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Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles
PURPOSE: Aseptic implant loosening is still a feared complication in the field of orthopaedics. Presumably, a chronic inflammatory response is induced by wear particles, which leads to osteoclast generation, bone degradation and hence loosening of the implant. Since it has been demonstrated in the l...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733385/ https://www.ncbi.nlm.nih.gov/pubmed/33324065 http://dx.doi.org/10.2147/TCRM.S280042 |
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author | Dapunt, Ulrike Prior, Birgit Kretzer, Jan Philippe Giese, Thomas Zhao, Yina |
author_facet | Dapunt, Ulrike Prior, Birgit Kretzer, Jan Philippe Giese, Thomas Zhao, Yina |
author_sort | Dapunt, Ulrike |
collection | PubMed |
description | PURPOSE: Aseptic implant loosening is still a feared complication in the field of orthopaedics. Presumably, a chronic inflammatory response is induced by wear particles, which leads to osteoclast generation, bone degradation and hence loosening of the implant. Since it has been demonstrated in the literature that most implants are in fact colonized by bacteria, the question arises whether aseptic implant loosening is truly aseptic. The aim of this study was to investigate a possibly enhanced inflammatory response to metal wear particles in the context of subclinical infection. PATIENTS AND METHODS: Tissue samples were collected intra-operatively from patients undergoing implant-exchange surgery due to aseptic loosening. Histopathological analysis was performed, as well as gene expression analysis for the pro-inflammatory cytokine Interleukin-8. By a series of in vitro experiments, the effect of metal wear particles on human monocytes, polymorphonuclear neutrophiles and osteoblasts was investigated. Additionally, minor amounts of lipoteichoic acid (LTA) and the bacterial heat shock protein GroEL were added. RESULTS: Histopathology of tissue samples revealed an accumulation of metal wear particles, as well as a cellular infiltrate consisting predominately of mononuclear cells. Furthermore, high expression of IL-8 could be detected in tissue surrounding the implant. Monocytes and osteoblasts in particular showed an increased release of IL-8 after stimulation with metal wear particles and in particular after stimulation with bacterial components and wear particles together. CONCLUSION: We were able to show that minor amounts of bacterial components and metal wear particles together induce an enhanced inflammatory response in human monocytes and osteoblasts. This effect could significantly contribute to the generation of bone-resorbing osteoclasts and hence implant-loosening. |
format | Online Article Text |
id | pubmed-7733385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-77333852020-12-14 Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles Dapunt, Ulrike Prior, Birgit Kretzer, Jan Philippe Giese, Thomas Zhao, Yina Ther Clin Risk Manag Original Research PURPOSE: Aseptic implant loosening is still a feared complication in the field of orthopaedics. Presumably, a chronic inflammatory response is induced by wear particles, which leads to osteoclast generation, bone degradation and hence loosening of the implant. Since it has been demonstrated in the literature that most implants are in fact colonized by bacteria, the question arises whether aseptic implant loosening is truly aseptic. The aim of this study was to investigate a possibly enhanced inflammatory response to metal wear particles in the context of subclinical infection. PATIENTS AND METHODS: Tissue samples were collected intra-operatively from patients undergoing implant-exchange surgery due to aseptic loosening. Histopathological analysis was performed, as well as gene expression analysis for the pro-inflammatory cytokine Interleukin-8. By a series of in vitro experiments, the effect of metal wear particles on human monocytes, polymorphonuclear neutrophiles and osteoblasts was investigated. Additionally, minor amounts of lipoteichoic acid (LTA) and the bacterial heat shock protein GroEL were added. RESULTS: Histopathology of tissue samples revealed an accumulation of metal wear particles, as well as a cellular infiltrate consisting predominately of mononuclear cells. Furthermore, high expression of IL-8 could be detected in tissue surrounding the implant. Monocytes and osteoblasts in particular showed an increased release of IL-8 after stimulation with metal wear particles and in particular after stimulation with bacterial components and wear particles together. CONCLUSION: We were able to show that minor amounts of bacterial components and metal wear particles together induce an enhanced inflammatory response in human monocytes and osteoblasts. This effect could significantly contribute to the generation of bone-resorbing osteoclasts and hence implant-loosening. Dove 2020-12-08 /pmc/articles/PMC7733385/ /pubmed/33324065 http://dx.doi.org/10.2147/TCRM.S280042 Text en © 2020 Dapunt et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Dapunt, Ulrike Prior, Birgit Kretzer, Jan Philippe Giese, Thomas Zhao, Yina Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles |
title | Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles |
title_full | Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles |
title_fullStr | Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles |
title_full_unstemmed | Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles |
title_short | Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles |
title_sort | bacterial biofilm components induce an enhanced inflammatory response against metal wear particles |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733385/ https://www.ncbi.nlm.nih.gov/pubmed/33324065 http://dx.doi.org/10.2147/TCRM.S280042 |
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