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Metabolic impact of persistent organic pollutants on gut microbiota

Emerging evidence supports that exposure to persistent organic pollutants (POPs) can impact the interaction between the gut microbiota and host. Recent efforts have characterized the relationship between gut microbiota and environment pollutants suggesting additional research is needed to understand...

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Autores principales: Tian, Yuan, Gui, Wei, Rimal, Bipin, Koo, Imhoi, Smith, Philip B., Nichols, Robert G., Cai, Jingwei, Liu, Qing, Patterson, Andrew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734116/
https://www.ncbi.nlm.nih.gov/pubmed/33295235
http://dx.doi.org/10.1080/19490976.2020.1848209
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author Tian, Yuan
Gui, Wei
Rimal, Bipin
Koo, Imhoi
Smith, Philip B.
Nichols, Robert G.
Cai, Jingwei
Liu, Qing
Patterson, Andrew D.
author_facet Tian, Yuan
Gui, Wei
Rimal, Bipin
Koo, Imhoi
Smith, Philip B.
Nichols, Robert G.
Cai, Jingwei
Liu, Qing
Patterson, Andrew D.
author_sort Tian, Yuan
collection PubMed
description Emerging evidence supports that exposure to persistent organic pollutants (POPs) can impact the interaction between the gut microbiota and host. Recent efforts have characterized the relationship between gut microbiota and environment pollutants suggesting additional research is needed to understand potential new avenues for toxicity. Here, we systematically examined the direct effects of POPs including 2,3,7,8-tetrachlorodibenzofuran (TCDF), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and polychlorinated biphenyls (PCB-123 and PCB-156) on the microbiota using metatranscriptomics and NMR- and mass spectrometry-based metabolomics combined with flow cytometry and growth rate measurements (OD(600)). This study demonstrated that (1) POPs directly and rapidly affect isolated cecal bacterial global metabolism that is associated with significant decreases in microbial metabolic activity; (2) significant changes in cecal bacterial gene expression related to tricarboxylic acid (TCA) cycle as well as carbon metabolism, carbon fixation, pyruvate metabolism, and protein export were observed following most POP exposure; (3) six individual bacterial species show variation in lipid metabolism in response to POP exposure; and (4) PCB-153 (non-coplanar)has a greater impact on bacteria than PCB-126 (coplanar) at the metabolic and transcriptional levels. These data provide new insights into the direct role of POPs on gut microbiota and begins to establish possible microbial toxicity endpoints which may help to inform risk assessment.
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spelling pubmed-77341162020-12-18 Metabolic impact of persistent organic pollutants on gut microbiota Tian, Yuan Gui, Wei Rimal, Bipin Koo, Imhoi Smith, Philip B. Nichols, Robert G. Cai, Jingwei Liu, Qing Patterson, Andrew D. Gut Microbes Research Paper Emerging evidence supports that exposure to persistent organic pollutants (POPs) can impact the interaction between the gut microbiota and host. Recent efforts have characterized the relationship between gut microbiota and environment pollutants suggesting additional research is needed to understand potential new avenues for toxicity. Here, we systematically examined the direct effects of POPs including 2,3,7,8-tetrachlorodibenzofuran (TCDF), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and polychlorinated biphenyls (PCB-123 and PCB-156) on the microbiota using metatranscriptomics and NMR- and mass spectrometry-based metabolomics combined with flow cytometry and growth rate measurements (OD(600)). This study demonstrated that (1) POPs directly and rapidly affect isolated cecal bacterial global metabolism that is associated with significant decreases in microbial metabolic activity; (2) significant changes in cecal bacterial gene expression related to tricarboxylic acid (TCA) cycle as well as carbon metabolism, carbon fixation, pyruvate metabolism, and protein export were observed following most POP exposure; (3) six individual bacterial species show variation in lipid metabolism in response to POP exposure; and (4) PCB-153 (non-coplanar)has a greater impact on bacteria than PCB-126 (coplanar) at the metabolic and transcriptional levels. These data provide new insights into the direct role of POPs on gut microbiota and begins to establish possible microbial toxicity endpoints which may help to inform risk assessment. Taylor & Francis 2020-12-09 /pmc/articles/PMC7734116/ /pubmed/33295235 http://dx.doi.org/10.1080/19490976.2020.1848209 Text en © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Tian, Yuan
Gui, Wei
Rimal, Bipin
Koo, Imhoi
Smith, Philip B.
Nichols, Robert G.
Cai, Jingwei
Liu, Qing
Patterson, Andrew D.
Metabolic impact of persistent organic pollutants on gut microbiota
title Metabolic impact of persistent organic pollutants on gut microbiota
title_full Metabolic impact of persistent organic pollutants on gut microbiota
title_fullStr Metabolic impact of persistent organic pollutants on gut microbiota
title_full_unstemmed Metabolic impact of persistent organic pollutants on gut microbiota
title_short Metabolic impact of persistent organic pollutants on gut microbiota
title_sort metabolic impact of persistent organic pollutants on gut microbiota
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734116/
https://www.ncbi.nlm.nih.gov/pubmed/33295235
http://dx.doi.org/10.1080/19490976.2020.1848209
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