High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli

Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anteri...

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Autores principales: Liu, Bing Yan, Gao, Bin, Liu, Meng Zhi, Zhang, Ting Ting, Liu, Bao Shan, Chen, Ze Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732928/
https://www.ncbi.nlm.nih.gov/pubmed/33306160
http://dx.doi.org/10.1186/s13568-020-01163-8
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author Liu, Bing Yan
Gao, Bin
Liu, Meng Zhi
Zhang, Ting Ting
Liu, Bao Shan
Chen, Ze Liang
author_facet Liu, Bing Yan
Gao, Bin
Liu, Meng Zhi
Zhang, Ting Ting
Liu, Bao Shan
Chen, Ze Liang
author_sort Liu, Bing Yan
collection PubMed
description Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein.
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spelling pubmed-77329282020-12-21 High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli Liu, Bing Yan Gao, Bin Liu, Meng Zhi Zhang, Ting Ting Liu, Bao Shan Chen, Ze Liang AMB Express Original Article Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein. Springer Berlin Heidelberg 2020-12-11 /pmc/articles/PMC7732928/ /pubmed/33306160 http://dx.doi.org/10.1186/s13568-020-01163-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Liu, Bing Yan
Gao, Bin
Liu, Meng Zhi
Zhang, Ting Ting
Liu, Bao Shan
Chen, Ze Liang
High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli
title High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli
title_full High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli
title_fullStr High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli
title_full_unstemmed High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli
title_short High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli
title_sort high repetitive arginine in the anterior of pcv3 capsid protein is a severe obstacle for its expression in e. coli
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732928/
https://www.ncbi.nlm.nih.gov/pubmed/33306160
http://dx.doi.org/10.1186/s13568-020-01163-8
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