Cargando…
The Crosstalk between Calcium Ions and Aldosterone Contributes to Inflammation, Apoptosis, and Calcification of VSMC via the AIF-1/NF-κB Pathway in Uremia
Vascular calcification is a major complication of maintenance hemodialysis patients. Studies have confirmed that calcification mainly occurs in the vascular smooth muscle cells (VSMC) of the vascular media. However, the exact pathogenesis of VSMC calcification is still unknown. This study shows that...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732390/ https://www.ncbi.nlm.nih.gov/pubmed/33343805 http://dx.doi.org/10.1155/2020/3431597 |
_version_ | 1783622083260973056 |
---|---|
author | Hao, Jianbing Tang, Jie Zhang, Lei Li, Xin Hao, Lirong |
author_facet | Hao, Jianbing Tang, Jie Zhang, Lei Li, Xin Hao, Lirong |
author_sort | Hao, Jianbing |
collection | PubMed |
description | Vascular calcification is a major complication of maintenance hemodialysis patients. Studies have confirmed that calcification mainly occurs in the vascular smooth muscle cells (VSMC) of the vascular media. However, the exact pathogenesis of VSMC calcification is still unknown. This study shows that the crosstalk between calcium and aldosterone via the allograft inflammatory factor 1 (AIF-1) pathway contributes to calcium homeostasis and VSMC calcification, which is a novel mechanism of vascular calcification in uremia. In vivo results showed that the level of aldosterone and inflammatory factors increased in calcified arteries, whereas no significant changes were observed in peripheral blood. However, the expression of inflammatory factors markedly increased in the peripheral blood of uremic rats without aortic calcification and gradually returned to normal levels with aggravation of aortic calcification. In vitro results showed that there was an interaction between calcium ions and aldosterone in macrophages or VSMC. Calcium induced aldosterone synthesis, and in turn, aldosterone also triggered intracellular calcium content upregulation in macrophages or VSMC. Furthermore, activated macrophages induced inflammation, apoptosis, and calcification of VSMC. Activated VSMC also imparted a similar effect on untreated VSMC. Finally, AIF-1 enhanced aldosterone- or calcium-induced VSMC calcification, and NF-κB inhibitors inhibited the effect of AIF-1 on VSMC. These in vivo and in vitro results suggest that the crosstalk between calcium ions and aldosterone plays an important role in VSMC calcification in uremia via the AIF-1/NF-κB pathway. Local calcified VSMC induced the same pathological process in surrounding VSMC, thereby contributing to calcium homeostasis and accelerating vascular calcification. |
format | Online Article Text |
id | pubmed-7732390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-77323902020-12-18 The Crosstalk between Calcium Ions and Aldosterone Contributes to Inflammation, Apoptosis, and Calcification of VSMC via the AIF-1/NF-κB Pathway in Uremia Hao, Jianbing Tang, Jie Zhang, Lei Li, Xin Hao, Lirong Oxid Med Cell Longev Research Article Vascular calcification is a major complication of maintenance hemodialysis patients. Studies have confirmed that calcification mainly occurs in the vascular smooth muscle cells (VSMC) of the vascular media. However, the exact pathogenesis of VSMC calcification is still unknown. This study shows that the crosstalk between calcium and aldosterone via the allograft inflammatory factor 1 (AIF-1) pathway contributes to calcium homeostasis and VSMC calcification, which is a novel mechanism of vascular calcification in uremia. In vivo results showed that the level of aldosterone and inflammatory factors increased in calcified arteries, whereas no significant changes were observed in peripheral blood. However, the expression of inflammatory factors markedly increased in the peripheral blood of uremic rats without aortic calcification and gradually returned to normal levels with aggravation of aortic calcification. In vitro results showed that there was an interaction between calcium ions and aldosterone in macrophages or VSMC. Calcium induced aldosterone synthesis, and in turn, aldosterone also triggered intracellular calcium content upregulation in macrophages or VSMC. Furthermore, activated macrophages induced inflammation, apoptosis, and calcification of VSMC. Activated VSMC also imparted a similar effect on untreated VSMC. Finally, AIF-1 enhanced aldosterone- or calcium-induced VSMC calcification, and NF-κB inhibitors inhibited the effect of AIF-1 on VSMC. These in vivo and in vitro results suggest that the crosstalk between calcium ions and aldosterone plays an important role in VSMC calcification in uremia via the AIF-1/NF-κB pathway. Local calcified VSMC induced the same pathological process in surrounding VSMC, thereby contributing to calcium homeostasis and accelerating vascular calcification. Hindawi 2020-12-04 /pmc/articles/PMC7732390/ /pubmed/33343805 http://dx.doi.org/10.1155/2020/3431597 Text en Copyright © 2020 Jianbing Hao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hao, Jianbing Tang, Jie Zhang, Lei Li, Xin Hao, Lirong The Crosstalk between Calcium Ions and Aldosterone Contributes to Inflammation, Apoptosis, and Calcification of VSMC via the AIF-1/NF-κB Pathway in Uremia |
title | The Crosstalk between Calcium Ions and Aldosterone Contributes to Inflammation, Apoptosis, and Calcification of VSMC via the AIF-1/NF-κB Pathway in Uremia |
title_full | The Crosstalk between Calcium Ions and Aldosterone Contributes to Inflammation, Apoptosis, and Calcification of VSMC via the AIF-1/NF-κB Pathway in Uremia |
title_fullStr | The Crosstalk between Calcium Ions and Aldosterone Contributes to Inflammation, Apoptosis, and Calcification of VSMC via the AIF-1/NF-κB Pathway in Uremia |
title_full_unstemmed | The Crosstalk between Calcium Ions and Aldosterone Contributes to Inflammation, Apoptosis, and Calcification of VSMC via the AIF-1/NF-κB Pathway in Uremia |
title_short | The Crosstalk between Calcium Ions and Aldosterone Contributes to Inflammation, Apoptosis, and Calcification of VSMC via the AIF-1/NF-κB Pathway in Uremia |
title_sort | crosstalk between calcium ions and aldosterone contributes to inflammation, apoptosis, and calcification of vsmc via the aif-1/nf-κb pathway in uremia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732390/ https://www.ncbi.nlm.nih.gov/pubmed/33343805 http://dx.doi.org/10.1155/2020/3431597 |
work_keys_str_mv | AT haojianbing thecrosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT tangjie thecrosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT zhanglei thecrosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT lixin thecrosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT haolirong thecrosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT haojianbing crosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT tangjie crosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT zhanglei crosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT lixin crosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia AT haolirong crosstalkbetweencalciumionsandaldosteronecontributestoinflammationapoptosisandcalcificationofvsmcviatheaif1nfkbpathwayinuremia |