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Electropermeabilization of nematode eggs for parasite deactivation

The eggs of parasitic helminth worms are incredibly resilient - possessing the ability to survive changing environmental factors and exposure to chemical treatments - which has restricted the efficacy of wastewater sanitation. This research reports on the effectiveness of electroporation to permeabi...

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Autores principales: Dryzer, M. H., Niven, C., Wolter, S. D., Arena, C. B., Ngaboyamahina, E., Parker, C. B., Stoner, B. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IWA Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734379/
https://www.ncbi.nlm.nih.gov/pubmed/33384870
http://dx.doi.org/10.2166/washdev.2019.100
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author Dryzer, M. H.
Niven, C.
Wolter, S. D.
Arena, C. B.
Ngaboyamahina, E.
Parker, C. B.
Stoner, B. R.
author_facet Dryzer, M. H.
Niven, C.
Wolter, S. D.
Arena, C. B.
Ngaboyamahina, E.
Parker, C. B.
Stoner, B. R.
author_sort Dryzer, M. H.
collection PubMed
description The eggs of parasitic helminth worms are incredibly resilient - possessing the ability to survive changing environmental factors and exposure to chemical treatments - which has restricted the efficacy of wastewater sanitation. This research reports on the effectiveness of electroporation to permeabilize ova of Caenorhabditis elegans (C. elegans), a helminth surrogate, for parasite deactivation. This technique utilizes electric pulses to increase cell membrane permeability in its conventional application, but herein is used to open pores in nonparasitic nematode eggshells - the first report of such an application to the best knowledge of the authors. A parametric evaluation of electric field strength and total electroporation duration of eggs and worms in phosphate-buffered saline was performed using a 1 Hz pulse train of 0.01% duty cycle. The extent of pore formation was determined using a fluorescent label, propidium iodide, targeting C. elegans embryonic DNA. The results of this research demonstrate that electroporation increases eggshell permeability. This treatment, coupled with existing methods of electrochemical disinfection, could improve upon current attempts at the deactivation of helminth eggs. We discuss electroporation treatment conditions and likely modification of the lipid-rich permeability barrier within the eggshell strata.
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spelling pubmed-77343792020-12-29 Electropermeabilization of nematode eggs for parasite deactivation Dryzer, M. H. Niven, C. Wolter, S. D. Arena, C. B. Ngaboyamahina, E. Parker, C. B. Stoner, B. R. J Water Sanit Hyg Dev Research Article The eggs of parasitic helminth worms are incredibly resilient - possessing the ability to survive changing environmental factors and exposure to chemical treatments - which has restricted the efficacy of wastewater sanitation. This research reports on the effectiveness of electroporation to permeabilize ova of Caenorhabditis elegans (C. elegans), a helminth surrogate, for parasite deactivation. This technique utilizes electric pulses to increase cell membrane permeability in its conventional application, but herein is used to open pores in nonparasitic nematode eggshells - the first report of such an application to the best knowledge of the authors. A parametric evaluation of electric field strength and total electroporation duration of eggs and worms in phosphate-buffered saline was performed using a 1 Hz pulse train of 0.01% duty cycle. The extent of pore formation was determined using a fluorescent label, propidium iodide, targeting C. elegans embryonic DNA. The results of this research demonstrate that electroporation increases eggshell permeability. This treatment, coupled with existing methods of electrochemical disinfection, could improve upon current attempts at the deactivation of helminth eggs. We discuss electroporation treatment conditions and likely modification of the lipid-rich permeability barrier within the eggshell strata. IWA Publishing 2019-01-02 /pmc/articles/PMC7734379/ /pubmed/33384870 http://dx.doi.org/10.2166/washdev.2019.100 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY-NC-ND 4.0), which permits copying and redistribution for non-commercial purposes with no derivatives, provided the original work is properly cited (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Dryzer, M. H.
Niven, C.
Wolter, S. D.
Arena, C. B.
Ngaboyamahina, E.
Parker, C. B.
Stoner, B. R.
Electropermeabilization of nematode eggs for parasite deactivation
title Electropermeabilization of nematode eggs for parasite deactivation
title_full Electropermeabilization of nematode eggs for parasite deactivation
title_fullStr Electropermeabilization of nematode eggs for parasite deactivation
title_full_unstemmed Electropermeabilization of nematode eggs for parasite deactivation
title_short Electropermeabilization of nematode eggs for parasite deactivation
title_sort electropermeabilization of nematode eggs for parasite deactivation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734379/
https://www.ncbi.nlm.nih.gov/pubmed/33384870
http://dx.doi.org/10.2166/washdev.2019.100
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