Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs

BACKGROUND: There are challenges in generating mRNA-Seq data from whole-blood derived RNA as globin gene and rRNA are frequent contaminants. Given the abundance of erythrocytes in whole blood, globin genes comprise some 80% or more of the total RNA. Therefore, depletion of globin gene RNA and rRNA a...

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Detalles Bibliográficos
Autores principales: Jang, Jin Sung, Berg, Brianna, Holicky, Eileen, Eckloff, Bruce, Mutawe, Mark, Carrasquillo, Minerva M., Ertekin-Taner, Nilüfer, Cuninngham, Julie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733259/
https://www.ncbi.nlm.nih.gov/pubmed/33308163
http://dx.doi.org/10.1186/s12864-020-07304-4

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