Cargando…
Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application
In the past thirty years, the biosafety of the aboveground part of crops, including horizontal gene transferal through pollen dispersal and hybridization, has been the focus of research; however, microbial communities in the underground part are attracting increasing attention. In the present study,...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734404/ https://www.ncbi.nlm.nih.gov/pubmed/33162465 http://dx.doi.org/10.1264/jsme2.ME20056 |
_version_ | 1783622467053420544 |
---|---|
author | Yang, Minkai Wen, Zhongling Fazal, Aliya Hua, Xiaomei Xu, Xinhong Yin, Tongming Qi, Jinliang Yang, Rongwu Lu, Guihua Hong, Zhi Yang, Yonghua |
author_facet | Yang, Minkai Wen, Zhongling Fazal, Aliya Hua, Xiaomei Xu, Xinhong Yin, Tongming Qi, Jinliang Yang, Rongwu Lu, Guihua Hong, Zhi Yang, Yonghua |
author_sort | Yang, Minkai |
collection | PubMed |
description | In the past thirty years, the biosafety of the aboveground part of crops, including horizontal gene transferal through pollen dispersal and hybridization, has been the focus of research; however, microbial communities in the underground part are attracting increasing attention. In the present study, the soybean root-associated bacterial communities of the G2-EPSPS plus GAT transgenic soybean line Z106, its recipient variety ZH10, and Z106 treated with glyphosate (Z106J) were compared at the seedling, flowering, and seed filling stages by high-throughput sequencing of the V4 hypervariable regions of 16S rRNA gene amplicons using Illumina MiSeq. The results obtained showed no significant differences in the alpha/beta diversities of root-associated bacterial communities at the three stages among ZH10, Z106, and Z106J under field growth conditions; however, the relative abundance of four main nitrogen-fixing bacterial genera significantly differed among ZH10, Z106, and Z106J. Ternary plot results indicated that in the root compartment, the proportional contributions of rhizobial nitrogen-fixing Ensifer fredii and Bradyrhizobium elkanii, which exhibit an extremely broad nodulation host range, markedly differed among the three treatments at the three stages. Thus, the present results indicate that transgenic G2-EPSPS and GAT soybean may induce different changes in functional bacterial species in soil, such as E. fredii and B. elkanii, from ZH10, which were compensated for/enriched at the flowering and seed filling stages, respectively, to some extent through as of yet unknown mechanisms by transgenic soybean treated with glyphosate. |
format | Online Article Text |
id | pubmed-7734404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles |
record_format | MEDLINE/PubMed |
spelling | pubmed-77344042020-12-18 Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application Yang, Minkai Wen, Zhongling Fazal, Aliya Hua, Xiaomei Xu, Xinhong Yin, Tongming Qi, Jinliang Yang, Rongwu Lu, Guihua Hong, Zhi Yang, Yonghua Microbes Environ Regular Paper In the past thirty years, the biosafety of the aboveground part of crops, including horizontal gene transferal through pollen dispersal and hybridization, has been the focus of research; however, microbial communities in the underground part are attracting increasing attention. In the present study, the soybean root-associated bacterial communities of the G2-EPSPS plus GAT transgenic soybean line Z106, its recipient variety ZH10, and Z106 treated with glyphosate (Z106J) were compared at the seedling, flowering, and seed filling stages by high-throughput sequencing of the V4 hypervariable regions of 16S rRNA gene amplicons using Illumina MiSeq. The results obtained showed no significant differences in the alpha/beta diversities of root-associated bacterial communities at the three stages among ZH10, Z106, and Z106J under field growth conditions; however, the relative abundance of four main nitrogen-fixing bacterial genera significantly differed among ZH10, Z106, and Z106J. Ternary plot results indicated that in the root compartment, the proportional contributions of rhizobial nitrogen-fixing Ensifer fredii and Bradyrhizobium elkanii, which exhibit an extremely broad nodulation host range, markedly differed among the three treatments at the three stages. Thus, the present results indicate that transgenic G2-EPSPS and GAT soybean may induce different changes in functional bacterial species in soil, such as E. fredii and B. elkanii, from ZH10, which were compensated for/enriched at the flowering and seed filling stages, respectively, to some extent through as of yet unknown mechanisms by transgenic soybean treated with glyphosate. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2020 2020-11-07 /pmc/articles/PMC7734404/ /pubmed/33162465 http://dx.doi.org/10.1264/jsme2.ME20056 Text en 2020 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Paper Yang, Minkai Wen, Zhongling Fazal, Aliya Hua, Xiaomei Xu, Xinhong Yin, Tongming Qi, Jinliang Yang, Rongwu Lu, Guihua Hong, Zhi Yang, Yonghua Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application |
title | Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application |
title_full | Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application |
title_fullStr | Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application |
title_full_unstemmed | Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application |
title_short | Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application |
title_sort | impact of a g2-epsps & gat dual transgenic glyphosate-resistant soybean line on the soil microbial community under field conditions affected by glyphosate application |
topic | Regular Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734404/ https://www.ncbi.nlm.nih.gov/pubmed/33162465 http://dx.doi.org/10.1264/jsme2.ME20056 |
work_keys_str_mv | AT yangminkai impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT wenzhongling impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT fazalaliya impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT huaxiaomei impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT xuxinhong impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT yintongming impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT qijinliang impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT yangrongwu impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT luguihua impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT hongzhi impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication AT yangyonghua impactofag2epspsgatdualtransgenicglyphosateresistantsoybeanlineonthesoilmicrobialcommunityunderfieldconditionsaffectedbyglyphosateapplication |