Cargando…

A partially self-regenerating synthetic cell

Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to regenerate essential protein components from...

Descripción completa

Detalles Bibliográficos
Autores principales: Lavickova, Barbora, Laohakunakorn, Nadanai, Maerkl, Sebastian J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733450/
https://www.ncbi.nlm.nih.gov/pubmed/33311509
http://dx.doi.org/10.1038/s41467-020-20180-6
_version_ 1783622274024210432
author Lavickova, Barbora
Laohakunakorn, Nadanai
Maerkl, Sebastian J.
author_facet Lavickova, Barbora
Laohakunakorn, Nadanai
Maerkl, Sebastian J.
author_sort Lavickova, Barbora
collection PubMed
description Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to regenerate essential protein components from DNA templates and sustain synthesis activity for over a day. By quantitating genotype-phenotype relationships combined with computational modeling we find that minimizing resource competition and optimizing resource allocation are both critically important for achieving robust system function. With this understanding, we achieve simultaneous regeneration of multiple proteins by determining the required DNA ratios necessary for sustained self-regeneration. This work introduces a conceptual and experimental framework for the development of a self-replicating synthetic cell.
format Online
Article
Text
id pubmed-7733450
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-77334502020-12-17 A partially self-regenerating synthetic cell Lavickova, Barbora Laohakunakorn, Nadanai Maerkl, Sebastian J. Nat Commun Article Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to regenerate essential protein components from DNA templates and sustain synthesis activity for over a day. By quantitating genotype-phenotype relationships combined with computational modeling we find that minimizing resource competition and optimizing resource allocation are both critically important for achieving robust system function. With this understanding, we achieve simultaneous regeneration of multiple proteins by determining the required DNA ratios necessary for sustained self-regeneration. This work introduces a conceptual and experimental framework for the development of a self-replicating synthetic cell. Nature Publishing Group UK 2020-12-11 /pmc/articles/PMC7733450/ /pubmed/33311509 http://dx.doi.org/10.1038/s41467-020-20180-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lavickova, Barbora
Laohakunakorn, Nadanai
Maerkl, Sebastian J.
A partially self-regenerating synthetic cell
title A partially self-regenerating synthetic cell
title_full A partially self-regenerating synthetic cell
title_fullStr A partially self-regenerating synthetic cell
title_full_unstemmed A partially self-regenerating synthetic cell
title_short A partially self-regenerating synthetic cell
title_sort partially self-regenerating synthetic cell
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733450/
https://www.ncbi.nlm.nih.gov/pubmed/33311509
http://dx.doi.org/10.1038/s41467-020-20180-6
work_keys_str_mv AT lavickovabarbora apartiallyselfregeneratingsyntheticcell
AT laohakunakornnadanai apartiallyselfregeneratingsyntheticcell
AT maerklsebastianj apartiallyselfregeneratingsyntheticcell
AT lavickovabarbora partiallyselfregeneratingsyntheticcell
AT laohakunakornnadanai partiallyselfregeneratingsyntheticcell
AT maerklsebastianj partiallyselfregeneratingsyntheticcell