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Phytochemical Analysis, Cytotoxic, Antioxidant, and Antibacterial Activities of Lichens

BACKGROUND: Lichens present a complex symbiotic relationship between a filamentous fungus, photoautotrophic partner (algae or cyanobacteria), and bacterial community. The Objective of the Study. This study aimed at investigating the chemical composition and cytotoxic, antioxidant, and antimicrobial...

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Autores principales: Aoussar, Noura, Laasri, Fatima Ezzahra, Bourhia, Mohammed, Manoljovic, Nedeljko, Mhand, Rajaa Ait, Rhallabi, Naima, Ullah, Riaz, Shahat, Abdelaaty A., Noman, Omar M., Nasr, Fahd A, Almarfadi, Omer M., El Mzibri, Mohammed, Vasiljević, Perica, Benbacer, Laila, Mellouki, Fouad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732381/
https://www.ncbi.nlm.nih.gov/pubmed/33343680
http://dx.doi.org/10.1155/2020/8104538
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author Aoussar, Noura
Laasri, Fatima Ezzahra
Bourhia, Mohammed
Manoljovic, Nedeljko
Mhand, Rajaa Ait
Rhallabi, Naima
Ullah, Riaz
Shahat, Abdelaaty A.
Noman, Omar M.
Nasr, Fahd A
Almarfadi, Omer M.
El Mzibri, Mohammed
Vasiljević, Perica
Benbacer, Laila
Mellouki, Fouad
author_facet Aoussar, Noura
Laasri, Fatima Ezzahra
Bourhia, Mohammed
Manoljovic, Nedeljko
Mhand, Rajaa Ait
Rhallabi, Naima
Ullah, Riaz
Shahat, Abdelaaty A.
Noman, Omar M.
Nasr, Fahd A
Almarfadi, Omer M.
El Mzibri, Mohammed
Vasiljević, Perica
Benbacer, Laila
Mellouki, Fouad
author_sort Aoussar, Noura
collection PubMed
description BACKGROUND: Lichens present a complex symbiotic relationship between a filamentous fungus, photoautotrophic partner (algae or cyanobacteria), and bacterial community. The Objective of the Study. This study aimed at investigating the chemical composition and cytotoxic, antioxidant, and antimicrobial activities of acetone extracts of Moroccan Evernia prunastri (E. prunastri), Ramalina farinacea (R. farinacea), and Pseudevernia furfuracea (P. furfuracea). Materials and Methods. The phytochemical analysis was carried out by HPLC-UV. The cytotoxic effect was assessed on human prostate cancer (22RV1), human colon carcinoma (HT-29), human hepatocellular carcinoma (Hep-G2), and Hamster ovarian cancer (CHO) cells lines by WST1 assay. The antioxidant power was assessed by DPPH and FRAP assays. The antibacterial effect was obtained using the broth microdilution method. RESULTS: The findings of phytochemical analysis showed that the lichens studied possess interesting bioactive molecules such as physodalic acid, evernic acid, and usnic acid, as well as protocetraric acid. According to the American National Cancer Institute guidelines, the WST-1 test showed that all crude extracts did not show significant cytotoxic effects against all concerous cell lines, and IC(50) values ranged from 42.30 to 140.24 µg/mL. Regarding the antioxidant activity, P. furfuracea extract showed the highest free-radical-scavenging ability (IC(50) = 498.40 µg/mL). The most potent antibacterial extract was recorded for P. furfuracea extract with a minimum inhibitory concentration (MIC) ranging from 0.039 to 0.31 mg/mL. CONCLUSION: In this research work, we report that the studied lichen extracts exhibit an important biological effect, supporting that lichens represent a hopeful source of original natural products for the research of new bioactive molecules having a pharmaceutical interest.
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spelling pubmed-77323812020-12-18 Phytochemical Analysis, Cytotoxic, Antioxidant, and Antibacterial Activities of Lichens Aoussar, Noura Laasri, Fatima Ezzahra Bourhia, Mohammed Manoljovic, Nedeljko Mhand, Rajaa Ait Rhallabi, Naima Ullah, Riaz Shahat, Abdelaaty A. Noman, Omar M. Nasr, Fahd A Almarfadi, Omer M. El Mzibri, Mohammed Vasiljević, Perica Benbacer, Laila Mellouki, Fouad Evid Based Complement Alternat Med Research Article BACKGROUND: Lichens present a complex symbiotic relationship between a filamentous fungus, photoautotrophic partner (algae or cyanobacteria), and bacterial community. The Objective of the Study. This study aimed at investigating the chemical composition and cytotoxic, antioxidant, and antimicrobial activities of acetone extracts of Moroccan Evernia prunastri (E. prunastri), Ramalina farinacea (R. farinacea), and Pseudevernia furfuracea (P. furfuracea). Materials and Methods. The phytochemical analysis was carried out by HPLC-UV. The cytotoxic effect was assessed on human prostate cancer (22RV1), human colon carcinoma (HT-29), human hepatocellular carcinoma (Hep-G2), and Hamster ovarian cancer (CHO) cells lines by WST1 assay. The antioxidant power was assessed by DPPH and FRAP assays. The antibacterial effect was obtained using the broth microdilution method. RESULTS: The findings of phytochemical analysis showed that the lichens studied possess interesting bioactive molecules such as physodalic acid, evernic acid, and usnic acid, as well as protocetraric acid. According to the American National Cancer Institute guidelines, the WST-1 test showed that all crude extracts did not show significant cytotoxic effects against all concerous cell lines, and IC(50) values ranged from 42.30 to 140.24 µg/mL. Regarding the antioxidant activity, P. furfuracea extract showed the highest free-radical-scavenging ability (IC(50) = 498.40 µg/mL). The most potent antibacterial extract was recorded for P. furfuracea extract with a minimum inhibitory concentration (MIC) ranging from 0.039 to 0.31 mg/mL. CONCLUSION: In this research work, we report that the studied lichen extracts exhibit an important biological effect, supporting that lichens represent a hopeful source of original natural products for the research of new bioactive molecules having a pharmaceutical interest. Hindawi 2020-12-02 /pmc/articles/PMC7732381/ /pubmed/33343680 http://dx.doi.org/10.1155/2020/8104538 Text en Copyright © 2020 Noura Aoussar et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Aoussar, Noura
Laasri, Fatima Ezzahra
Bourhia, Mohammed
Manoljovic, Nedeljko
Mhand, Rajaa Ait
Rhallabi, Naima
Ullah, Riaz
Shahat, Abdelaaty A.
Noman, Omar M.
Nasr, Fahd A
Almarfadi, Omer M.
El Mzibri, Mohammed
Vasiljević, Perica
Benbacer, Laila
Mellouki, Fouad
Phytochemical Analysis, Cytotoxic, Antioxidant, and Antibacterial Activities of Lichens
title Phytochemical Analysis, Cytotoxic, Antioxidant, and Antibacterial Activities of Lichens
title_full Phytochemical Analysis, Cytotoxic, Antioxidant, and Antibacterial Activities of Lichens
title_fullStr Phytochemical Analysis, Cytotoxic, Antioxidant, and Antibacterial Activities of Lichens
title_full_unstemmed Phytochemical Analysis, Cytotoxic, Antioxidant, and Antibacterial Activities of Lichens
title_short Phytochemical Analysis, Cytotoxic, Antioxidant, and Antibacterial Activities of Lichens
title_sort phytochemical analysis, cytotoxic, antioxidant, and antibacterial activities of lichens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732381/
https://www.ncbi.nlm.nih.gov/pubmed/33343680
http://dx.doi.org/10.1155/2020/8104538
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