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Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins
In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing. Additionally, no study has reported the possibili...
Autores principales: | Tanida, Isei, Furuta, Yoko, Yamaguchi, Junji, Kakuta, Soichiro, Oliva Trejo, Juan Alejandro, Uchiyama, Yasuo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736269/ https://www.ncbi.nlm.nih.gov/pubmed/33318540 http://dx.doi.org/10.1038/s41598-020-78879-x |
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