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Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia

Citrus leaf and fruit spot is one of the most important biotic constraints of citrus production in Ethiopia. The symptomatic leaf and fruit samples were collected from 29 orchards of 15 major citrus growing districts of Ethiopia. One hundred sixty-seven fungal isolates were recovered and identified...

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Autores principales: AD, Moges, D, Belew, B, Admassu, M, Yesuf, S, Maina, SR, Ghimire
Formato: Online Artículo Texto
Lenguaje:English
Publicado: OMICS Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734371/
https://www.ncbi.nlm.nih.gov/pubmed/33384880
http://dx.doi.org/10.4172/2157-7471.1000425
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author AD, Moges
D, Belew
B, Admassu
M, Yesuf
S, Maina
SR, Ghimire
author_facet AD, Moges
D, Belew
B, Admassu
M, Yesuf
S, Maina
SR, Ghimire
author_sort AD, Moges
collection PubMed
description Citrus leaf and fruit spot is one of the most important biotic constraints of citrus production in Ethiopia. The symptomatic leaf and fruit samples were collected from 29 orchards of 15 major citrus growing districts of Ethiopia. One hundred sixty-seven fungal isolates were recovered and identified to species level through DNA barcoding; and their relationships were established using multigene phylogeny. The internal transcribed spacers, long subunit and actin gene sequences revealed that those 167 isolates belonged to either Collectotrichum gloeosporioides or Collectotrichum boninense species complexes (sensu lato), but no recovery of Pseudocercospora angolensis, the primary causal agent of the citrus leaf and fruit spot disease. Detached leaf assays confirmed pathogenicity of isolates of both C. gloeosporioides and C. boninense species complexes on citrus. They reproduced disease symptoms and the pathogens were re-isolated from symptomatic tissues. This study reports frequent association of C. gloeosporioides and C. boninense species complexes with citrus fruit and leaf spot disease in Ethiopia. This finding suggests the need for in-depth studies to determine the roles of C. gloeosporioides and C. boninense species complexes in citrus fruit and leaf spot disease epidemiology.
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spelling pubmed-77343712020-12-29 Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia AD, Moges D, Belew B, Admassu M, Yesuf S, Maina SR, Ghimire J Plant Pathol Microbiol Research Article Citrus leaf and fruit spot is one of the most important biotic constraints of citrus production in Ethiopia. The symptomatic leaf and fruit samples were collected from 29 orchards of 15 major citrus growing districts of Ethiopia. One hundred sixty-seven fungal isolates were recovered and identified to species level through DNA barcoding; and their relationships were established using multigene phylogeny. The internal transcribed spacers, long subunit and actin gene sequences revealed that those 167 isolates belonged to either Collectotrichum gloeosporioides or Collectotrichum boninense species complexes (sensu lato), but no recovery of Pseudocercospora angolensis, the primary causal agent of the citrus leaf and fruit spot disease. Detached leaf assays confirmed pathogenicity of isolates of both C. gloeosporioides and C. boninense species complexes on citrus. They reproduced disease symptoms and the pathogens were re-isolated from symptomatic tissues. This study reports frequent association of C. gloeosporioides and C. boninense species complexes with citrus fruit and leaf spot disease in Ethiopia. This finding suggests the need for in-depth studies to determine the roles of C. gloeosporioides and C. boninense species complexes in citrus fruit and leaf spot disease epidemiology. OMICS Publishing Group 2017-10-26 2017 /pmc/articles/PMC7734371/ /pubmed/33384880 http://dx.doi.org/10.4172/2157-7471.1000425 Text en © 2017 Moges AD http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
AD, Moges
D, Belew
B, Admassu
M, Yesuf
S, Maina
SR, Ghimire
Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia
title Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia
title_full Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia
title_fullStr Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia
title_full_unstemmed Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia
title_short Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia
title_sort frequent association of colletotrichum species with citrus fruit and leaf spot disease symptoms and their genetic diversity in ethiopia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734371/
https://www.ncbi.nlm.nih.gov/pubmed/33384880
http://dx.doi.org/10.4172/2157-7471.1000425
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