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Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models
BACKGROUND AND AIM: Daikenchuto (DKT), a traditional Japanese formula, comprises four herbal medicines and is used for abdominal pain. Inflammatory bowel disease (IBD) includes ulcerative colitis (UC) and Crohn's disease (CD) and is characterized by colonic inflammation and chronic abdominal pa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wiley Publishing Asia Pty Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731802/ https://www.ncbi.nlm.nih.gov/pubmed/33319050 http://dx.doi.org/10.1002/jgh3.12410 |
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author | Kogure, Yoko Kanda, Hirosato Wang, Shenglan Hao, Yongbiao Li, Junxiang Yamamoto, Satoshi Noguchi, Koichi Dai, Yi |
author_facet | Kogure, Yoko Kanda, Hirosato Wang, Shenglan Hao, Yongbiao Li, Junxiang Yamamoto, Satoshi Noguchi, Koichi Dai, Yi |
author_sort | Kogure, Yoko |
collection | PubMed |
description | BACKGROUND AND AIM: Daikenchuto (DKT), a traditional Japanese formula, comprises four herbal medicines and is used for abdominal pain. Inflammatory bowel disease (IBD) includes ulcerative colitis (UC) and Crohn's disease (CD) and is characterized by colonic inflammation and chronic abdominal pain. The present study aimed to investigate whether DKT suppresses colonic hypersensitivity and inflammation associated with IBD in animal models. METHODS: Sprague–Dawley rats were administered 4% sodium dextran sulfate (DSS) or trinitrobenzene sulfate (TNBS) in the colon to establish UC or CD models, respectively. DKT and 5‐aminosalicylic acid (5‐ASA) were administered orally once a day from Days 3 to 7 after induction of colitis. On Day 7, visceral pain and inflammation were evaluated by measuring the visceromotor response (VMR) to colorectal distention (CRD) and inflammatory indicators, including histological score, length of leukocyte infiltration, MPO activity, and eosinophil count. RESULTS: DSS and TNBS increased VMR to CRD and the inflammation indicators. DKT, but not 5‐ASA, suppressed the VMR to CRD in DSS‐ and TNBS‐treated rats. DKT and 5‐ASA decreased the eosinophil count in both IBD models. In DSS‐treated rats, 5‐ASA, but not DKT, suppressed the MPO activity. In TNBS‐treated rats, neither 5‐ASA nor DKT suppressed MPO activity. CONCLUSION: These results suggest that DKT is beneficial for abdominal pain associated with IBD. The anti‐inflammatory effect of DKT on IBD may involve inhibition of eosinophils. The mechanism of anti‐inflammatory effect of DKT partially differs from that of 5‐ASA. Coapplication of DKT and conventional medicine may produce a positive synergy effect for IBD treatment. |
format | Online Article Text |
id | pubmed-7731802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wiley Publishing Asia Pty Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-77318022020-12-13 Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models Kogure, Yoko Kanda, Hirosato Wang, Shenglan Hao, Yongbiao Li, Junxiang Yamamoto, Satoshi Noguchi, Koichi Dai, Yi JGH Open Original Articles BACKGROUND AND AIM: Daikenchuto (DKT), a traditional Japanese formula, comprises four herbal medicines and is used for abdominal pain. Inflammatory bowel disease (IBD) includes ulcerative colitis (UC) and Crohn's disease (CD) and is characterized by colonic inflammation and chronic abdominal pain. The present study aimed to investigate whether DKT suppresses colonic hypersensitivity and inflammation associated with IBD in animal models. METHODS: Sprague–Dawley rats were administered 4% sodium dextran sulfate (DSS) or trinitrobenzene sulfate (TNBS) in the colon to establish UC or CD models, respectively. DKT and 5‐aminosalicylic acid (5‐ASA) were administered orally once a day from Days 3 to 7 after induction of colitis. On Day 7, visceral pain and inflammation were evaluated by measuring the visceromotor response (VMR) to colorectal distention (CRD) and inflammatory indicators, including histological score, length of leukocyte infiltration, MPO activity, and eosinophil count. RESULTS: DSS and TNBS increased VMR to CRD and the inflammation indicators. DKT, but not 5‐ASA, suppressed the VMR to CRD in DSS‐ and TNBS‐treated rats. DKT and 5‐ASA decreased the eosinophil count in both IBD models. In DSS‐treated rats, 5‐ASA, but not DKT, suppressed the MPO activity. In TNBS‐treated rats, neither 5‐ASA nor DKT suppressed MPO activity. CONCLUSION: These results suggest that DKT is beneficial for abdominal pain associated with IBD. The anti‐inflammatory effect of DKT on IBD may involve inhibition of eosinophils. The mechanism of anti‐inflammatory effect of DKT partially differs from that of 5‐ASA. Coapplication of DKT and conventional medicine may produce a positive synergy effect for IBD treatment. Wiley Publishing Asia Pty Ltd 2020-08-22 /pmc/articles/PMC7731802/ /pubmed/33319050 http://dx.doi.org/10.1002/jgh3.12410 Text en © 2020 The Authors. JGH Open published by Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Kogure, Yoko Kanda, Hirosato Wang, Shenglan Hao, Yongbiao Li, Junxiang Yamamoto, Satoshi Noguchi, Koichi Dai, Yi Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models |
title | Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models |
title_full | Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models |
title_fullStr | Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models |
title_full_unstemmed | Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models |
title_short | Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models |
title_sort | daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731802/ https://www.ncbi.nlm.nih.gov/pubmed/33319050 http://dx.doi.org/10.1002/jgh3.12410 |
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