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The TOP vector: a new high-titer lentiviral construct for delivery of sgRNAs and transgenes to primary T cells
Efficient delivery of nucleic acids for the engineering of primary T cells is central to the study of the basic biology of these key immune effector cells and has clinical implications. To date, lentiviral vectors delivering guide RNAs for CRISPR-Cas9 editing are not optimal for use in primary cells...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society of Gene & Cell Therapy
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732963/ https://www.ncbi.nlm.nih.gov/pubmed/33335945 http://dx.doi.org/10.1016/j.omtm.2020.10.020 |