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Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats

Our previous study documented that melatonin (MLT) induced the osteogenic differentiation of mesenchymal stem cells (MSCs) and promoted the healing of femoral fractures in rats via the neuropeptide Y (NPY)/neuropeptide Y1 receptor (NPY1R) signaling pathway. MLT treatment upregulated the expression o...

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Autores principales: Shang, Junjun, Ma, Bin, Zhu, Guiling, Dong, Penghong, Wang, Chan, Gu, Xiaochuan, Zi, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731576/
https://www.ncbi.nlm.nih.gov/pubmed/33334091
http://dx.doi.org/10.4081/ejh.2020.3183
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author Shang, Junjun
Ma, Bin
Zhu, Guiling
Dong, Penghong
Wang, Chan
Gu, Xiaochuan
Zi, Ying
author_facet Shang, Junjun
Ma, Bin
Zhu, Guiling
Dong, Penghong
Wang, Chan
Gu, Xiaochuan
Zi, Ying
author_sort Shang, Junjun
collection PubMed
description Our previous study documented that melatonin (MLT) induced the osteogenic differentiation of mesenchymal stem cells (MSCs) and promoted the healing of femoral fractures in rats via the neuropeptide Y (NPY)/neuropeptide Y1 receptor (NPY1R) signaling pathway. MLT treatment upregulated the expression of the zinc uptake transporter zinc transporter 1 (Zip1) in nerve cells. Prior research demonstrated that oral zinc upregulated NPY expression. MSCs were isolated from rat bone marrow and identified using flow cytometry in our study. The results showed that MLT treatment upregulated NPY and NPY1R levels in MSCs with osteogenic differentiation, which was accompanied by upregulated Zip1 expression. However, the MLT-induced osteogenic differentiation of MSCs was reversed after interference of Zip1 expression. It was confirmed by the decreased alkaline phosphatase (ALP) level; downregulated activities of type I collagen α1 chain (COL1A1), osteocalcin (OCN), runt-related transcription factor 2 (Runx2) and ALP; and reduced mineralized nodule formation. MLT promoted fracture healing in rats with femoral fracture, which was accompanied by increased expression of NPY and NPY1R and significantly increased expression of Zip1. In contrast, the silencing of Zip1 expression reversed MLT-mediated fracture healing. In summary, Zip1 participated in the regulation of the NPY/NPY1R signaling pathway via MLT to promote the osteogenic differentiation of MSCs and fracture healing.
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spelling pubmed-77315762021-11-09 Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats Shang, Junjun Ma, Bin Zhu, Guiling Dong, Penghong Wang, Chan Gu, Xiaochuan Zi, Ying Eur J Histochem Article Our previous study documented that melatonin (MLT) induced the osteogenic differentiation of mesenchymal stem cells (MSCs) and promoted the healing of femoral fractures in rats via the neuropeptide Y (NPY)/neuropeptide Y1 receptor (NPY1R) signaling pathway. MLT treatment upregulated the expression of the zinc uptake transporter zinc transporter 1 (Zip1) in nerve cells. Prior research demonstrated that oral zinc upregulated NPY expression. MSCs were isolated from rat bone marrow and identified using flow cytometry in our study. The results showed that MLT treatment upregulated NPY and NPY1R levels in MSCs with osteogenic differentiation, which was accompanied by upregulated Zip1 expression. However, the MLT-induced osteogenic differentiation of MSCs was reversed after interference of Zip1 expression. It was confirmed by the decreased alkaline phosphatase (ALP) level; downregulated activities of type I collagen α1 chain (COL1A1), osteocalcin (OCN), runt-related transcription factor 2 (Runx2) and ALP; and reduced mineralized nodule formation. MLT promoted fracture healing in rats with femoral fracture, which was accompanied by increased expression of NPY and NPY1R and significantly increased expression of Zip1. In contrast, the silencing of Zip1 expression reversed MLT-mediated fracture healing. In summary, Zip1 participated in the regulation of the NPY/NPY1R signaling pathway via MLT to promote the osteogenic differentiation of MSCs and fracture healing. PAGEPress Publications, Pavia, Italy 2020-12-02 /pmc/articles/PMC7731576/ /pubmed/33334091 http://dx.doi.org/10.4081/ejh.2020.3183 Text en ©Copyright: the Author(s) https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution NonCommercial 4.0 License (CC BY-NC 4.0).
spellingShingle Article
Shang, Junjun
Ma, Bin
Zhu, Guiling
Dong, Penghong
Wang, Chan
Gu, Xiaochuan
Zi, Ying
Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats
title Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats
title_full Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats
title_fullStr Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats
title_full_unstemmed Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats
title_short Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats
title_sort role of zip1 in the regulation of npy expression by mlt to promote fracture healing in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731576/
https://www.ncbi.nlm.nih.gov/pubmed/33334091
http://dx.doi.org/10.4081/ejh.2020.3183
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