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Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation
[Image: see text] The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735732/ https://www.ncbi.nlm.nih.gov/pubmed/33236630 http://dx.doi.org/10.1021/acs.jafc.9b07251 |
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author | Osorio-Macías, Daniel E. Song, Dongsup Thuvander, Johan Ferrer-Gallego, Raúl Choi, Jaeyeong Peñarrieta, J. Mauricio Nilsson, Lars Lee, Seungho Bergenståhl, Björn |
author_facet | Osorio-Macías, Daniel E. Song, Dongsup Thuvander, Johan Ferrer-Gallego, Raúl Choi, Jaeyeong Peñarrieta, J. Mauricio Nilsson, Lars Lee, Seungho Bergenståhl, Björn |
author_sort | Osorio-Macías, Daniel E. |
collection | PubMed |
description | [Image: see text] The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (AsFlFFF–MALS–dRI–UV) for the fractionation and determination of the molar mass, the hydrodynamic radius, and the apparent densities of the aggregates and macromolecules present in wine samples. The results from a set of six Argentinian high-altitude wines showed two main populations: the first population composed of wine colloids with higher UV-specific absorptivity and the second population composed of polysaccharides, such as arabinogalactans. The conformation results showed that population 1 consists of small and dense particles, while population 2 showed high molar masses and lower densities. The results demonstrated the use of AsFlFFF as a new, effective method for the fractionation and characterization of wine colloids and wine macromolecules in red wines with further potential applications. |
format | Online Article Text |
id | pubmed-7735732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-77357322020-12-15 Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation Osorio-Macías, Daniel E. Song, Dongsup Thuvander, Johan Ferrer-Gallego, Raúl Choi, Jaeyeong Peñarrieta, J. Mauricio Nilsson, Lars Lee, Seungho Bergenståhl, Björn J Agric Food Chem [Image: see text] The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (AsFlFFF–MALS–dRI–UV) for the fractionation and determination of the molar mass, the hydrodynamic radius, and the apparent densities of the aggregates and macromolecules present in wine samples. The results from a set of six Argentinian high-altitude wines showed two main populations: the first population composed of wine colloids with higher UV-specific absorptivity and the second population composed of polysaccharides, such as arabinogalactans. The conformation results showed that population 1 consists of small and dense particles, while population 2 showed high molar masses and lower densities. The results demonstrated the use of AsFlFFF as a new, effective method for the fractionation and characterization of wine colloids and wine macromolecules in red wines with further potential applications. American Chemical Society 2020-11-25 2020-12-09 /pmc/articles/PMC7735732/ /pubmed/33236630 http://dx.doi.org/10.1021/acs.jafc.9b07251 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Osorio-Macías, Daniel E. Song, Dongsup Thuvander, Johan Ferrer-Gallego, Raúl Choi, Jaeyeong Peñarrieta, J. Mauricio Nilsson, Lars Lee, Seungho Bergenståhl, Björn Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation |
title | Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation |
title_full | Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation |
title_fullStr | Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation |
title_full_unstemmed | Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation |
title_short | Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation |
title_sort | fractionation of nanoparticle matter in red wines using asymmetrical flow field-flow fractionation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735732/ https://www.ncbi.nlm.nih.gov/pubmed/33236630 http://dx.doi.org/10.1021/acs.jafc.9b07251 |
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