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A partially self-regenerating synthetic cell
Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to regenerate essential protein components from...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733450/ https://www.ncbi.nlm.nih.gov/pubmed/33311509 http://dx.doi.org/10.1038/s41467-020-20180-6 |
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author | Lavickova, Barbora Laohakunakorn, Nadanai Maerkl, Sebastian J. |
author_facet | Lavickova, Barbora Laohakunakorn, Nadanai Maerkl, Sebastian J. |
author_sort | Lavickova, Barbora |
collection | PubMed |
description | Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to regenerate essential protein components from DNA templates and sustain synthesis activity for over a day. By quantitating genotype-phenotype relationships combined with computational modeling we find that minimizing resource competition and optimizing resource allocation are both critically important for achieving robust system function. With this understanding, we achieve simultaneous regeneration of multiple proteins by determining the required DNA ratios necessary for sustained self-regeneration. This work introduces a conceptual and experimental framework for the development of a self-replicating synthetic cell. |
format | Online Article Text |
id | pubmed-7733450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77334502020-12-17 A partially self-regenerating synthetic cell Lavickova, Barbora Laohakunakorn, Nadanai Maerkl, Sebastian J. Nat Commun Article Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to regenerate essential protein components from DNA templates and sustain synthesis activity for over a day. By quantitating genotype-phenotype relationships combined with computational modeling we find that minimizing resource competition and optimizing resource allocation are both critically important for achieving robust system function. With this understanding, we achieve simultaneous regeneration of multiple proteins by determining the required DNA ratios necessary for sustained self-regeneration. This work introduces a conceptual and experimental framework for the development of a self-replicating synthetic cell. Nature Publishing Group UK 2020-12-11 /pmc/articles/PMC7733450/ /pubmed/33311509 http://dx.doi.org/10.1038/s41467-020-20180-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lavickova, Barbora Laohakunakorn, Nadanai Maerkl, Sebastian J. A partially self-regenerating synthetic cell |
title | A partially self-regenerating synthetic cell |
title_full | A partially self-regenerating synthetic cell |
title_fullStr | A partially self-regenerating synthetic cell |
title_full_unstemmed | A partially self-regenerating synthetic cell |
title_short | A partially self-regenerating synthetic cell |
title_sort | partially self-regenerating synthetic cell |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733450/ https://www.ncbi.nlm.nih.gov/pubmed/33311509 http://dx.doi.org/10.1038/s41467-020-20180-6 |
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