Cargando…

Withanolide-Type Steroids from Withania aristata as Potential Anti-Leukemic Agents

Leukemia is a blood or bone marrow cancer with increasing incidence in developed regions of the world. Currently, there is an ongoing need for novel and safe anti-leukemic agents, as no fully effective chemotherapy is available to treat this life-threatening disease. Herein, are reported the isolati...

Descripción completa

Detalles Bibliográficos
Autores principales: Moujir, Laila M., Llanos, Gabriel G., Araujo, Liliana, Amesty, Angel, Bazzocchi, Isabel L., Jiménez, Ignacio A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731379/
https://www.ncbi.nlm.nih.gov/pubmed/33291428
http://dx.doi.org/10.3390/molecules25235744
_version_ 1783621886169579520
author Moujir, Laila M.
Llanos, Gabriel G.
Araujo, Liliana
Amesty, Angel
Bazzocchi, Isabel L.
Jiménez, Ignacio A.
author_facet Moujir, Laila M.
Llanos, Gabriel G.
Araujo, Liliana
Amesty, Angel
Bazzocchi, Isabel L.
Jiménez, Ignacio A.
author_sort Moujir, Laila M.
collection PubMed
description Leukemia is a blood or bone marrow cancer with increasing incidence in developed regions of the world. Currently, there is an ongoing need for novel and safe anti-leukemic agents, as no fully effective chemotherapy is available to treat this life-threatening disease. Herein, are reported the isolation, structural elucidation, and anti-leukemic evaluation of twenty-nine withanolide-type steroids (1–29) from Withania aristata. Among them, the new isolated withanolides, withaperoxidins A–D (1–4) have an unusual six-membered cyclic peroxide moiety on the withasteroid skeleton as a structural novelty. Their structures have been elucidated by means of spectroscopic analyses, including 2D NMR experiments. In addition, extensive structure–activity relationships and in silico ADME studies were employed to understand the pharmacophore and pharmacokinetic properties of this series of withasteroids. Compounds 15, 16, and 22 together with withaferin A (14) were identified as having improved antiproliferative effect (IC(50) ranging from 0.2 to 0.7 μM) on human leukemia HL-60 cell lines compared with the reference drug, etoposide. This cytotoxic potency was also coupled with good selectivity index (SI 33.0–9.2) on non-tumoral Vero cell line and in silico drug likeness. These findings revealed that these natural withasteroids are potential candidates as chemotherapeutic agents in the treatment of leukemia.
format Online
Article
Text
id pubmed-7731379
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77313792020-12-12 Withanolide-Type Steroids from Withania aristata as Potential Anti-Leukemic Agents Moujir, Laila M. Llanos, Gabriel G. Araujo, Liliana Amesty, Angel Bazzocchi, Isabel L. Jiménez, Ignacio A. Molecules Article Leukemia is a blood or bone marrow cancer with increasing incidence in developed regions of the world. Currently, there is an ongoing need for novel and safe anti-leukemic agents, as no fully effective chemotherapy is available to treat this life-threatening disease. Herein, are reported the isolation, structural elucidation, and anti-leukemic evaluation of twenty-nine withanolide-type steroids (1–29) from Withania aristata. Among them, the new isolated withanolides, withaperoxidins A–D (1–4) have an unusual six-membered cyclic peroxide moiety on the withasteroid skeleton as a structural novelty. Their structures have been elucidated by means of spectroscopic analyses, including 2D NMR experiments. In addition, extensive structure–activity relationships and in silico ADME studies were employed to understand the pharmacophore and pharmacokinetic properties of this series of withasteroids. Compounds 15, 16, and 22 together with withaferin A (14) were identified as having improved antiproliferative effect (IC(50) ranging from 0.2 to 0.7 μM) on human leukemia HL-60 cell lines compared with the reference drug, etoposide. This cytotoxic potency was also coupled with good selectivity index (SI 33.0–9.2) on non-tumoral Vero cell line and in silico drug likeness. These findings revealed that these natural withasteroids are potential candidates as chemotherapeutic agents in the treatment of leukemia. MDPI 2020-12-05 /pmc/articles/PMC7731379/ /pubmed/33291428 http://dx.doi.org/10.3390/molecules25235744 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moujir, Laila M.
Llanos, Gabriel G.
Araujo, Liliana
Amesty, Angel
Bazzocchi, Isabel L.
Jiménez, Ignacio A.
Withanolide-Type Steroids from Withania aristata as Potential Anti-Leukemic Agents
title Withanolide-Type Steroids from Withania aristata as Potential Anti-Leukemic Agents
title_full Withanolide-Type Steroids from Withania aristata as Potential Anti-Leukemic Agents
title_fullStr Withanolide-Type Steroids from Withania aristata as Potential Anti-Leukemic Agents
title_full_unstemmed Withanolide-Type Steroids from Withania aristata as Potential Anti-Leukemic Agents
title_short Withanolide-Type Steroids from Withania aristata as Potential Anti-Leukemic Agents
title_sort withanolide-type steroids from withania aristata as potential anti-leukemic agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731379/
https://www.ncbi.nlm.nih.gov/pubmed/33291428
http://dx.doi.org/10.3390/molecules25235744
work_keys_str_mv AT moujirlailam withanolidetypesteroidsfromwithaniaaristataaspotentialantileukemicagents
AT llanosgabrielg withanolidetypesteroidsfromwithaniaaristataaspotentialantileukemicagents
AT araujoliliana withanolidetypesteroidsfromwithaniaaristataaspotentialantileukemicagents
AT amestyangel withanolidetypesteroidsfromwithaniaaristataaspotentialantileukemicagents
AT bazzocchiisabell withanolidetypesteroidsfromwithaniaaristataaspotentialantileukemicagents
AT jimenezignacioa withanolidetypesteroidsfromwithaniaaristataaspotentialantileukemicagents