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The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia
Using genome-wide transcriptome analysis by RNA sequencing of first trimester plasma RNA, we tested whether the identification of pregnancies at risk of developing pre-eclampsia with or without preterm birth or growth restriction is possible between weeks 9–14, prior to the appearance of clinical sy...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736279/ https://www.ncbi.nlm.nih.gov/pubmed/33318568 http://dx.doi.org/10.1038/s41598-020-79008-4 |
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author | Manders, Vera Visser, Allerdien Keijser, Remco Min, Naomi Poutsma, Ankie Mulders, Joyce van den Berkmortel, Tarah Hortensius, Marjolein Jongejan, Aldo Pajkrt, Eva Sistermans, Erik A. Sie, Daoud Best, Myron G. Würdinger, Tom de Boer, Marjon Afink, Gijs Oudejans, Cees |
author_facet | Manders, Vera Visser, Allerdien Keijser, Remco Min, Naomi Poutsma, Ankie Mulders, Joyce van den Berkmortel, Tarah Hortensius, Marjolein Jongejan, Aldo Pajkrt, Eva Sistermans, Erik A. Sie, Daoud Best, Myron G. Würdinger, Tom de Boer, Marjon Afink, Gijs Oudejans, Cees |
author_sort | Manders, Vera |
collection | PubMed |
description | Using genome-wide transcriptome analysis by RNA sequencing of first trimester plasma RNA, we tested whether the identification of pregnancies at risk of developing pre-eclampsia with or without preterm birth or growth restriction is possible between weeks 9–14, prior to the appearance of clinical symptoms. We implemented a metaheuristic approach in the self-learning SVM algorithm for differential gene expression analysis of normal pregnancies (n = 108), affected pregnancies (n = 34) and non-pregnant controls (n = 19). Presymptomatic candidate markers for affected pregnancies were validated by RT-qPCR in first trimester samples (n = 34) from an independent cohort. PRKG1 was significantly downregulated in a subset of pregnancies with birth weights below the 10thpercentile as shared symptom. The NRIP1/ZEB2 ratio was found to be upregulated in pregnancies with pre-eclampsia or trisomy 21. Complementary quantitative analysis of both the linear and circular forms of NRIP1 permitted discrimination between pre-eclampsia and trisomy 21. Pre-eclamptic pregnancies showed an increase in linear NRIP1 compared to circular NRIP1, while trisomy 21 pregnancies did not. |
format | Online Article Text |
id | pubmed-7736279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77362792020-12-15 The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia Manders, Vera Visser, Allerdien Keijser, Remco Min, Naomi Poutsma, Ankie Mulders, Joyce van den Berkmortel, Tarah Hortensius, Marjolein Jongejan, Aldo Pajkrt, Eva Sistermans, Erik A. Sie, Daoud Best, Myron G. Würdinger, Tom de Boer, Marjon Afink, Gijs Oudejans, Cees Sci Rep Article Using genome-wide transcriptome analysis by RNA sequencing of first trimester plasma RNA, we tested whether the identification of pregnancies at risk of developing pre-eclampsia with or without preterm birth or growth restriction is possible between weeks 9–14, prior to the appearance of clinical symptoms. We implemented a metaheuristic approach in the self-learning SVM algorithm for differential gene expression analysis of normal pregnancies (n = 108), affected pregnancies (n = 34) and non-pregnant controls (n = 19). Presymptomatic candidate markers for affected pregnancies were validated by RT-qPCR in first trimester samples (n = 34) from an independent cohort. PRKG1 was significantly downregulated in a subset of pregnancies with birth weights below the 10thpercentile as shared symptom. The NRIP1/ZEB2 ratio was found to be upregulated in pregnancies with pre-eclampsia or trisomy 21. Complementary quantitative analysis of both the linear and circular forms of NRIP1 permitted discrimination between pre-eclampsia and trisomy 21. Pre-eclamptic pregnancies showed an increase in linear NRIP1 compared to circular NRIP1, while trisomy 21 pregnancies did not. Nature Publishing Group UK 2020-12-14 /pmc/articles/PMC7736279/ /pubmed/33318568 http://dx.doi.org/10.1038/s41598-020-79008-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Manders, Vera Visser, Allerdien Keijser, Remco Min, Naomi Poutsma, Ankie Mulders, Joyce van den Berkmortel, Tarah Hortensius, Marjolein Jongejan, Aldo Pajkrt, Eva Sistermans, Erik A. Sie, Daoud Best, Myron G. Würdinger, Tom de Boer, Marjon Afink, Gijs Oudejans, Cees The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia |
title | The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia |
title_full | The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia |
title_fullStr | The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia |
title_full_unstemmed | The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia |
title_short | The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia |
title_sort | bivariate nrip1/zeb2 rna marker permits non-invasive presymptomatic screening of pre-eclampsia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736279/ https://www.ncbi.nlm.nih.gov/pubmed/33318568 http://dx.doi.org/10.1038/s41598-020-79008-4 |
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