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Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats
Mechanisms involved in the acute responses to renal denervation (RDN) have yet to be fully understood. We assessed urinary volume, autonomic control and aorta vascular reactivity after acute RDN. Male normotensive Wistar rats and spontaneously hypertensive rats (SHR) were divided into normotensive +...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733454/ https://www.ncbi.nlm.nih.gov/pubmed/33311525 http://dx.doi.org/10.1038/s41598-020-78674-8 |
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author | Moreira, Nathalia Juocys Dias dos Santos, Fernando Moreira, Edson Dias Farah, Daniela de Souza, Leandro Eziquiel da Silva, Maikon Barbosa Moraes-Silva, Ivana Cinthya Lincevicius, Gisele Silvério Caldini, Elia Garcia Irigoyen, Maria Cláudia Costa |
author_facet | Moreira, Nathalia Juocys Dias dos Santos, Fernando Moreira, Edson Dias Farah, Daniela de Souza, Leandro Eziquiel da Silva, Maikon Barbosa Moraes-Silva, Ivana Cinthya Lincevicius, Gisele Silvério Caldini, Elia Garcia Irigoyen, Maria Cláudia Costa |
author_sort | Moreira, Nathalia Juocys Dias |
collection | PubMed |
description | Mechanisms involved in the acute responses to renal denervation (RDN) have yet to be fully understood. We assessed urinary volume, autonomic control and aorta vascular reactivity after acute RDN. Male normotensive Wistar rats and spontaneously hypertensive rats (SHR) were divided into normotensive + RDN (ND) or sham surgery (NS), and hypertensive + RDN (HD) or sham surgery (HS). Metabolic parameters and hemodynamic measurements were recorded 72h and 4 days after intervention, respectively. Aortic rings were studied 7 days post RDN in an isometric myograph. Concentration–response curves to phenylephrine, sodium nitroprusside and acetylcholine (10(–10)–10(−5) M) were performed. Two-way ANOVA was used for group comparisons and differences reported when p < 0.05. Results are presented as mean ± SEM. Urinary volume was 112% higher in HD vs. HS (HS = 14.94 ± 2.5 mL; HD = 31.69 ± 2.2 mL) and remained unchanged in normotensive rats. Systolic BP was lower in HD rats (HS = 201 ± 12 vs. HD = 172 ± 3 mmHg) without changes in normotensive group. HD group showed increased HF and LF modulation (HS = 5.8 ± 0.7 ms(2) vs. HD = 13.4 ± 1.4 ms(2); HS = 3.5 ± 0.7 ms(2) vs. HD = 10.5 ± 1.7 ms(2), respectively). RDN normalized vascular reactivity in HD rats and increased phenylephrine response in ND rats. Acute fall in BP induced by RDN is associated with increased urinary volume, which in turn may also have contributed to functional changes of the aorta. |
format | Online Article Text |
id | pubmed-7733454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77334542020-12-15 Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats Moreira, Nathalia Juocys Dias dos Santos, Fernando Moreira, Edson Dias Farah, Daniela de Souza, Leandro Eziquiel da Silva, Maikon Barbosa Moraes-Silva, Ivana Cinthya Lincevicius, Gisele Silvério Caldini, Elia Garcia Irigoyen, Maria Cláudia Costa Sci Rep Article Mechanisms involved in the acute responses to renal denervation (RDN) have yet to be fully understood. We assessed urinary volume, autonomic control and aorta vascular reactivity after acute RDN. Male normotensive Wistar rats and spontaneously hypertensive rats (SHR) were divided into normotensive + RDN (ND) or sham surgery (NS), and hypertensive + RDN (HD) or sham surgery (HS). Metabolic parameters and hemodynamic measurements were recorded 72h and 4 days after intervention, respectively. Aortic rings were studied 7 days post RDN in an isometric myograph. Concentration–response curves to phenylephrine, sodium nitroprusside and acetylcholine (10(–10)–10(−5) M) were performed. Two-way ANOVA was used for group comparisons and differences reported when p < 0.05. Results are presented as mean ± SEM. Urinary volume was 112% higher in HD vs. HS (HS = 14.94 ± 2.5 mL; HD = 31.69 ± 2.2 mL) and remained unchanged in normotensive rats. Systolic BP was lower in HD rats (HS = 201 ± 12 vs. HD = 172 ± 3 mmHg) without changes in normotensive group. HD group showed increased HF and LF modulation (HS = 5.8 ± 0.7 ms(2) vs. HD = 13.4 ± 1.4 ms(2); HS = 3.5 ± 0.7 ms(2) vs. HD = 10.5 ± 1.7 ms(2), respectively). RDN normalized vascular reactivity in HD rats and increased phenylephrine response in ND rats. Acute fall in BP induced by RDN is associated with increased urinary volume, which in turn may also have contributed to functional changes of the aorta. Nature Publishing Group UK 2020-12-11 /pmc/articles/PMC7733454/ /pubmed/33311525 http://dx.doi.org/10.1038/s41598-020-78674-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Moreira, Nathalia Juocys Dias dos Santos, Fernando Moreira, Edson Dias Farah, Daniela de Souza, Leandro Eziquiel da Silva, Maikon Barbosa Moraes-Silva, Ivana Cinthya Lincevicius, Gisele Silvério Caldini, Elia Garcia Irigoyen, Maria Cláudia Costa Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats |
title | Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats |
title_full | Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats |
title_fullStr | Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats |
title_full_unstemmed | Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats |
title_short | Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats |
title_sort | acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733454/ https://www.ncbi.nlm.nih.gov/pubmed/33311525 http://dx.doi.org/10.1038/s41598-020-78674-8 |
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