Cargando…

Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system

Pathogen identification is a critical step during diagnosis of infectious diseases. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight mass spectrometry (MALDI-TOF-MS) has become the gold standard for identification of microorganisms cultured on solid media in microbiology laboratories. Dire...

Descripción completa

Detalles Bibliográficos
Autores principales: Noll, Christine, Nasruddin-Yekta, Azadda, Sternisek, Pia, Weig, Michael, Groß, Uwe, Schilling, Arndt F., Beil, Frank Timo, Bader, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732097/
https://www.ncbi.nlm.nih.gov/pubmed/33306699
http://dx.doi.org/10.1371/journal.pone.0243790
_version_ 1783622020498456576
author Noll, Christine
Nasruddin-Yekta, Azadda
Sternisek, Pia
Weig, Michael
Groß, Uwe
Schilling, Arndt F.
Beil, Frank Timo
Bader, Oliver
author_facet Noll, Christine
Nasruddin-Yekta, Azadda
Sternisek, Pia
Weig, Michael
Groß, Uwe
Schilling, Arndt F.
Beil, Frank Timo
Bader, Oliver
author_sort Noll, Christine
collection PubMed
description Pathogen identification is a critical step during diagnosis of infectious diseases. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight mass spectrometry (MALDI-TOF-MS) has become the gold standard for identification of microorganisms cultured on solid media in microbiology laboratories. Direct identification of microbes from liquid specimen, circumventing the need for the additional overnight cultivation step, has been successfully established for blood culture, urine and liquor. Here, we evaluate the ability of MALDI-TOF MS for direct identification of pathogens in synovial fluid after liquid enrichment in BacT/Alert blood culture bottles. Influence of synovial specimen quality on direct species identification with the MALDI BioTyper/Sepsityper was tested with samples inoculated from pretested native synovia with concomitant inoculation of blood or pus, or highly viscous fluid. Here, we achieved >90% concordance with culture on solid medium, and only mixed-species samples posed significant problems. Performance in routine diagnostics was tested prospectively on bottles inoculated by treating physicians on ward. There, we achieved >70% concordance with culture on solid media. The major contributors to test failure were the absence of a measurable mass signal and mixed-specimen samples. The Sepsityper workflow worked well on samples derived from BacT/Alert blood culture bottles inoculated with synovial fluid, giving concordant results to identification from solid media. Host remnant material in the inoculum, such as blood or pus, had no detrimental effect on identification score values of the BioTyper system after processing with the Sepsityper workflow, and neither had the initial viscosity of the synovial sample.
format Online
Article
Text
id pubmed-7732097
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-77320972020-12-17 Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system Noll, Christine Nasruddin-Yekta, Azadda Sternisek, Pia Weig, Michael Groß, Uwe Schilling, Arndt F. Beil, Frank Timo Bader, Oliver PLoS One Research Article Pathogen identification is a critical step during diagnosis of infectious diseases. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight mass spectrometry (MALDI-TOF-MS) has become the gold standard for identification of microorganisms cultured on solid media in microbiology laboratories. Direct identification of microbes from liquid specimen, circumventing the need for the additional overnight cultivation step, has been successfully established for blood culture, urine and liquor. Here, we evaluate the ability of MALDI-TOF MS for direct identification of pathogens in synovial fluid after liquid enrichment in BacT/Alert blood culture bottles. Influence of synovial specimen quality on direct species identification with the MALDI BioTyper/Sepsityper was tested with samples inoculated from pretested native synovia with concomitant inoculation of blood or pus, or highly viscous fluid. Here, we achieved >90% concordance with culture on solid medium, and only mixed-species samples posed significant problems. Performance in routine diagnostics was tested prospectively on bottles inoculated by treating physicians on ward. There, we achieved >70% concordance with culture on solid media. The major contributors to test failure were the absence of a measurable mass signal and mixed-specimen samples. The Sepsityper workflow worked well on samples derived from BacT/Alert blood culture bottles inoculated with synovial fluid, giving concordant results to identification from solid media. Host remnant material in the inoculum, such as blood or pus, had no detrimental effect on identification score values of the BioTyper system after processing with the Sepsityper workflow, and neither had the initial viscosity of the synovial sample. Public Library of Science 2020-12-11 /pmc/articles/PMC7732097/ /pubmed/33306699 http://dx.doi.org/10.1371/journal.pone.0243790 Text en © 2020 Noll et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Noll, Christine
Nasruddin-Yekta, Azadda
Sternisek, Pia
Weig, Michael
Groß, Uwe
Schilling, Arndt F.
Beil, Frank Timo
Bader, Oliver
Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system
title Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system
title_full Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system
title_fullStr Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system
title_full_unstemmed Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system
title_short Rapid direct detection of pathogens for diagnosis of joint infections by MALDI-TOF MS after liquid enrichment in the BacT/Alert blood culture system
title_sort rapid direct detection of pathogens for diagnosis of joint infections by maldi-tof ms after liquid enrichment in the bact/alert blood culture system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732097/
https://www.ncbi.nlm.nih.gov/pubmed/33306699
http://dx.doi.org/10.1371/journal.pone.0243790
work_keys_str_mv AT nollchristine rapiddirectdetectionofpathogensfordiagnosisofjointinfectionsbymalditofmsafterliquidenrichmentinthebactalertbloodculturesystem
AT nasruddinyektaazadda rapiddirectdetectionofpathogensfordiagnosisofjointinfectionsbymalditofmsafterliquidenrichmentinthebactalertbloodculturesystem
AT sternisekpia rapiddirectdetectionofpathogensfordiagnosisofjointinfectionsbymalditofmsafterliquidenrichmentinthebactalertbloodculturesystem
AT weigmichael rapiddirectdetectionofpathogensfordiagnosisofjointinfectionsbymalditofmsafterliquidenrichmentinthebactalertbloodculturesystem
AT großuwe rapiddirectdetectionofpathogensfordiagnosisofjointinfectionsbymalditofmsafterliquidenrichmentinthebactalertbloodculturesystem
AT schillingarndtf rapiddirectdetectionofpathogensfordiagnosisofjointinfectionsbymalditofmsafterliquidenrichmentinthebactalertbloodculturesystem
AT beilfranktimo rapiddirectdetectionofpathogensfordiagnosisofjointinfectionsbymalditofmsafterliquidenrichmentinthebactalertbloodculturesystem
AT baderoliver rapiddirectdetectionofpathogensfordiagnosisofjointinfectionsbymalditofmsafterliquidenrichmentinthebactalertbloodculturesystem