Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

In the yeast Saccharomyces cerevisiae, terminator sequences not only terminate transcription but also affect expression levels of the protein-encoded upstream of the terminator. The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has frequently been used as a platform for metaboli...

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Detalles Bibliográficos
Autores principales: Ito, Yoichiro, Terai, Goro, Ishigami, Misa, Hashiba, Noriko, Nakamura, Yasuyuki, Bamba, Takahiro, Kumokita, Ryota, Hasunuma, Tomohisa, Asai, Kiyoshi, Ishii, Jun, Kondo, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736810/
https://www.ncbi.nlm.nih.gov/pubmed/33257988
http://dx.doi.org/10.1093/nar/gkaa1066

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