Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius

The metabolic engineering of carbon monoxide (CO) oxidizers has the potential to create efficient biocatalysts to produce hydrogen and other valuable chemicals. We herein applied markerless gene deletion to CO dehydrogenase/energy-converting hydrogenase (CODH/ECH) in the thermophilic facultative ana...

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Autores principales: Adachi, Yuka, Inoue, Masao, Yoshida, Takashi, Sako, Yoshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734403/
https://www.ncbi.nlm.nih.gov/pubmed/33087627
http://dx.doi.org/10.1264/jsme2.ME20101
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author Adachi, Yuka
Inoue, Masao
Yoshida, Takashi
Sako, Yoshihiko
author_facet Adachi, Yuka
Inoue, Masao
Yoshida, Takashi
Sako, Yoshihiko
author_sort Adachi, Yuka
collection PubMed
description The metabolic engineering of carbon monoxide (CO) oxidizers has the potential to create efficient biocatalysts to produce hydrogen and other valuable chemicals. We herein applied markerless gene deletion to CO dehydrogenase/energy-converting hydrogenase (CODH/ECH) in the thermophilic facultative anaerobe, Parageobacillus thermoglucosidasius. We initially compared the transformation efficiency of two strains, NBRC 107763(T) and TG4. We then disrupted CODH, ECH, and both enzymes in NBRC 107763(T). The characterization of growth in all three disruptants under 100% CO demonstrated that both enzymes were essential for CO-dependent growth with hydrogen production in P. thermoglucosidasius. The present results will become a platform for the further metabolic engineering of this organism.
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spelling pubmed-77344032020-12-18 Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius Adachi, Yuka Inoue, Masao Yoshida, Takashi Sako, Yoshihiko Microbes Environ Short Communication The metabolic engineering of carbon monoxide (CO) oxidizers has the potential to create efficient biocatalysts to produce hydrogen and other valuable chemicals. We herein applied markerless gene deletion to CO dehydrogenase/energy-converting hydrogenase (CODH/ECH) in the thermophilic facultative anaerobe, Parageobacillus thermoglucosidasius. We initially compared the transformation efficiency of two strains, NBRC 107763(T) and TG4. We then disrupted CODH, ECH, and both enzymes in NBRC 107763(T). The characterization of growth in all three disruptants under 100% CO demonstrated that both enzymes were essential for CO-dependent growth with hydrogen production in P. thermoglucosidasius. The present results will become a platform for the further metabolic engineering of this organism. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2020 2020-10-22 /pmc/articles/PMC7734403/ /pubmed/33087627 http://dx.doi.org/10.1264/jsme2.ME20101 Text en 2020 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Adachi, Yuka
Inoue, Masao
Yoshida, Takashi
Sako, Yoshihiko
Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius
title Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius
title_full Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius
title_fullStr Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius
title_full_unstemmed Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius
title_short Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius
title_sort genetic engineering of carbon monoxide-dependent hydrogen-producing machinery in parageobacillus thermoglucosidasius
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734403/
https://www.ncbi.nlm.nih.gov/pubmed/33087627
http://dx.doi.org/10.1264/jsme2.ME20101
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