCR (MS-qPCR), with the aim of measuring the closeness of agreement of methylation va...
Autores principales: | De Chiara, Loretta, Leiro-Fernandez, Virginia, Rodríguez-Girondo, Mar, Valverde, Diana, Botana-Rial, María Isabel, Fernández-Villar, Alberto |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730915/ https://www.ncbi.nlm.nih.gov/pubmed/33287451 http://dx.doi.org/10.3390/ijms21239242 |
Ejemplares similares
-
Methylation Assessment for the Prediction of Malignancy in Mediastinal Adenopathies Obtained by Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration in Patients with Lung Cancer
por: Leiro-Fernandez, Virginia, et al.
Publicado: (2019) -
Detection of pathogens in water: from phylochips to qPCR to pyrosequencing
por: Aw, Tiong Gim, et al.
Publicado: (2012) -
The use of Multiple Displacement Amplified DNA as a control for Methylation Specific PCR, Pyrosequencing, Bisulfite Sequencing and Methylation-Sensitive Restriction Enzyme PCR
por: Hughes, Simon, et al.
Publicado: (2007) -
Recovery of bisulfite-converted genomic sequences in the methylation-sensitive QPCR
por: Munson, Kristofer, et al.
Publicado: (2007) -
Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)