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Ancestry of the AUTS2 family–A novel group of polycomb-complex proteins involved in human neurological disease

Autism susceptibility candidate 2 (AUTS2) is a neurodevelopmental regulator associated with an autosomal dominant intellectual disability syndrome, AUTS2 syndrome, and is implicated as an important gene in human-specific evolution. AUTS2 exists as part of a tripartite gene family, the AUTS2 family,...

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Autores principales: Sellers, Robert A., Robertson, David L., Tassabehji, May
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732068/
https://www.ncbi.nlm.nih.gov/pubmed/33306672
http://dx.doi.org/10.1371/journal.pone.0232101
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author Sellers, Robert A.
Robertson, David L.
Tassabehji, May
author_facet Sellers, Robert A.
Robertson, David L.
Tassabehji, May
author_sort Sellers, Robert A.
collection PubMed
description Autism susceptibility candidate 2 (AUTS2) is a neurodevelopmental regulator associated with an autosomal dominant intellectual disability syndrome, AUTS2 syndrome, and is implicated as an important gene in human-specific evolution. AUTS2 exists as part of a tripartite gene family, the AUTS2 family, which includes two relatively undefined proteins, Fibrosin (FBRS) and Fibrosin-like protein 1 (FBRSL1). Evolutionary ancestors of AUTS2 have not been formally identified outside of the Animalia clade. A Drosophila melanogaster protein, Tay bridge, with a role in neurodevelopment, has been shown to display limited similarity to the C-terminal of AUTS2, suggesting that evolutionary ancestors of the AUTS2 family may exist within other Protostome lineages. Here we present an evolutionary analysis of the AUTS2 family, which highlights ancestral homologs of AUTS2 in multiple Protostome species, implicates AUTS2 as the closest human relative to the progenitor of the AUTS2 family, and demonstrates that Tay bridge is a divergent ortholog of the ancestral AUTS2 progenitor gene. We also define regions of high relative sequence identity, with potential functional significance, shared by the extended AUTS2 protein family. Using structural predictions coupled with sequence conservation and human variant data from 15,708 individuals, a putative domain structure for AUTS2 was produced that can be used to aid interpretation of the consequences of nucleotide variation on protein structure and function in human disease. To assess the role of AUTS2 in human-specific evolution, we recalculated allele frequencies at previously identified human derived sites using large population genome data, and show a high prevalence of ancestral alleles, suggesting that AUTS2 may not be a rapidly evolving gene, as previously thought.
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spelling pubmed-77320682020-12-17 Ancestry of the AUTS2 family–A novel group of polycomb-complex proteins involved in human neurological disease Sellers, Robert A. Robertson, David L. Tassabehji, May PLoS One Research Article Autism susceptibility candidate 2 (AUTS2) is a neurodevelopmental regulator associated with an autosomal dominant intellectual disability syndrome, AUTS2 syndrome, and is implicated as an important gene in human-specific evolution. AUTS2 exists as part of a tripartite gene family, the AUTS2 family, which includes two relatively undefined proteins, Fibrosin (FBRS) and Fibrosin-like protein 1 (FBRSL1). Evolutionary ancestors of AUTS2 have not been formally identified outside of the Animalia clade. A Drosophila melanogaster protein, Tay bridge, with a role in neurodevelopment, has been shown to display limited similarity to the C-terminal of AUTS2, suggesting that evolutionary ancestors of the AUTS2 family may exist within other Protostome lineages. Here we present an evolutionary analysis of the AUTS2 family, which highlights ancestral homologs of AUTS2 in multiple Protostome species, implicates AUTS2 as the closest human relative to the progenitor of the AUTS2 family, and demonstrates that Tay bridge is a divergent ortholog of the ancestral AUTS2 progenitor gene. We also define regions of high relative sequence identity, with potential functional significance, shared by the extended AUTS2 protein family. Using structural predictions coupled with sequence conservation and human variant data from 15,708 individuals, a putative domain structure for AUTS2 was produced that can be used to aid interpretation of the consequences of nucleotide variation on protein structure and function in human disease. To assess the role of AUTS2 in human-specific evolution, we recalculated allele frequencies at previously identified human derived sites using large population genome data, and show a high prevalence of ancestral alleles, suggesting that AUTS2 may not be a rapidly evolving gene, as previously thought. Public Library of Science 2020-12-11 /pmc/articles/PMC7732068/ /pubmed/33306672 http://dx.doi.org/10.1371/journal.pone.0232101 Text en © 2020 Sellers et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sellers, Robert A.
Robertson, David L.
Tassabehji, May
Ancestry of the AUTS2 family–A novel group of polycomb-complex proteins involved in human neurological disease
title Ancestry of the AUTS2 family–A novel group of polycomb-complex proteins involved in human neurological disease
title_full Ancestry of the AUTS2 family–A novel group of polycomb-complex proteins involved in human neurological disease
title_fullStr Ancestry of the AUTS2 family–A novel group of polycomb-complex proteins involved in human neurological disease
title_full_unstemmed Ancestry of the AUTS2 family–A novel group of polycomb-complex proteins involved in human neurological disease
title_short Ancestry of the AUTS2 family–A novel group of polycomb-complex proteins involved in human neurological disease
title_sort ancestry of the auts2 family–a novel group of polycomb-complex proteins involved in human neurological disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732068/
https://www.ncbi.nlm.nih.gov/pubmed/33306672
http://dx.doi.org/10.1371/journal.pone.0232101
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