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Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis

Chronic periodontitis (CP) is an oral cavity disease arising from chronic inflammation of the periodontal tissues. Exosomes are lipid vesicles that are enriched in specific microRNAs (miRNAs), potentially providing a disease-specific diagnostic signature. To assess the value of exosomal miRNAs as bi...

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Autores principales: Nik Mohamed Kamal, Nik Nur Syazana, Awang, Raja Azman Raja, Mohamad, Suharni, Shahidan, Wan Nazatul Shima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733931/
https://www.ncbi.nlm.nih.gov/pubmed/33329037
http://dx.doi.org/10.3389/fphys.2020.587381
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author Nik Mohamed Kamal, Nik Nur Syazana
Awang, Raja Azman Raja
Mohamad, Suharni
Shahidan, Wan Nazatul Shima
author_facet Nik Mohamed Kamal, Nik Nur Syazana
Awang, Raja Azman Raja
Mohamad, Suharni
Shahidan, Wan Nazatul Shima
author_sort Nik Mohamed Kamal, Nik Nur Syazana
collection PubMed
description Chronic periodontitis (CP) is an oral cavity disease arising from chronic inflammation of the periodontal tissues. Exosomes are lipid vesicles that are enriched in specific microRNAs (miRNAs), potentially providing a disease-specific diagnostic signature. To assess the value of exosomal miRNAs as biomarkers for CP, 8 plasma- and 8 salivary-exosomal miRNAs samples were profiled using Agilent platform (comparative study). From 2,549 probed miRNAs, 33 miRNAs were significantly down-regulated in CP as compared to healthy plasma samples. Whereas, 1,995 miRNAs (1,985 down-regulated and 10 up-regulated) were differentially expressed in the CP as compared to healthy saliva samples. hsa-miR-let-7d [FC = −26.76; AUC = 1; r = −0.728 [p-value = 0.04]), hsa-miR-126-3p (FC = −24.02; AUC = 1; r = −0.723 [p-value = 0.043]) and hsa-miR-199a-3p (FC = −22.94; AUC = 1; r = −0.731 [p-value = 0.039]) are worth to be furthered studied for plasma-exosomal samples. Meanwhile, for salivary-exosomal samples, hsa-miR-125a-3p (FC = 2.03; AUC = 1; r = 0.91 [p-value = 0.02]) is worth to be furthered studied. These miRNAs are the reliable candidates for the development of periodontitis biomarker, as they were significantly expressed differently between CP and healthy samples, have a good discriminatory value and strongly correlate with the mean of PPD. These findings highlight the potential of exosomal miRNAs profiling in the diagnosis from both sourced as well as provide new insights into the molecular mechanisms involved in CP.
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spelling pubmed-77339312020-12-15 Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis Nik Mohamed Kamal, Nik Nur Syazana Awang, Raja Azman Raja Mohamad, Suharni Shahidan, Wan Nazatul Shima Front Physiol Physiology Chronic periodontitis (CP) is an oral cavity disease arising from chronic inflammation of the periodontal tissues. Exosomes are lipid vesicles that are enriched in specific microRNAs (miRNAs), potentially providing a disease-specific diagnostic signature. To assess the value of exosomal miRNAs as biomarkers for CP, 8 plasma- and 8 salivary-exosomal miRNAs samples were profiled using Agilent platform (comparative study). From 2,549 probed miRNAs, 33 miRNAs were significantly down-regulated in CP as compared to healthy plasma samples. Whereas, 1,995 miRNAs (1,985 down-regulated and 10 up-regulated) were differentially expressed in the CP as compared to healthy saliva samples. hsa-miR-let-7d [FC = −26.76; AUC = 1; r = −0.728 [p-value = 0.04]), hsa-miR-126-3p (FC = −24.02; AUC = 1; r = −0.723 [p-value = 0.043]) and hsa-miR-199a-3p (FC = −22.94; AUC = 1; r = −0.731 [p-value = 0.039]) are worth to be furthered studied for plasma-exosomal samples. Meanwhile, for salivary-exosomal samples, hsa-miR-125a-3p (FC = 2.03; AUC = 1; r = 0.91 [p-value = 0.02]) is worth to be furthered studied. These miRNAs are the reliable candidates for the development of periodontitis biomarker, as they were significantly expressed differently between CP and healthy samples, have a good discriminatory value and strongly correlate with the mean of PPD. These findings highlight the potential of exosomal miRNAs profiling in the diagnosis from both sourced as well as provide new insights into the molecular mechanisms involved in CP. Frontiers Media S.A. 2020-11-30 /pmc/articles/PMC7733931/ /pubmed/33329037 http://dx.doi.org/10.3389/fphys.2020.587381 Text en Copyright © 2020 Nik Mohamed Kamal, Awang, Mohamad and Shahidan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Nik Mohamed Kamal, Nik Nur Syazana
Awang, Raja Azman Raja
Mohamad, Suharni
Shahidan, Wan Nazatul Shima
Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis
title Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis
title_full Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis
title_fullStr Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis
title_full_unstemmed Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis
title_short Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis
title_sort plasma- and saliva exosome profile reveals a distinct microrna signature in chronic periodontitis
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733931/
https://www.ncbi.nlm.nih.gov/pubmed/33329037
http://dx.doi.org/10.3389/fphys.2020.587381
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