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Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor

This article presents the construction of a multimodality platform that can be used for efficient destruction of brain tumor by a combination of photodynamic and sonodynamic therapy. For in vivo studies, U87 patient-derived xenograft tumors were implanted subcutaneously in SCID mice. For the first t...

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Autores principales: Borah, Ballav M., Cacaccio, Joseph, Durrani, Farukh A., Bshara, Wiam, Turowski, Steven G., Spernyak, Joseph A., Pandey, Ravindra K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732989/
https://www.ncbi.nlm.nih.gov/pubmed/33311561
http://dx.doi.org/10.1038/s41598-020-78153-0
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author Borah, Ballav M.
Cacaccio, Joseph
Durrani, Farukh A.
Bshara, Wiam
Turowski, Steven G.
Spernyak, Joseph A.
Pandey, Ravindra K.
author_facet Borah, Ballav M.
Cacaccio, Joseph
Durrani, Farukh A.
Bshara, Wiam
Turowski, Steven G.
Spernyak, Joseph A.
Pandey, Ravindra K.
author_sort Borah, Ballav M.
collection PubMed
description This article presents the construction of a multimodality platform that can be used for efficient destruction of brain tumor by a combination of photodynamic and sonodynamic therapy. For in vivo studies, U87 patient-derived xenograft tumors were implanted subcutaneously in SCID mice. For the first time, it has been shown that the cell-death mechanism by both treatment modalities follows two different pathways. For example, exposing the U87 cells after 24 h incubation with HPPH [3-(1′-hexyloxy)ethyl-3-devinyl-pyropheophorbide-a) by ultrasound participate in an electron-transfer process with the surrounding biological substrates to form radicals and radical ions (Type I reaction); whereas in photodynamic therapy, the tumor destruction is mainly caused by highly reactive singlet oxygen (Type II reaction). The combination of photodynamic therapy and sonodynamic therapy both in vitro and in vivo have shown an improved cell kill/tumor response, that could be attributed to an additive and/or synergetic effect(s). Our results also indicate that the delivery of the HPPH to tumors can further be enhanced by using cationic polyacrylamide nanoparticles as a delivery vehicle. Exposing the nano-formulation with ultrasound also triggered the release of photosensitizer. The combination of photodynamic therapy and sonodynamic therapy strongly affects tumor vasculature as determined by dynamic contrast enhanced imaging using HSA-Gd(III)DTPA.
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spelling pubmed-77329892020-12-15 Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor Borah, Ballav M. Cacaccio, Joseph Durrani, Farukh A. Bshara, Wiam Turowski, Steven G. Spernyak, Joseph A. Pandey, Ravindra K. Sci Rep Article This article presents the construction of a multimodality platform that can be used for efficient destruction of brain tumor by a combination of photodynamic and sonodynamic therapy. For in vivo studies, U87 patient-derived xenograft tumors were implanted subcutaneously in SCID mice. For the first time, it has been shown that the cell-death mechanism by both treatment modalities follows two different pathways. For example, exposing the U87 cells after 24 h incubation with HPPH [3-(1′-hexyloxy)ethyl-3-devinyl-pyropheophorbide-a) by ultrasound participate in an electron-transfer process with the surrounding biological substrates to form radicals and radical ions (Type I reaction); whereas in photodynamic therapy, the tumor destruction is mainly caused by highly reactive singlet oxygen (Type II reaction). The combination of photodynamic therapy and sonodynamic therapy both in vitro and in vivo have shown an improved cell kill/tumor response, that could be attributed to an additive and/or synergetic effect(s). Our results also indicate that the delivery of the HPPH to tumors can further be enhanced by using cationic polyacrylamide nanoparticles as a delivery vehicle. Exposing the nano-formulation with ultrasound also triggered the release of photosensitizer. The combination of photodynamic therapy and sonodynamic therapy strongly affects tumor vasculature as determined by dynamic contrast enhanced imaging using HSA-Gd(III)DTPA. Nature Publishing Group UK 2020-12-11 /pmc/articles/PMC7732989/ /pubmed/33311561 http://dx.doi.org/10.1038/s41598-020-78153-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Borah, Ballav M.
Cacaccio, Joseph
Durrani, Farukh A.
Bshara, Wiam
Turowski, Steven G.
Spernyak, Joseph A.
Pandey, Ravindra K.
Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor
title Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor
title_full Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor
title_fullStr Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor
title_full_unstemmed Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor
title_short Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor
title_sort sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732989/
https://www.ncbi.nlm.nih.gov/pubmed/33311561
http://dx.doi.org/10.1038/s41598-020-78153-0
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