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Airway regulatory T cells are decreased in COPD with a rapid decline in lung function

BACKGROUND: Differences in the expression of regulatory T cells (Tregs) have been suggested to explain why some smokers develop COPD and some do not. Upregulation of Tregs in response to smoking would restrain airway inflammation and thus the development of COPD; while the absense of such upregulati...

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Autores principales: Eriksson Ström, Jonas, Pourazar, Jamshid, Linder, Robert, Blomberg, Anders, Lindberg, Anne, Bucht, Anders, Behndig, Annelie F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734742/
https://www.ncbi.nlm.nih.gov/pubmed/33317530
http://dx.doi.org/10.1186/s12931-020-01593-9
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author Eriksson Ström, Jonas
Pourazar, Jamshid
Linder, Robert
Blomberg, Anders
Lindberg, Anne
Bucht, Anders
Behndig, Annelie F.
author_facet Eriksson Ström, Jonas
Pourazar, Jamshid
Linder, Robert
Blomberg, Anders
Lindberg, Anne
Bucht, Anders
Behndig, Annelie F.
author_sort Eriksson Ström, Jonas
collection PubMed
description BACKGROUND: Differences in the expression of regulatory T cells (Tregs) have been suggested to explain why some smokers develop COPD and some do not. Upregulation of Tregs in response to smoking would restrain airway inflammation and thus the development of COPD; while the absense of such upregulation would over time lead to chronic inflammation and COPD. We hypothesized that—among COPD patients—the same mechanism would affect rate of decline in lung function; specifically, that a decreased expression of Tregs would be associated with a more rapid decline in FEV(1). METHODS: Bronchoscopy with BAL was performed in 52 subjects recruited from the longitudinal OLIN COPD study; 12 with COPD and a rapid decline in lung function (loss of FEV(1) ≥ 60 ml/year), 10 with COPD and a non-rapid decline in lung function (loss of FEV(1) ≤ 30 ml/year), 15 current and ex-smokers and 15 non-smokers with normal lung function. BAL lymphocyte subsets were determined using flow cytometry. RESULTS: The proportions of Tregs with regulatory function (FoxP3(+)/CD4(+)CD25(bright)) were significantly lower in COPD subjects with a rapid decline in lung function compared to those with a non-rapid decline (p = 0.019). This result was confirmed in a mixed model regression analysis in which adjustments for inhaled corticosteroid usage, smoking, sex and age were evaluated. No significant difference was found between COPD subjects and smokers or non-smokers with normal lung function. CONCLUSIONS: COPD subjects with a rapid decline in lung function had lower proportions of T cells with regulatory function in BAL fluid, suggesting that an inability to suppress the inflammatory response following smoking might lead to a more rapid decline in FEV(1). Trial registration Clinicaltrials.gov identifier NCT02729220
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spelling pubmed-77347422020-12-15 Airway regulatory T cells are decreased in COPD with a rapid decline in lung function Eriksson Ström, Jonas Pourazar, Jamshid Linder, Robert Blomberg, Anders Lindberg, Anne Bucht, Anders Behndig, Annelie F. Respir Res Research BACKGROUND: Differences in the expression of regulatory T cells (Tregs) have been suggested to explain why some smokers develop COPD and some do not. Upregulation of Tregs in response to smoking would restrain airway inflammation and thus the development of COPD; while the absense of such upregulation would over time lead to chronic inflammation and COPD. We hypothesized that—among COPD patients—the same mechanism would affect rate of decline in lung function; specifically, that a decreased expression of Tregs would be associated with a more rapid decline in FEV(1). METHODS: Bronchoscopy with BAL was performed in 52 subjects recruited from the longitudinal OLIN COPD study; 12 with COPD and a rapid decline in lung function (loss of FEV(1) ≥ 60 ml/year), 10 with COPD and a non-rapid decline in lung function (loss of FEV(1) ≤ 30 ml/year), 15 current and ex-smokers and 15 non-smokers with normal lung function. BAL lymphocyte subsets were determined using flow cytometry. RESULTS: The proportions of Tregs with regulatory function (FoxP3(+)/CD4(+)CD25(bright)) were significantly lower in COPD subjects with a rapid decline in lung function compared to those with a non-rapid decline (p = 0.019). This result was confirmed in a mixed model regression analysis in which adjustments for inhaled corticosteroid usage, smoking, sex and age were evaluated. No significant difference was found between COPD subjects and smokers or non-smokers with normal lung function. CONCLUSIONS: COPD subjects with a rapid decline in lung function had lower proportions of T cells with regulatory function in BAL fluid, suggesting that an inability to suppress the inflammatory response following smoking might lead to a more rapid decline in FEV(1). Trial registration Clinicaltrials.gov identifier NCT02729220 BioMed Central 2020-12-14 2020 /pmc/articles/PMC7734742/ /pubmed/33317530 http://dx.doi.org/10.1186/s12931-020-01593-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Eriksson Ström, Jonas
Pourazar, Jamshid
Linder, Robert
Blomberg, Anders
Lindberg, Anne
Bucht, Anders
Behndig, Annelie F.
Airway regulatory T cells are decreased in COPD with a rapid decline in lung function
title Airway regulatory T cells are decreased in COPD with a rapid decline in lung function
title_full Airway regulatory T cells are decreased in COPD with a rapid decline in lung function
title_fullStr Airway regulatory T cells are decreased in COPD with a rapid decline in lung function
title_full_unstemmed Airway regulatory T cells are decreased in COPD with a rapid decline in lung function
title_short Airway regulatory T cells are decreased in COPD with a rapid decline in lung function
title_sort airway regulatory t cells are decreased in copd with a rapid decline in lung function
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734742/
https://www.ncbi.nlm.nih.gov/pubmed/33317530
http://dx.doi.org/10.1186/s12931-020-01593-9
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