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The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato

Nitrate is an essential macronutrient and a signal molecule that regulates the expression of multiple genes involved in plant growth and development. Here, we describe the participation of Arabidopsis DNA binding with one finger (DOF) transcription factor CDF3 in nitrate responses and shows that CDF...

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Autores principales: Domínguez-Figueroa, José, Carrillo, Laura, Renau-Morata, Begoña, Yang, Lu, Molina, Rosa-V, Marino, Daniel, Canales, Javier, Weih, Martin, Vicente-Carbajosa, Jesús, Nebauer, Sergio G., Medina, Joaquín
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732579/
https://www.ncbi.nlm.nih.gov/pubmed/33329669
http://dx.doi.org/10.3389/fpls.2020.601558
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author Domínguez-Figueroa, José
Carrillo, Laura
Renau-Morata, Begoña
Yang, Lu
Molina, Rosa-V
Marino, Daniel
Canales, Javier
Weih, Martin
Vicente-Carbajosa, Jesús
Nebauer, Sergio G.
Medina, Joaquín
author_facet Domínguez-Figueroa, José
Carrillo, Laura
Renau-Morata, Begoña
Yang, Lu
Molina, Rosa-V
Marino, Daniel
Canales, Javier
Weih, Martin
Vicente-Carbajosa, Jesús
Nebauer, Sergio G.
Medina, Joaquín
author_sort Domínguez-Figueroa, José
collection PubMed
description Nitrate is an essential macronutrient and a signal molecule that regulates the expression of multiple genes involved in plant growth and development. Here, we describe the participation of Arabidopsis DNA binding with one finger (DOF) transcription factor CDF3 in nitrate responses and shows that CDF3 gene is induced under nitrate starvation. Moreover, knockout cdf3 mutant plants exhibit nitrate-dependent lateral and primary root modifications, whereas CDF3 overexpression plants show increased biomass and enhanced root development under both nitrogen poor and rich conditions. Expression analyses of 35S::CDF3 lines reveled that CDF3 regulates the expression of an important set of nitrate responsive genes including, glutamine synthetase-1, glutamate synthase-2, nitrate reductase-1, and nitrate transporters NRT2.1, NRT2.4, and NRT2.5 as well as carbon assimilation genes like PK1 and PEPC1 in response to N availability. Consistently, metabolite profiling disclosed that the total amount of key N metabolites like glutamate, glutamine, and asparagine were higher in CDF3-overexpressing plants, but lower in cdf3-1 in N limiting conditions. Moreover, overexpression of CDF3 in tomato increased N accumulation and yield efficiency under both optimum and limiting N supply. These results highlight CDF3 as an important regulatory factor for the nitrate response, and its potential for improving N use efficiency in crops.
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spelling pubmed-77325792020-12-15 The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato Domínguez-Figueroa, José Carrillo, Laura Renau-Morata, Begoña Yang, Lu Molina, Rosa-V Marino, Daniel Canales, Javier Weih, Martin Vicente-Carbajosa, Jesús Nebauer, Sergio G. Medina, Joaquín Front Plant Sci Plant Science Nitrate is an essential macronutrient and a signal molecule that regulates the expression of multiple genes involved in plant growth and development. Here, we describe the participation of Arabidopsis DNA binding with one finger (DOF) transcription factor CDF3 in nitrate responses and shows that CDF3 gene is induced under nitrate starvation. Moreover, knockout cdf3 mutant plants exhibit nitrate-dependent lateral and primary root modifications, whereas CDF3 overexpression plants show increased biomass and enhanced root development under both nitrogen poor and rich conditions. Expression analyses of 35S::CDF3 lines reveled that CDF3 regulates the expression of an important set of nitrate responsive genes including, glutamine synthetase-1, glutamate synthase-2, nitrate reductase-1, and nitrate transporters NRT2.1, NRT2.4, and NRT2.5 as well as carbon assimilation genes like PK1 and PEPC1 in response to N availability. Consistently, metabolite profiling disclosed that the total amount of key N metabolites like glutamate, glutamine, and asparagine were higher in CDF3-overexpressing plants, but lower in cdf3-1 in N limiting conditions. Moreover, overexpression of CDF3 in tomato increased N accumulation and yield efficiency under both optimum and limiting N supply. These results highlight CDF3 as an important regulatory factor for the nitrate response, and its potential for improving N use efficiency in crops. Frontiers Media S.A. 2020-11-24 /pmc/articles/PMC7732579/ /pubmed/33329669 http://dx.doi.org/10.3389/fpls.2020.601558 Text en Copyright © 2020 Domínguez-Figueroa, Carrillo, Renau-Morata, Yang, Molina, Marino, Canales, Weih, Vicente-Carbajosa, Nebauer and Medina. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Domínguez-Figueroa, José
Carrillo, Laura
Renau-Morata, Begoña
Yang, Lu
Molina, Rosa-V
Marino, Daniel
Canales, Javier
Weih, Martin
Vicente-Carbajosa, Jesús
Nebauer, Sergio G.
Medina, Joaquín
The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato
title The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato
title_full The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato
title_fullStr The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato
title_full_unstemmed The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato
title_short The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato
title_sort arabidopsis transcription factor cdf3 is involved in nitrogen responses and improves nitrogen use efficiency in tomato
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732579/
https://www.ncbi.nlm.nih.gov/pubmed/33329669
http://dx.doi.org/10.3389/fpls.2020.601558
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