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Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: randomized control trial in Singapore

PURPOSE: One of the key approaches to minimize the risk of COVID-19 transmission would be to reduce the titres of SARS-CoV-2 in the saliva of infected COVID-19 patients. This is particularly important in high-risk procedures like dental treatment. The present randomized control trial evaluated the e...

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Autores principales: Seneviratne, Chaminda J., Balan, Preethi, Ko, Kwan Ki Karrie, Udawatte, Nadeeka S., Lai, Deborah, Ng, Dorothy Hui Lin, Venkatachalam, Indumathi, Lim, Kheng Sit, Ling, Moi Lin, Oon, Lynette, Goh, Bee Tin, Sim, Xiang Ying Jean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734110/
https://www.ncbi.nlm.nih.gov/pubmed/33315181
http://dx.doi.org/10.1007/s15010-020-01563-9
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author Seneviratne, Chaminda J.
Balan, Preethi
Ko, Kwan Ki Karrie
Udawatte, Nadeeka S.
Lai, Deborah
Ng, Dorothy Hui Lin
Venkatachalam, Indumathi
Lim, Kheng Sit
Ling, Moi Lin
Oon, Lynette
Goh, Bee Tin
Sim, Xiang Ying Jean
author_facet Seneviratne, Chaminda J.
Balan, Preethi
Ko, Kwan Ki Karrie
Udawatte, Nadeeka S.
Lai, Deborah
Ng, Dorothy Hui Lin
Venkatachalam, Indumathi
Lim, Kheng Sit
Ling, Moi Lin
Oon, Lynette
Goh, Bee Tin
Sim, Xiang Ying Jean
author_sort Seneviratne, Chaminda J.
collection PubMed
description PURPOSE: One of the key approaches to minimize the risk of COVID-19 transmission would be to reduce the titres of SARS-CoV-2 in the saliva of infected COVID-19 patients. This is particularly important in high-risk procedures like dental treatment. The present randomized control trial evaluated the efficacy of three commercial mouth-rinse viz. povidone–iodine (PI), chlorhexidine gluconate (CHX) and cetylpyridinium chloride (CPC), in reducing the salivary SARS-CoV-2 viral load in COVID-19 patients compared with water. METHODS: A total of 36 SARS-CoV-2-positive patients were recruited, of which 16 patients were randomly assigned to four groups—PI group (n = 4), CHX group (n = 6), CPC group (n = 4) and water as control group (n = 2). Saliva samples were collected from all patients at baseline and at 5 min, 3 h and 6 h post-application of mouth-rinses/water. The samples were subjected to SARS-CoV-2 RT-PCR analysis. RESULTS: Comparison of salivary Ct values of patients within each group of PI, CHX, CPC and water at 5 min, 3 h and 6 h time points did not show any significant differences. However, when the Ct value fold change of each of the mouth-rinse group patients were compared with the fold change of water group patients at the respective time points, a significant increase was observed in the CPC group patients at 5 min and 6 h and in the PI group patients at 6 h. CONCLUSION: The effect of decreasing salivary load with CPC and PI mouth-rinsing was observed to be sustained at 6 h time point. Within the limitation of the current study, as number of the samples analyzed, the use of CPC and PI formulated that commercial mouth-rinses may be useful as a pre-procedural rinse to help reduce the transmission of COVID-19. ISRCTN (ISRCTN95933274), 09/09/20, retrospectively registered
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spelling pubmed-77341102020-12-14 Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: randomized control trial in Singapore Seneviratne, Chaminda J. Balan, Preethi Ko, Kwan Ki Karrie Udawatte, Nadeeka S. Lai, Deborah Ng, Dorothy Hui Lin Venkatachalam, Indumathi Lim, Kheng Sit Ling, Moi Lin Oon, Lynette Goh, Bee Tin Sim, Xiang Ying Jean Infection Original Paper PURPOSE: One of the key approaches to minimize the risk of COVID-19 transmission would be to reduce the titres of SARS-CoV-2 in the saliva of infected COVID-19 patients. This is particularly important in high-risk procedures like dental treatment. The present randomized control trial evaluated the efficacy of three commercial mouth-rinse viz. povidone–iodine (PI), chlorhexidine gluconate (CHX) and cetylpyridinium chloride (CPC), in reducing the salivary SARS-CoV-2 viral load in COVID-19 patients compared with water. METHODS: A total of 36 SARS-CoV-2-positive patients were recruited, of which 16 patients were randomly assigned to four groups—PI group (n = 4), CHX group (n = 6), CPC group (n = 4) and water as control group (n = 2). Saliva samples were collected from all patients at baseline and at 5 min, 3 h and 6 h post-application of mouth-rinses/water. The samples were subjected to SARS-CoV-2 RT-PCR analysis. RESULTS: Comparison of salivary Ct values of patients within each group of PI, CHX, CPC and water at 5 min, 3 h and 6 h time points did not show any significant differences. However, when the Ct value fold change of each of the mouth-rinse group patients were compared with the fold change of water group patients at the respective time points, a significant increase was observed in the CPC group patients at 5 min and 6 h and in the PI group patients at 6 h. CONCLUSION: The effect of decreasing salivary load with CPC and PI mouth-rinsing was observed to be sustained at 6 h time point. Within the limitation of the current study, as number of the samples analyzed, the use of CPC and PI formulated that commercial mouth-rinses may be useful as a pre-procedural rinse to help reduce the transmission of COVID-19. ISRCTN (ISRCTN95933274), 09/09/20, retrospectively registered Springer Berlin Heidelberg 2020-12-14 2021 /pmc/articles/PMC7734110/ /pubmed/33315181 http://dx.doi.org/10.1007/s15010-020-01563-9 Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Paper
Seneviratne, Chaminda J.
Balan, Preethi
Ko, Kwan Ki Karrie
Udawatte, Nadeeka S.
Lai, Deborah
Ng, Dorothy Hui Lin
Venkatachalam, Indumathi
Lim, Kheng Sit
Ling, Moi Lin
Oon, Lynette
Goh, Bee Tin
Sim, Xiang Ying Jean
Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: randomized control trial in Singapore
title Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: randomized control trial in Singapore
title_full Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: randomized control trial in Singapore
title_fullStr Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: randomized control trial in Singapore
title_full_unstemmed Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: randomized control trial in Singapore
title_short Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: randomized control trial in Singapore
title_sort efficacy of commercial mouth-rinses on sars-cov-2 viral load in saliva: randomized control trial in singapore
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734110/
https://www.ncbi.nlm.nih.gov/pubmed/33315181
http://dx.doi.org/10.1007/s15010-020-01563-9
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